Abstract in English:
Brucella melitensis and Brucella abortus are known to cause brucellosis in camels. It is especially seen in camels raised together with ruminants in regions where brucellosis is endemic. However, since this disease causes only a few clinical signs, unlike cattle, it is challenging to diagnose camel brucellosis. While camels bred in Asia and Africa are used for milk, meat, and transportation purposes, camel breeding in Turkey consists of small male camel populations raised under special conditions for cultural purposes. The aim of this study was to investigate the seroprevalence of brucellosis in camels in the Aegean region of Turkey using three serological tests – Rose Bengal plate test (RBPT), serum agglutination test (SAT), and complement fixation test (CFT) – and to compare their diagnostic performance. A total of 76 camel sera (72 Camelus dromedarius and four Camelus bactrianus) were collected from three cities (İzmir, Aydın, and Denizli) via random sampling. All sera were tested simultaneously using RBPT, SAT, and CFT. A positive control serum from a camel vaccinated with conjunctival B. abortus S19 and a negative control serum from a brucellosis-free newborn camel calf. It was determined that all 76 camel sera screened, except the positive control serum, gave negative results in RBP, SA, and CF tests. This comprehensive serological survey represents the first documented evidence of a brucellosis-free status in the camel population in Turkey. The findings suggest that the isolated and special conditions in which the camels are raised, the lack of contact with ruminants, and the predominantly male camel population contribute to the low risk of brucellosis in this region. These results highlight the importance of region-specific management practices in controlling camel brucellosis.
Abstract in Portuguese:
Brucella melitensis e Brucella abortus são conhecidas por causar brucelose em camelos. É especialmente observada em camelos criados em conjunto com ruminantes em regiões onde a brucelose é endêmica. No entanto, como essa doença causa apenas alguns sinais clínicos, ao contrário do que acontece com o gado bovino, o diagnóstico da brucelose em camelos é desafiador. Enquanto a criação de camelos na Ásia e na África é principalmente para produção de leite, carne e transporte, a criação de camelos na Turquia consiste em pequenas populações de camelos machos criados em condições especiais para fins culturais. O objetivo deste estudo foi investigar a soroprevalência da brucelose em camelos na região do Mar Egeu, na Turquia, utilizando três testes sorológicos – teste de placa rosa bengala (TPRB), teste de aglutinação sorológica (TAS) e teste de fixação de complemento (TFC) – e comparar seus desempenhos diagnósticos. Soros de 76 camelos (72 Camelus dromedarius e quatro Camelus bactrianus) foram coletados em três cidades (Izmir, Aydın e Denizli) por meio de amostragem aleatória. Todos os soros foram testados simultaneamente com RBPT, SAT e CFT. Foram incluídos um soro de controle positivo de um camelo vacinado com B. abortus S19 conjuntival e um soro de controle negativo de um camelo recém-nascido livre de brucelose. Foi determinado que todos os 76 soros de camelo examinados, exceto o soro de controle positivo, apresentaram resultados negativos nos testes PRB, AS e FC. Este abrangente levantamento sorológico representa a primeira evidência documentada de um status livre de brucelose na população de camelos na Turquia. Os resultados sugerem que as condições isoladas e especiais em que os camelos são criados, a falta de contato com ruminantes e a população predominantemente masculina de camelos contribuem para o baixo risco de brucelose nesta região. Esses resultados destacam a importância de práticas de manejo específicas para cada região no controle da brucelose em camelos.
Abstract in English:
The study aimed to investigate the distribution and expression of nesfatin-1 in Bactrian camels and its impact on adipocyte glucose metabolism. Polyclonal antibodies against a single antigenic epitope of NUCB2/nesfatin-1 protein were prepared. Nesfatin-1 expression was detected in various adipose tissues, including the hypothalamus, humps, stomach, duodenum, jejunum, ileum, cecum, colon, rectum, pancreas, liver, and abdomen, using Western blot and qRT-PCR. Glucose uptake levels, pyruvic acid content, and hexokinase and phosphofructokinase activities were measured in cultured 3T3-L1 preadipocytes treated with nesfatin-1 in a high glucose state using a non-radiofluorimetric assay. The results showed that nesfatin-1 was found in all Bactrian camel tissues, with higher adipose tissue and pancreas expression. However, qRT-PCR analysis revealed higher expression of nesfatin-1 mRNA in the abdominal fat and liver. After nesfatin-1 treatment, a significant decrease (p<0.05) was observed in glucose uptake levels, hexokinase, and phosphofructokinase activities in 3T3-L1 cells, with a significant increase (p<0.05) in their pyruvic acid content. These findings suggest that nesfatin-1 is expressed at high levels in the abdominal fat of Bactrian camels and significantly affects adipocyte glucose metabolism by regulating blood glucose levels, inhibiting adipocyte differentiation, and promoting lipid droplet hydrolysis to provide energy for the organism, which may relate to the Bactrian camel’s ability to adapt to harsh environments.
Abstract in Portuguese:
The study aimed to investigate the distribution and expression of nesfatin-1 in Bactrian camels and its impact on adipocyte glucose metabolism. Polyclonal antibodies against a single antigenic epitope of NUCB2/nesfatin-1 protein were prepared. Nesfatin-1 expression was detected in various adipose tissues, including the hypothalamus, humps, stomach, duodenum, jejunum, ileum, cecum, colon, rectum, pancreas, liver, and abdomen, using Western blot and qRT-PCR. Glucose uptake levels, pyruvic acid content, and hexokinase and phosphofructokinase activities were measured in cultured 3T3-L1 preadipocytes treated with nesfatin-1 in a high glucose state using a non-radiofluorimetric assay. The results showed that nesfatin-1 was found in all Bactrian camel tissues, with higher adipose tissue and pancreas expression. However, qRT-PCR analysis revealed higher expression of nesfatin-1 mRNA in the abdominal fat and liver. After nesfatin-1 treatment, a significant decrease (p<0.05) was observed in glucose uptake levels, hexokinase, and phosphofructokinase activities in 3T3-L1 cells, with a significant increase (p<0.05) in their pyruvic acid content. These findings suggest that nesfatin-1 is expressed at high levels in the abdominal fat of Bactrian camels and significantly affects adipocyte glucose metabolism by regulating blood glucose levels, inhibiting adipocyte differentiation, and promoting lipid droplet hydrolysis to provide energy for the organism, which may relate to the Bactrian camel’s ability to adapt to harsh environments.
Abstract in English:
This study discusses the epidemiological, clinical, and pathological characteristics of two outbreaks of ocular infection in ostriches (Struthio camelus) caused by the trematode Philophthalmus sp. The outbreaks occurred in the states of Ceará and Rio Grande do Norte in Northeast Brazil. Ostrich farming was categorized as semi-intensive farming with free access to the weir and river. The clinical symptoms for infection included nictitating membrane inflammation, epiphora (tearing), eyelid edema, ocular congestion, eyeball destruction, emaciation, and death, and the infection period ranged from 8 to 16 months. Macroscopically, thickening of the eyelids and conjunctiva were observed, with orange parasites infestation. Histologically, the lesions were characterized as lymphoplasmacytic and heterophilic, multifocal to coalescing, moderate, chronic conjunctivitis associated with hyperplasia and ectasia of the Harderian gland, and numerous parasites. The parasites were oval and flattened with an oral suction cup, acetabulum, and thick cuticle containing spicules. The diagnosis of philophthalmiasis was based on epidemiological, clinical, and pathological findings associated with the morphological characteristics of the parasites present in the conjunctival sacs which were similar to the trematode Philophthalmus sp. Philophthalmiasis can occur in ostriches raised on urban and rural properties in Northeastern Brazil, probably associated with the contact of birds with contaminated water, and is characterized by severe conjunctivitis that can result in the loss of the eyeball and death of the animals.
Abstract in Portuguese:
Descrevem-se as características epidemiológicas, clínicas e patológicas de dois surtos de infecção ocular pelo trematódeo Philophthalmus sp. em avestruzes ocorridos nos estados do Ceará e Rio Grande do Norte no Nordeste do Brasil. As propriedades tinham em comum, a criação semi-intensiva e livre acesso a açude e rio. Os sinais clínicos incluíam protrusão da membrana nictante, lacrimejamento, edema palpebral, congestão ocular, destruição do globo ocular, emagrecimento e morte. A evolução clínica variou de oito a 16 meses. Macroscopicamente, observou-se espessamento das pálpebras e conjuntivas associado a numerosas estruturas parasitárias alaranjadas. No segundo surto, as lesões eram mais graves e consistiam em perda do globo ocular direito e anexos. Histologicamente, as lesões caracterizavam-se por conjuntivite linfoplasmocítica e heterofílica, multifocal a coalescente, moderada, crônica associada a hiperplasia e ectasia da glândula de Harderian e numerosas estruturas parasitárias. Os parasitas eram ovalados, achatados com ventosa oral e acetábulo e cutícula espessa contendo espículas. O diagnóstico de filoftalmíase foi realizado com base nos achados epidemiológicos, clínicos e patológicos associadas as características morfológicas dos parasitas presentes nos sacos conjuntivais compatíveis com o trematódeo Philophthalmus sp. A filoftalmíase pode ocorrer em avestruzes criados em propriedades urbanas e rurais no Nordeste do Brasil, provavelmente associado ao contato das aves com água contaminada e caracteriza-se por quadros de conjuntivite grave que pode resultar com a perda do globo ocular e morte dos animais.
Abstract in English:
ABSTRACT.- Seixas J.N., Orlando D.R., Wouters F., Wouters A.T.B., Varaschin M.S. & Raymundo D.L. 2017. [Pathological aspects of diminazene aceturate toxicosis in american camelids.] Aspectos patológicos da intoxicação por aceturato de diminazeno em camelídeos sul-americanos. Pesquisa Veterinária Brasileira 37(12):1509-1513. Setor de Patologia Veterinária, Universidade Federal de Lavras, Av. Doutor Sylvio Menicucci 1001, Cx. Postal 3037, Kennedy, Lavras, MG 37200-000, Brasil. E-mail: josiseixas@dsa.ufla.br
Diminazene aceturate is a synthetic chemotherapeutic drug commonly used in veterinary medicine for the treatment of diseases caused by hematozoan parasites. However, side effects as severe neurological disorders and death can occur. The raising of american camelids is a recent activity in Brazil, requiring knowledge about diseases that affect these species, in order to avoid misguided conducts. In a herd of ten camelids (six llamas and four alpacas) six showed clinical signs and five died; only a llama with mild signs recovered. The clinical signs included apathy, difficulty to stand up, staggering gait, weakness, down head and drooping the head laterally, dyspnea and drooling of saliva, observed from 18 hours after use of the drug. At necropsy and histopathological examination was found bilateral and symmetrical hemorrhagic encephalopathy, more severe in brainstem and thalamus. This paper describes the main lesions observed in an outbreak of diminazene aceturate poisoning in alpacas (Lama pacos) and llamas (Lama glama) and alert breeders and veterinarians about the risk of poisoning by this drug in american camelids.
Abstract in Portuguese:
RESUMO.- Seixas J.N., Orlando D.R., Wouters F., Wouters A.T.B., Varaschin M.S. & Raymundo D.L. 2017. [Pathological aspects of diminazene aceturate toxicosis in american camelids.] Aspectos patológicos da intoxicação por aceturato de diminazeno em camelídeos sul-americanos. Pesquisa Veterinária Brasileira 37(12):1509-1513. Setor de Patologia Veterinária, Universidade Federal de Lavras, Av. Doutor Sylvio Menicucci 1001, Cx. Postal 3037, Kennedy, Lavras, MG 37200-000, Brasil. E-mail: josiseixas@dsa.ufla.br
Aceturato de diminazeno é um fármaco quimioterápico sintético comumente usado na medicina veterinária para o tratamento de doenças causadas por parasitos hematozoários. Entretanto, seu uso pode levar a efeitos colaterais, como alterações neurológicas graves e morte. A criação de camelídeos é uma atividade recente no Brasil, fazendo-se necessário conhecer mais sobre as doenças que acometem essas espécies. De dez camelídeos (seis lhamas e quatro alpacas) da propriedade, seis tiveram sinais clínicos e, destes, apenas uma lhama com manifestações leves recuperou-se. Os sinais clínicos incluíam apatia, andar cambaleante, fraqueza, sialorreia, cabeça baixa e pendida lateralmente, dificuldade em levantar e dispneia, observados a partir de 18 horas após o uso do medicamento. À necropsia e ao exame histopatológico foram observadas alterações de encefalopatia hemorrágica bilateral e simétrica, mais graves em tronco encefálico e tálamo. Este trabalho descreve as principais lesões observadas em um surto de intoxicação por diminazeno em alpacas (Lama pacos) e lhamas (Lama glama) e alerta criadores e veterinários sobre o risco de intoxicação por aceturato de diminazeno em camelídeos sul-
americanos.
Abstract in English:
Tuberculosis is a debilitating infecto-contagious disease, caused by an acid-fast bacillus (AFB) that belong to different species of the Mycobacterium tuberculosis complex (MTC). Mycobacteriosis are important diseases in veterinary medicine because of their zoonotic potential and worldwide distribution, affecting all classes of vertebrates. In wild animals the mycobacteriosis have been reported mainly as a problem in captivity. There are also reports in free-ranging wildlife, endangering and hampering tuberculosis erradication programs in animal production. The diagnosis of the disease in wildlife is usually postmortem, because the tuberculin test is not standardized for wildlife species, and also it is not reliable for screening. The postmortem diagnosis is based on macroscopic findings, microscopic detection of AFB at Ziehl-Neelsen staining (ZN), and mainly isolation and identification of the agent. However, only gross and microscopic exams do not allow to distinguish the species of Mycobacterium involved. The immunostaining with polyclonal anti-Mycobacterium tuberculosis confirms tuberculosis infection, but is not specific; there may be marking of other mycobacteria. The aim of this study was to describe the histologic findings, of ZN staining and immunohistochemistry technique (IHC) of 13 cases of wildlife herbivores diagnosed with tuberculosis in the Setor de Patologia Veterinária of the Universidade Federal do Rio Grande do Sul (SPV-UFRGS) during the 2003- 2015 period. Formalin fixed paraffin embedded tissues were recut, stained with hematoxylin and eosin and ZN, and samples were submitted to the IHC (anti-M. tuberculosis marking, streptavidin-biotin peroxidase method). All animals included were adults living in captivity and belonged to the following species: llama (5/13), sambar deer (4/13), camel (1/13), red deer (1/13), Brazilian tapir (1/13) and Nilgai antelope (1/13). The IHC revealed immunostaining of accentuated (4/13), moderate (4/13) and discrete (4/13) intensity, except in a case with insufficient material. The histological features, findings in ZN staining and IHC in the wild herbivores with tuberculosis lesions allowed the diagnosis of infection with Mycobacterium sp., turning into fast and efficient methods of diagnosis, which can help to prevent the spread of this disease in animals from the same herd.
Abstract in Portuguese:
A tuberculose é uma enfermidade infectocontagiosa, debilitante, causada por bacilos álcool-ácido resistentes (BAAR), pertencentes ao complexo Mycobacterium tuberculosis (CMT). As micobacterioses têm importância em Medicina Veterinária devido ao seu potencial zoonótico e sua distribuição mundial, afetando todas as classes de vertebrados. Em animais selvagens as micobacterioses têm sido um problema relatado principalmente em cativeiro. Contudo, há relatos de sua ocorrência também em animais de vida livre, colocando em risco e dificultando os programas de erradicação da tuberculose em animais de produção. O diagnóstico nas espécies selvagens em geral é post mortem, uma vez que o teste de tuberculina não está padronizado para essas espécies, assim como não é confiável para triagem. São consideradas para o diagnóstico lesões de necropsia, observação microscópica de BAAR na coloração de Ziehl-Neelsen (ZN) e, principalmente, isolamento e identificação do agente. No entanto, apenas os achados morfológicos macro e microscópicos não permitem distinguir a espécie de Mycobacterium envolvida. A técnica de imuno-histoquímica (IHQ) com anticorpo policlonal anti-M. tuberculosis confirma a infecção pelo CMT, mas não é específica, pois pode ocorrer marcação de outras micobactérias. As características histológicas, os achados na coloração de ZN e na IHQ de 13 casos de herbívoros selvagens diagnosticados com tuberculose no Setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul (SPV-UFRGS) no período de 2003 a 2015 são descritos. A partir das amostras em blocos de parafina foram confeccionadas novas lâminas histológicas, coradas com hematoxilina e eosina (HE) e ZN. Cortes foram submetidos à técnica de IHQ para detecção do CMT. Todos os animais eram adultos, provenientes de cativeiro e incluíram lhama (5/13), cervo sambar (4/13), camelo (1/13), cervo vermelho (1/13), anta brasileira (1/13) e antílope Nilgai (1/13). Na IHQ observou-se imunomarcação acentuada (4/13), moderada (4/13) ou discreta (4/13), exceto em um caso, em que não havia quantidade suficiente de material. As características histológicas, bem como os achados na coloração de ZN e na técnica de IHQ confirmaram o diagnóstico de infecção por Mycobacterium sp. e foram considerados métodos rápidos e eficientes, de forma que podem ajudar na prevenção da disseminação da doença em animais.
Abstract in English:
ABSTRACT.- Neira R.H., Estruc T.M., Nascimento R.M., Santos-Sousa C.A., Souza-Junior P. & Abidu-Figueiredo M. 2016. [Origin and main ramifications of the cranial and caudal mesenteric arteries in ostrich (Struthio camelus Linnaeus, 1758).] Origem e principais ramificações das artérias mesentéricas cranial e caudal em avestruz (Struthio camelus Linnaeus, 1758). Pesquisa Veterinária Brasileira 36(9):912-918. Departamento de Anatomia, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Rodovia BR-465 Km7, Seropédica, RJ 23890-000, Brazil. E-mail: marceloabidu@gmail.com
The mesenteric arteries of birds are important for the irrigation of the digestive tract and are associated with weight gain and food conversion. This study aimed to describe the origins, skeletopy, measures and main branches of cranial and caudal mesenteric arteries in ostriches. Forty-one cadavers of ostrich chicks, 23 males and 18 females, obtained from a farmer after natural death. The cadavers were fixed with 10% formaldehyde solution and their vascular system was filled with colored Petrolatex® S-65. The cranial and caudal mesenteric arteries and its proximal branches were dissected in situ and measured with a digital caliper. The mesenteric artery had an average length of 3.68cm±1.04 and emerged from the descending aorta at the level of the eighth thoracic vertebra in most cases; it branched into jejunal and ileocecal arteries. The jejunal artery sent a mean of 14 (14.04±2.08) branches to the jejunum. The ileocecal artery sent a rectal branch and another branch that irrigated ileum, cecum and rectum. In a male specimen the ileocecal artery was originated from the celiac artery. The caudal mesenteric artery emerged in the terminal portion of the descending aorta predominantly at the level of the 4th and 6th sacrocaudal vertebrae. Near the caudal end of the kidney it issued the cranial and caudal branches. The first irrigated the rectum and anastomosed with the rectal branch of the cranial mesenteric artery; the second irrigated the final part of the rectum, cloaca and cloacal bursa. There was no significant difference (p<0.05) between measurements, skeletopy and number of branches of the arteries between genders.
Abstract in Portuguese:
RESUMO.- Neira R.H., Estruc T.M., Nascimento R.M., Santos-Sousa C.A., Souza-Junior P. & Abidu-Figueiredo M. 2016. [Origin and main ramifications of the cranial and caudal mesenteric arteries in ostrich (Struthio camelus Linnaeus, 1758).] Origem e principais ramificações das artérias mesentéricas cranial e caudal em avestruz (Struthio camelus Linnaeus, 1758). Pesquisa Veterinária Brasileira 36(9):912-918. Departamento de Anatomia, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Rodovia BR-465 Km7, Seropédica, RJ 23890-000, Brazil. E-mail: marceloabidu@gmail.com
As artérias mesentéricas das aves são importantes para a irrigação do aparelho digestório e encontram-se associadas ao ganho de peso e conversão alimentar. Objetivou-se descrever as origens, esqueletopias, medidas e principais ramificações das artérias mesentéricas cranial e caudal em avestruzes. Foram utilizados 41 cadáveres de filhotes de avestruzes, 23 machos e 18 fêmeas, obtidos de um criadouro após morte natural. Os cadáveres foram fixados com formaldeído a 10% e tiveram o sistema vascular preenchido com Petrolatex® S-65 colorido. As artérias mesentéricas, cranial e caudal e seus ramos proximais foram dissecados “in situ” e medidas com paquímetro digital. A artéria mesentérica cranial teve comprimento médio de 3,68 ± 1,04 cm e surgiu da aorta descendente ao nível da oitava vértebra torácica na maioria dos casos. Ramificou-se em artérias jejunal e ileocecal. A artéria jejunal ofereceu média de 14,04 ±2,08 ramos ao jejuno e a artéria ileocecal originou um ramo retal e outro que se bifurcou para derivar ramos para íleo, ceco e reto. Em um espécime macho a artéria ileocecal foi ramo da artéria celíaca. A artéria mesentérica caudal originou-se na porção terminal da aorta descendente predominantemente ao nível das 4ª e 6ª vértebras sacro-caudais. Perto da extremidade caudal do rim emitiu os ramos cranial e caudal. O primeiro irrigou o reto e anastomosou-se com ramo retal da artéria mesentérica cranial; o segundo irrigou a porção final do reto, cloaca e bolsa cloacal. Não houve diferença significativa (p<0,05) entre as medidas, esqueletopia e número de ramificações das artérias entre os sexos.
Abstract in English:
ABSTRACT.- Tivane C., Rodrigues M.N., Soley J.T. & Groenwald H.B. 2011. Gross anatomical features of the oropharyngeal cavity of the ostrich (Struthio camelus). Pesquisa Veterinária Brasileira 31(6):543-550. Departamento Pré-Clinico, Faculdade de Veterinária, Universidade Eduardo Mondlane, Avenida de Moçambique, Km 1.5, Maputo, Mozambique. E-mail: catarina.tivane@uem.mz
Most descriptions of the ostrich oropharynx and oesophagus are superficial and supply little meaningful morphological data. The aim of this investigation is describe the ostrich oropharingeal cavity, in order to supply the deficiency of macroscopic data about this important animal. Five heads of 12 to 14-month-old ostriches of either sex were anatomically dissected to expose the oropharynx. The ostrich oropharynx was “bell-shaped” composed by the maxillary and mandibular ramphoteca. The roof and floor presented two distinct regions different in colour of the mucosa. The rostral region was pale pink contrasting to creamy-pink coloured caudal region. The median longitudinal ridge extended rostrally from the apex of the choana to the tip of the beak in the roof and it is clearly more prominent and rigid than the homolog in the floor that appeared thin and stretched rostrally, continuing caudally surrounding the tongue and the laryngeal mound eventually merging with the oesophageal mucosa. The floor was formed by the interramal region, tongue and laryngeal mound containing shield-shaped glottis. It can be concluded that the present study, in addition to confirming the basic features of the oropharynx previously described for the ostrich, clarified the contradictory information presented in the literature and also provided new, unreported morphological data, some of which may be important when studying nutrition and health in these birds.
Abstract in Portuguese:
RESUMO.- Tivane C., Rodrigues M.N., Soley J.T. & Groenwald H.B. 2011. Gross anatomical features of the oropharyngeal cavity of the ostrich (Struthio camelus). [Características macroscópicas da cavidade orofaríngea de avestruz (Struthio camelus).] Pesquisa Veterinária Brasileira 31(6):543-550. Departamento Pré-Clinico, Faculdade de Veterinária, Universidade Eduardo Mondlane, Avenida de Moçambique, Km 1.5, Maputo, Mozambique. E-mail: catarina.tivane@uem.mz
Os estudos já realizados sobre a cavidade orofaríngea da avestruz são escassos e não elucidam completamente sua morfologia. O objetivo desse estudo foi descrever macroscopicamente a cavidade orofaríngea de avestruzes com o intuito de suprir a deficiência de informação nesta espécie. Foram utilizadas 5 cabeças de avestruzes com doze a quatorze meses de idade dissecadas seguindo padrões anatômicos de modo a expor a orofaringe. A orofaringe de avestruzes tem formato de sino e é composta pelas ranfotecas maxilar e mandibular. O teto e o assoalho apresentavam duas regiões distintas diferindo quanto à coloração da mucosa. A porção mais rostral era de coloração rosa pálida contrastando com a coloração mais fortemente rosada da porção mais caudal. A ruga palatina mediana estendia-se rostralmente do ápice da coana até a ponta do bico. No teto esta era mais proeminente e rígida que a do assoalho que se apresentava delgada e se estendia ao longo da porção rostral da região interramal, continuando caudalmente ao redor da coana e laringe e estendendo-se até o esôfago. O assoalho estava formado pela região interramal, língua e uma laringe com uma glote em forma de escudo. Pode-se concluir o presente estudo, além de confirmar as características básicas da orofaringe da avestruz previamente descritas, clarificam a informação contraditória presente na literatura e também novas informações morfológicas não previamente descritas são destacadas de forma a amparar outros estudos sobre a nutrição e saúde destas aves.
Abstract in English:
ABSTRACT.- Miranda R.L., Mundim A.V., Costa À.S., Guimarães R.V. & Silva F.O.C. 2008. Serum biochemistry of 4-day-old ostriches (Struthio camelus). Pesquisa Veterinária Brasileira 28(9):423-426. Laboratório Clínico Veterinário da Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Pará 1720, Bloco 2T, Campus Umuarama, Uberlândia, MG 38400-902, Brazil. E-mail: renatavetufu@yahoo.com.br
An analysis was made of 30 four-day-old ostriches to evaluate their protein, metabolite, mineral, and serum enzyme profiles, to correlate them with the birds’ sex. The values obtained were: Total proteins 3.59±0.72g/dL, albumin 1.04±0.14g/dL, globulins 2.51±0.56g/dL, A:G ratio 0.43± 0.07, total cholesterol 615.10±101.15mg/dL, high density lipoprotein cholesterol (HDL-C) 132.72±20.33mg/dL, low density lipoprotein cholesterol (LDL-C) 454.93±90.81mg/dL, very low density lipoprotein cholesterol (VLDL-C) 27.45±9.96mg/dL, triglycerides 137.23±49.78mg/dL, uric acid 6.24±2.15mg/dL, urea 18.27±12.33mg/dL, creatinine 0.30±0.04mg/dL, total calcium 9.38± 0.76mg/dL, ionized calcium 7.17±0.64mg/dL, phosphorus 6.96±0.91mg/dL, Ca:P ratio 1.37±0.21, iron 24.74±13.02µg/dL, sodium 142.03±6.17mEq/L, chlorides 109.59± 4.99mEq/L, aspartate aminotransferase (AST) 200.67±31.42 U/L, alanine aminotransferase (ALT) 3.90±1.92 U/L, g-glutamyltransferase (GGT) 1.18±0.73 U/L, alkaline phosphatase (ALP) 597.30± 231.36 U/L, and creatine kinase (CK) 2348.30±755.60 U/L. Males and females showed significant differences in total proteins, globulins, alkaline phosphatase, A:G ratio, and uric acid.
Abstract in Portuguese:
ABSTRACT.- Miranda R.L., Mundim A.V., Costa À.S., Guimarães R.V. & Silva F.O.C. 2008. Serum biochemistry of 4-day-old ostriches (Struthio camelus). Pesquisa Veterinária Brasileira 28(9):423-426. Laboratório Clínico Veterinário da Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Pará 1720, Bloco 2T, Campus Umuarama, Uberlândia, MG 38400-902, Brazil. E-mail: renatavetufu@yahoo.com.br
An analysis was made of 30 four-day-old ostriches to evaluate their protein, metabolite, mineral, and serum enzyme profiles, to correlate them with the birds’ sex. The values obtained were: Total proteins 3.59±0.72g/dL, albumin 1.04±0.14g/dL, globulins 2.51±0.56g/dL, A:G ratio 0.43± 0.07, total cholesterol 615.10±101.15mg/dL, high density lipoprotein cholesterol (HDL-C) 132.72±20.33mg/dL, low density lipoprotein cholesterol (LDL-C) 454.93±90.81mg/dL, very low density lipoprotein cholesterol (VLDL-C) 27.45±9.96mg/dL, triglycerides 137.23±49.78mg/dL, uric acid 6.24±2.15mg/dL, urea 18.27±12.33mg/dL, creatinine 0.30±0.04mg/dL, total calcium 9.38± 0.76mg/dL, ionized calcium 7.17±0.64mg/dL, phosphorus 6.96±0.91mg/dL, Ca:P ratio 1.37±0.21, iron 24.74±13.02µg/dL, sodium 142.03±6.17mEq/L, chlorides 109.59± 4.99mEq/L, aspartate aminotransferase (AST) 200.67±31.42 U/L, alanine aminotransferase (ALT) 3.90±1.92 U/L, g-glutamyltransferase (GGT) 1.18±0.73 U/L, alkaline phosphatase (ALP) 597.30± 231.36 U/L, and creatine kinase (CK) 2348.30±755.60 U/L. Males and females showed significant differences in total proteins, globulins, alkaline phosphatase, A:G ratio, and uric acid.
Abstract in English:
ABSTRACT.- Guimarães J.P., Mari R.B., Miglino M.A., Hernandez-Blasquez F.J. & Watanabe I. 2007. [Mechanoreceptors of the palatine mucosa of ostrich (Struthio camelus): light microscope study.] Mecanoreceptores da mucosa palatina de avestruz (Struthio camelus): estudo ao microscópio de luz. Pesquisa Veterinária Brasileira 27(12):491-494. Departamento de Cirurgia, Setor de Anatomia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270, Brazil. E-mail: juvet@usp.br
Herbst corpuscles of the palatine mucosa of ostrich were studied by light microscopy. The corpuscles are composed of an outer core, inner core and central nerve terminal. The outer core presents numerous lamellae, while the inner core shows compact structure of cytoplasm sheets. The corpuscles are elongate or oval in shape and are surrounded by bundles of collagen fibers. Each lamella is composed of a dense network of thick fibrils. The terminal axons are located along the axis and form a bulb terminal. The fibers of external core stained by Picrosirius and examined by polarized light microscopy revealed to be green in color like type I collagen fibers, and at the periphery is a large amount of collagen type III. The corpuscles are surrounded by flat cells and dense collagen fibers at the periphery.
Abstract in Portuguese:
ABSTRACT.- Guimarães J.P., Mari R.B., Miglino M.A., Hernandez-Blasquez F.J. & Watanabe I. 2007. [Mechanoreceptors of the palatine mucosa of ostrich (Struthio camelus): light microscope study.] Mecanoreceptores da mucosa palatina de avestruz (Struthio camelus): estudo ao microscópio de luz. Pesquisa Veterinária Brasileira 27(12):491-494. Departamento de Cirurgia, Setor de Anatomia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270, Brazil. E-mail: juvet@usp.br
Herbst corpuscles of the palatine mucosa of ostrich were studied by light microscopy. The corpuscles are composed of an outer core, inner core and central nerve terminal. The outer core presents numerous lamellae, while the inner core shows compact structure of cytoplasm sheets. The corpuscles are elongate or oval in shape and are surrounded by bundles of collagen fibers. Each lamella is composed of a dense network of thick fibrils. The terminal axons are located along the axis and form a bulb terminal. The fibers of external core stained by Picrosirius and examined by polarized light microscopy revealed to be green in color like type I collagen fibers, and at the periphery is a large amount of collagen type III. The corpuscles are surrounded by flat cells and dense collagen fibers at the periphery.