Resultado da pesquisa (4)

Termo utilizado na pesquisa Costa E.A

#1 - Babesiosis and anaplasmosis in dairy cattle in Northeastern Brazil, 33(9):1057-1061

Abstract in English:

ABSTRACT.- Souza F.A.L., Braga J.F.V., Pires L.V., Carvalho C.J.S., Costa E.A., Ribeiro M.F.B., Santos R.L. & Silva S.M.M.S. 2013. Babesiosis and anaplasmosis in dairy cattle in Northeastern Brazil. Pesquisa Veterinária Brasileira 33(9):1057-1061. Departamento de Clínica e Cirurgia Veterinária, Centro de Ciências Agrárias, Universidade Federal do Piauí, Av. Universitária s/n, Teresina, PI 64049-550, Brazil. E-mail: chicoleite@hotmail.com The goal of this study was to characterize the epidemiological situation and the factors involved in the prevalence of babesiosis and anaplasmosis in cattle in the dairy basin of Parnaíba, Piauí, Brazil. The study was conducted in 22 farms, and collected blood samples from 202 cattle to study serological, molecular and determination of the packed cell volume (PCV). On the farms were applied surveys involving epidemiological aspects. Seroprevalence rates were: Babesia bigemina 52.5%, B. bovis 68.8%, and Anaplasma marginale 89.1%. Of the samples analyzed, 73.3% were reactive for Babesia spp. and A. marginale, showing co-infection. In PCR, B. bigemina and B. bovis were positive in 52.0% and 33.2% respectively, and A. marginale in 76.2%. Of these, 51.5% amplified DNA of Babesia spp. and A. marginale. The semi-intensive management predominated in 68.0% of the farms studied. The clinical history of babesiosis and anaplasmosis, was reported from 73% of the farms. There was no significant difference (p>0.05) between age groups and for the PCV of positive compared with negative animals. The study indicates that in this region is enzootic instability for babesiosis and enzootic stability for anaplasmosis, reinforcing the fact that in Brazil there are areas of enzootic instability, even in tropical regions of the country. The PCR technique was a valuable tool for the diagnosis of these diseases and may be used to characterize a geographic region.

Abstract in Portuguese:

RESUMO.- Souza F.A.L., Braga J.F.V., Pires L.V., Carvalho C.J.S., Costa E.A., Ribeiro M.F.B., Santos R.L. & Silva S.M.M.S. 2013. Babesiosis and anaplasmosis in dairy cattle in Northeastern Brazil. [Babesiose e anaplasmose em bovinos leiteiros no Nordeste do Brasil.] Pesquisa Veterinária Brasileira 33(9):1057-1061. Departamento de Clínica e Cirurgia Veterinária, Centro de Ciências Agrárias, Universidade Federal do Piauí, Av. Universitária s/n, Teresina, PI 64049-550, Brazil. E-mail: chicoleite@hotmail.com O objetivo deste estudo foi caracterizar a situação epidemiológica e os fatores envolvidos na prevalência da babesiose e anaplasmose em bovinos da bacia leiteira de Parnaíba, Piauí, Brasil. O estudo foi realizado em 22 propriedades, sendo coletadas amostras de sangue de 202 bovinos para estudos sorológicos, moleculares e determinação do volume globular (VG). Nas propriedades foram aplicadas inquéritos envolvendo aspectos epidemiológicos. As taxas de soroprevalência foram: 52,5% para Babesia bigemina, 68,8% B. bovis, e 89,1% para Anaplasma marginale. Das amostras analisadas, 73,3% foram reagentes para Babesia spp. e A. marginale, demostrando co-infecção. Na PCR, B. bigemina e B. bovis foram positivas em 52,0% e 33,2% respectivamente, e A. marginale em 76,2%. Destes, 51,5% amplificaram DNA de Babesia spp. e A. marginale. O manejo semi-intensivo predominou em 68,0% das propriedades estudadas. O histórico clínico de babesiose e anaplasmose foi relatado em 73% das propriedades. Não houve diferença significativa (p>0,05) entre as faixas etárias e para o VG de animais positivos comparados com os negativos. O estudo indica que nesta região há instabilidade enzoótica para babesiose e estabilidade enzoótica para anaplasmose, reforçando o fato de que, no Brasil, existem áreas de instabilidade enzoótica, mesmo em regiões tropicais do país. A técnica de PCR demonstrou ser uma ferramenta valiosa para o diagnóstico destas doenças e pode ser utilizada para caracterizar uma região geográfica.


#2 - Profile of central nervous system samples from cattle with neurological syndrome and diagnosis of bovine rabies by the service of sanitary defense of Minas Gerais, 2003-2010, 32(4):333-339

Abstract in English:

ABSTRACT.- Oliveira T.S., Bull V., Rezende C.A., Furtini R., Costa E.A., Paixão T.A. & Santos R.L. 2012. [Profile of central nervous system samples from cattle with neurological syndrome and diagnosis of bovine rabies by the service of sanitary defense of Minas Gerais, 2003-2010.] Perfil das amostras do sistema nervoso central de bovinos com síndrome neurológica e diagnóstico da raiva bovina no serviço de defesa sanitária de Minas Gerais, 2003-2010. Pesquisa Veterinária Brasileira 32(4):333-339. Setor de Patologia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Cx. Postal 567, Pampulha, Belo Horizonte, MG 30161-970, Brazil. E-mail: rsantos@vet.ufmg.br Diseases of the central nervous system (CNS) are often reported in cattle in Brazil. Although the State of Minas Gerais has the second largest cattle herd in the country, there is little information from this state concerning neurological diseases that affect cattle. The Laboratório de Saúde Animal of the Instituto Mineiro de Agropecuária (LSA/IMA) is in charge of the diagnosis of neurological diseases of livestock in the State, with emphasis on rabies and transmissible spongiform encephalopathies. A retrospective study was conducted on data from cattle with neurologic diseases evaluated by the LSA/IMA from January/2003 to June/2010, aiming to determine the profile of CNS samples sent for analysis, with emphasis on the diagnosis of bovine rabies. Issues related to the animal (sex, age, breed and type of death) as well as to the sample (method of conservation, person in charge the collection, and region of the CNS sampled) were evaluated. Data on frequency of rabies positive samples were analyzed by Fisher’s exact test. During the period studied, 3,731 samples from cattle with neurological signs were analyzed, with a predominance of females and crossbred cattle. The method of preservation was the main problem encountered with only 25.89% of samples sent both refrigerated and fixed in 10% formaldehyde. There was a gradual decrease in submission of samples during the course of this study. All 3,703 samples were evaluated by direct fluorescent antibody test (DFA) and biological test (BT) for rabies, 41.58% being positivity for rabies, and 282 of those samples being subjected to histopathology examination. The frequency of positivity was influenced by breed, age, and type of death. Composition of the sample significantly influenced the results, with higher frequency of positivity in samples containing three or more CNS fragments by DFA, BT, or histopathology. The medulla, which is the fragment of choice for diagnosis of BSE, has often been mistakenly submitted under refrigeration, but not in 10% formalin. Cerebellum, thalamus, brain stem, and spinal cord had higher frequency of Negri bodies than the cerebral cortex and trigeminal ganglia. The nonsuppurative inflammatory infiltrate was less frequent in the cerebral cortex than in other CNS fragments. In conclusion, CNS samples from cattle with neurological syndrome sent to the animal health protection service of Minas Gerais are heterogeneous, and the preservation method was the major problem hindering and adequate diagnosis. In addition, diagnosis of rabies was influenced by parameters of the animal as well as the CNS sample. Submission of properly preserved fragments from various segments of the CNS contributes to a more accurate diagnosis of rabies in cattle.

Abstract in Portuguese:

RESUMO.- Oliveira T.S., Bull V., Rezende C.A., Furtini R., Costa E.A., Paixão T.A. & Santos R.L. 2012. [Profile of central nervous system samples from cattle with neurological syndrome and diagnosis of bovine rabies by the service of sanitary defense of Minas Gerais, 2003-2010.] Perfil das amostras do sistema nervoso central de bovinos com síndrome neurológica e diagnóstico da raiva bovina no serviço de defesa sanitária de Minas Gerais, 2003-2010. Pesquisa Veterinária Brasileira 32(4):333-339. Setor de Patologia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Cx. Postal 567, Pampulha, Belo Horizonte, MG 30161-970, Brazil. E-mail: rsantos@vet.ufmg.br As enfermidades do sistema nervoso central (SNC) são frequentemente relatadas em bovinos no Brasil. Apesar de Minas Gerais ter o segundo maior rebanho bovino do país, há escassez de informações referentes às doenças neurológicas que acometem esses animais. O Laboratório de Saúde Animal do Instituto Mineiro de Agropecuária (LSA/IMA) é o responsável pelo diagnóstico das enfermidades neurológicas dos animais de produção no Estado, com ênfase para a raiva e as encefalopatias espongiformes transmissíveis. Foi realizado um estudo retrospectivo dos dados referentes às amostras de SNC de bovinos com síndrome neurológica avaliadas pelo LSA/IMA de janeiro/2003 a junho/2010, com o objetivo de determinar o perfil das amostras encaminhadas para análise no serviço de defesa sanitária animal, com ênfase no diagnóstico da raiva bovina. Foram consideradas características do animal (sexo, idade, raça e tipo de morte) e da amostra (método de conservação e responsável pela coleta), sendo nas positivas para raiva, avaliada sua composição, assim como as alterações histopatológicas encontradas. Os dados relacionados à frequência de positividade nas diferentes categorias foram submetidos à análise pelo Teste Exato de Fisher. Durante o período avaliado, foram analisadas 3.731 amostras de bovinos com doença neurológica, havendo predomínio de fêmeas e mestiços, o que reflete a composição do rebanho do Estado. O método de conservação foi o principal problema encontrado, sendo apenas 25,89% das amostras encaminhadas em gelo e formol a 10%. Verificou-se uma diminuição gradativa no envio de material para análise. Quanto a raiva bovina diagnosticada no Estado, foram avaliadas 3.703 amostras pela imunofluorescência direta (IFD) e prova biológica (PB), com 41,58% de positividade, sendo dessas 282 submetidas a histopatologia. A frequência de positividade foi influenciada pela raça, idade e tipo de morte do animal. A composição da amostra alterou significativamente o resultado das análises, havendo maior frequência de positividade naquelas compostas por três ou mais fragmentos de SNC, tanto na IFD/PB, quanto na histopatologia. O bulbo, fragmento de eleição para o diagnóstico da EEB, tem sido erroneamente enviado refrigerado e não em formol a 10%. Cerebelo, tálamo, tronco encefálico e medula apresentaram maior frequência de corpúsculos de Negri que cérebro e gânglio trigeminal. O infiltrado inflamatório não supurado foi menos frequente no cérebro, que nos demais fragmentos avaliados. Conclui-se que as amostras de bovinos com síndrome neurológica enviadas ao serviço de defesa sanitária animal de Minas Gerais apresentam características distintas, sendo o método de conservação o principal problema encontrado. Além disso, a raiva bovina diagnosticada na população estudada é influenciada pelas características do animal e da amostra, sendo indicado o envio de diferentes fragmentos do SNC para análise, conservados adequadamente, o que contribui para um diagnóstico mais preciso.


#3 - Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil, 31(10):859-866

Abstract in English:

ABSTRACT.- Mineiro A.L.B.B., Vieira R.J., Costa E.A., Santos R.L., Gonçalves L.M.F., Carvalho S.M., Bomfim M.R.Q. & Costa F.A.L. 2011. Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil. Pesquisa Veterinária Brasileira 31(10):859-866. Departamento de Clínica e Cirurgia Veterinária, Centro de Ciências Agrárias, Universidade Federal do Piauí, Campus da Socopo, Bairro Ininga, Teresina, PI 64049-550, Brazil. E-mail: fassisle@gmail.com The presence of anti leptospiral agglutinins (microscopic agglutination test - MAT) and DNA of leptospires was investigated in the kidney and urine (Polymerase Chain Reaction - PCR) in samples collected at the time of slaughter of cattle originating from the dairy basin of Parnaíba, Piauí, Brazil, as also the lesions in kidney, lung, liver, uterus, ovary and placenta (histopathology and immunohistochemistry). In the MAT, Hardjo was the predominant serovar with the highest number of reagent animals for the strain Hardjobovis/Sponselee. Anti-leptospiral antigens were scored in epithelial cells, interstitial vascular endothelium, endothelium of glomerular capillaries and Bowman’s capsule of 20 positive animals. Inflammatory cells were more common in the kidney. PCR was positive in urine and kidney tissue.

Abstract in Portuguese:

RESUMO.- Mineiro A.L.B.B., Vieira R.J., Costa E.A., Santos R.L., Gonçalves L.M.F., Carvalho S.M., Bomfim M.R.Q. & Costa F.A.L. 2011. Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil. [Sorologia, reação em cadeia da polimerase e histopatologia para leptospirose em amostras coletadas de vacas leiteiras, em matadouro da região de Parnaíba, Piauí.] Pesquisa Veterinária Brasileira 31(10):859-866. Departamento de Clínica e Cirurgia Veterinária, Centro de Ciências Agrárias, Universidade Federal do Piauí, Campus da Socopo, Bairro Ininga, Teresina, PI 64049-550, Brazil. E-mail: fassisle@gmail.com Foi investigada a presença de aglutininas anti leptospiras (reação de soroaglutinação microscópica - SAM), de DNA de leptospiras no rim e na urina (reação de cadeia pela polimerase - PCR), bem como de lesões no rim, pulmão, fígado, útero, ovário e placenta (histopatologia e imunohistoquímica), em materiais colhidos, por ocasião do abate, de bovinos originários da bacia leiteira da região de Parnaíba, Piauí, Brasil. Na SAM o sorovar predominante foi o Hardjo com maior número de animais reagentes para a estirpe Hardjobovis/Sponselee. Antígenos anti leptospira foram marcados em 20 animais positivos nas células epiteliais e do endotélio vascular, endotélio dos capilares glomerulares e na cápsula de Bowman, somente nos animais infectados. O infiltrado inflamatório foi maior no rim do que nos demais órgãos. A PCR foi positiva em amostras de urina e tecido renal.


#4 - An outbreak of malignant catarrhal fever in Murrah buffaloes in Minas Gerais, Brazil, p.395-400

Abstract in English:

ABSTRACT.- Costa E.A., Bastianetto E., Vasconcelos A.C., Bomfim M.R.Q., Fonseca F.G., Gomes A.D., Leite R.C. & Resende M. 2009. An outbreak of malignant catarrhal fever in Murrah buffaloes in Minas Gerais, Brazil. Pesquisa Veterinária Brasileira 29(5):395-400. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil. E-mail: lvc@icb.ufmg.br An outbreak of Malignant Catarrhal Fever (MCF) resulted in death of five female buffaloes and one domestic cow from the same farm. Four buffaloes died 10-15 days after the appearance of clinical signs, while the fifth was euthanized in extremis, after similar clinical signs. Histopathological lesions included multifocal histiolymphocytic epicarditis, myocarditis and lymphocytic interstitial pneumonia, which are commonly seen in cases of MCF in buffaloes. Furthermore, lymphocytic vasculitis centered in the adventitia, with occasional fibrinoid necrosis in the muscular layer, was found in the kidneys, liver, spleen, lymph nodes and brain. Nucleotide sequencing of DNA fragments from the central nervous system amplified by PCR revealed 98% similarity with known OHV-2 sequences from Genbank. Additionally, PCR analysis also revealed the presence of OHV-2 DNA in the peripheral mononuclear blood cells of two clinically healthy buffaloes. The diagnosis of MCF was based on epidemiological, clinical, gross and histopathological findings and on the results of a semi-nested PCR followed by nucleotide sequencing.

Abstract in Portuguese:

ABSTRACT.- Costa E.A., Bastianetto E., Vasconcelos A.C., Bomfim M.R.Q., Fonseca F.G., Gomes A.D., Leite R.C. & Resende M. 2009. An outbreak of malignant catarrhal fever in Murrah buffaloes in Minas Gerais, Brazil. Pesquisa Veterinária Brasileira 29(5):395-400. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil. E-mail: lvc@icb.ufmg.br An outbreak of Malignant Catarrhal Fever (MCF) resulted in death of five female buffaloes and one domestic cow from the same farm. Four buffaloes died 10-15 days after the appearance of clinical signs, while the fifth was euthanized in extremis, after similar clinical signs. Histopathological lesions included multifocal histiolymphocytic epicarditis, myocarditis and lymphocytic interstitial pneumonia, which are commonly seen in cases of MCF in buffaloes. Furthermore, lymphocytic vasculitis centered in the adventitia, with occasional fibrinoid necrosis in the muscular layer, was found in the kidneys, liver, spleen, lymph nodes and brain. Nucleotide sequencing of DNA fragments from the central nervous system amplified by PCR revealed 98% similarity with known OHV-2 sequences from Genbank. Additionally, PCR analysis also revealed the presence of OHV-2 DNA in the peripheral mononuclear blood cells of two clinically healthy buffaloes. The diagnosis of MCF was based on epidemiological, clinical, gross and histopathological findings and on the results of a semi-nested PCR followed by nucleotide sequencing.


Colégio Brasileiro de Patologia Animal SciELO Brasil CAPES CNPQ UNB UFRRJ CFMV