Resultado da pesquisa (7)

Termo utilizado na pesquisa Masuda E.K

#1 - Epidemiological, clinical and pathological features of canine parvovirus 2c infection in dogs from southern Brazil

Abstract in English:

Canine parvovirus type 2c (CPV-2c) emerged in Europe in the early 2000’s and rapidly spread out worldwide. Clinical and molecular data have demonstrated its circulation in Brazilian dogs, yet detailed descriptions of cases are still lacking. This article describes the epidemiological, clinical and pathological features of 24 cases of CPV-2c-associated disease in dogs submitted to veterinary clinics and laboratory diagnosis in southern Brazil (2014-2016). Most affected dogs presented signs/lesions suggestive of parvovirus enteritis: diarrhea, vomiting, hyperemia and hemorrhage of the serous membrane of the small intestine, diffuse segmental granulation, atrophy of the villi, necrosis and fusion of crypts, squamous metaplasia and epithelial syncytia. A number of cases presented features divergent from the classical presentations, including a wide variation in the color of feces (reddish and/or yellowish, light-brownish, orange-brown and brownish), involvement of adults (4/24) and vaccinated dogs (12/24), extensive involvement of the small intestine (8/20) and the presence of pulmonary edema (7/24) and convulsions (3/24). Feces and intestinal fragments submitted to PCR for the CPV-2 VP2 gene and to virus isolation in cell culture yielded positive results in 100% and 58.3% (14/24) of the cases, respectively. Nucleotide sequencing revealed a high nucleotide identity in VP2 (99.4 to 100%) and a consistent mutation at amino acid 426 (asparagine to glutamic acid), considered a signature of CPV-2c. These results confirm the involvement of CPV-2c in the described cases and demonstrate the importance of CPV-2c infection among Brazilian dogs, calling attention of veterinarians to correctly diagnose the disease, mainly considering the frequent atypical presentations.

Abstract in Portuguese:

O parvovírus canino tipo 2c (CPV-2c) surgiu na Europa no início do ano 2000 e rapidamente se espalhou pelas populações de cães ao redor do mundo. Dados clínicos e moleculares demonstraram a sua circulação em cães brasileiros, porém descrições detalhadas desses casos ainda são escassas. Este artigo descreve os aspectos epidemiológicos, clínicos e patológicos de 24 casos de doença gastroentérica associada com a infecção pelo CPV-2c em cães atendidos em clínicas veterinárias e submetidos ao diagnóstico laboratorial no Sul do Brasil (2014-2016). A maioria dos cães afetados apresentaram sinais e/ou lesões sugestivas de enterite por parvovírus: diarreia, vômitos, hiperemia e hemorragia na membrana serosa do intestino delgado, granulação segmentar difusa, atrofia das vilosidades, necrose e fusão de criptas, metaplasia escamosa e sincícios epiteliais. Alguns casos apresentaram características divergentes das apresentações clássicas, incluindo uma grande variação na cor das fezes (avermelhada e/ou amarelada, marrom-claro, marrom-alaranjada ou amarronzada), a participação dos adultos (4/24) e cães vacinados (12/24), um amplo envolvimento do intestino delgado (8/20), a presença de edema pulmonar (7/24) e convulsões (3/24). As fezes e fragmentos intestinais foram submetidos ao teste de PCR para o gene VP2 do CPV-2, e ao isolamento do vírus em cultura de células produziram resultados positivos em 100% e 58,3% (14/24) dos casos, respectivamente. O sequenciamento dos nucleótidos revelou uma alta identidade de nucleótidos na VP2 (99,4-100%) e uma mutação no aminoácido 426 (asparagina para ácido glutâmico), considerada uma assinatura de CPV-2c. Estes resultados confirmam o envolvimento do CPV-2c nos casos descritos e demonstra a importância da infecção pelo CPV-2c entre os cães do Brasil, chamando a atenção de veterinários para diagnosticar corretamente a doença, principalmente considerando-se as apresentações atípicas frequentes.


#2 - Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil, 35(6):557-561

Abstract in English:

ABSTRACT.- Cargnelutti J.F., Masuda E.K., Neuls M.G., Weiblen R. & Flores E.F. 2015. Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil. Pesquisa Veterinária Brasileira 35(6):557-561. Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil. E-mail: eduardofurtadoflores@gmail.com Canid herpesvirus 1 (CHV-1) is a widespread pathogen of dogs and produces infertility, abortions and severe systemic disease in young puppies. Clinical data indicate the circulation of CHV-1 among Brazilian dogs yet definitive diagnosis has rarely been accomplished. This article describes the clinicopathological findings of four independent cases/outbreaks of neonatal disease by CHV-1 in Bulldog puppies followed by virus identification and genetic characterization. Three events occurred in a kennel holding dogs of different breeds at reproductive age (March 2013, October 2013 and April 2014). Puppies from three French or English Bulldog litters, aging 9 to 30 days were affected, presenting dyspnea, agonic breathing, pale mucous, abdominal pain and tension, evolving to death within about 24 hours. At necropsy, the puppies presented necrohemorrhagic hepatitis, multifocal and moderate necrohemorrhagic nephritis and fibrinonecrotic interstitial pneumonia. Virus isolation was positive in clinical specimens from one litter and CHV-1 DNA was detected by PCR in tissues from all four cases. Virus-neutralizing assays with samples of the affected kennel revealed 9/12 adult animals with high antibody titers to CHV-1. Nucleotide sequencing of glycoprotein B, C and D genes revealed 99-100% of identity among the viruses and with CHV-1 sequences available in GenBank. Phylogenetic analyses of gC sequences showed a segregation of the samples, even among three isolates from the same kennel. These findings support CHV-1 infection as the cause of disease and death in these dog litters, reinforcing the need for correct etiologic diagnosis, prevention and immunization against CHV-1 in dogs from Southern Brazil.

Abstract in Portuguese:

RESUMO.- Cargnelutti J.F., Masuda E.K., Neuls M.G., Weiblen R. & Flores E.F. 2015. Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil. [Surtos de doença por herpesvírus canino 1 em filhotes de cães no sul do Brasil.] Pesquisa Veterinária Brasileira 35(6):557-561. Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil. E-mail: eduardofurtadoflores@gmail.com O herpesvírus canino (CHV-1) é um patógeno de cães que possui distribuição mundial e que causa infertilidade, abortos e doença sistêmica severa em filhotes de cães. Achados clínicos tem indicado a circulação do CHV-1 em cães no Brasil, embora o diagnóstico definitivo seja raramente determinado. Este artigo descreve os achados clinicopatológicos de quatro casos/surtos independentes de morte neonatal de filhotes de cães da raça Bulldog causados pelo CHV-1, a identificação e a caracterização genética do vírus. Três eventos ocorreram no mesmo canil que abriga animais de diferentes raças em idade reprodutiva (março de 2013, outubro de 2013 e abril de 2014). Filhotes de três ninhadas de Bulldog Francês e/ou Inglês, com idade de 9 a 30 dias, foram afetados e apresentaram dispneia, respiração agônica, mucosas pálidas, dor e tensão abdominal, que evoluíram para morte dos cães dentro de, aproximadamente, 24 horas. Na necropsia foram observados hepatite necro-hemorrágica, nefrite necro-hemorrágica multifocal e moderada e pneumonia intersticial fibrinonecrótica. O isolamento viral foi positivo em amostras clínicas de um filhote e DNA de CHV-1 foi detectado por PCR em tecidos de filhotes de todos os surtos. Teste de soroneutralização com amostras de soro de cães provenientes do canil afetado revelaram que nove de 12 animais adultos possuíam altos títulos de anticorpos para o CHV-1. Sequenciamento de nucleotídeos do gene das glicoproteínas B, C e D revelaram 99-100% de identidade entre as amostras e com as sequências de CHV-1 disponíveis no GenBank. A análise filogenética baseada na sequência do gene da glicoproteína C mostrou uma segregação das amostras, mesmo entre os três isolados de vírus provenientes do mesmo canil. Esses achados demonstram que o CHV-1 é a causa da doença e da morte dos filhotes, reforçando a necessidade do correto diagnóstico etiológico e a implementação de medidas de prevenção e imunização contra o CHV-1 em cães no sul do Brasil.


#3 - A retrospective study of 207 cases of mammary tumors in queens,

Abstract in English:

ABSTRACT.- Togni M., Masuda E.K., Kommers G.D., Fighera R.A. & Irigoyen L.F. 2013. [A retrospective study of 207 cases of mammary tumors in queens.] Estudo retrospectivo de 207 casos de tumores mamários em gatas. Pesquisa Veterinária Brasileira 33(3):353-358. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: moniquetogni@yahoo.com.br This study aimed to determine the most prevalent mammary tumors in cats and associate them to some prognostic factors. The files from the Laboratório de Patologia Veterinária (LPV) of the Universidade Federal de Santa Maria (UFSM) were reviewed, and 1.427 feline biopsies and autopsy protocols between the years 2000 and 2011 were found. Based on the information retrieved from the files, a relationship was established among the tumors and some prognostic factors such as sex, age, breed, reproductive status, use of contraceptives, number and location of affected glands, ulcers, size of the neoplasm, distant metastases, and affected lymph nodes. Thus, it was observed that mammary cancer is the second most prevalent diagnosis, following skin tumors. All cats with mammary tumors were female, being the elderly and mixed breed the most affected. Malignant neoplasms were the most frequently diagnosed, followed by non-neoplastic tumors, and benign neoplasms. Smaller tumors were mostly carcinomas. Ulcerations were present not only in malignant neoplasms but also in non-neoplastic changes. Distant metastases were found mainly to the lungs and skin.

Abstract in Portuguese:

RESUMO.- Togni M., Masuda E.K., Kommers G.D., Fighera R.A. & Irigoyen L.F. 2013. [A retrospective study of 207 cases of mammary tumors in queens.] Estudo retrospectivo de 207 casos de tumores mamários em gatas. Pesquisa Veterinária Brasileira 33(3):353-358. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: moniquetogni@yahoo.com.br Este estudo teve como objetivos determinar os tumores mais prevalentes em gatos e relacionar os tumores mamários a alguns de seus fatores prognósticos. Os arquivos do Laboratório de Patologia Veterinária (LPV) da Universidade Federal de Santa Maria (UFSM) foram revisados e um total de 1.427 protocolos de biopsias e necropsias de felinos, entre 2000 e 2011, foi encontrado. Com base nas informações dos arquivos, foi estabelecida a relação entre os tumores e alguns fatores como sexo, idade, raça, estado reprodutivo, uso de contraceptivos, número e localização das glândulas afetadas, ulcerações, tamanho do neoplasma, metástases distantes e para os linfonodos. Assim, observou-se que os tumores de mama foram o segundo diagnóstico mais prevalente, após os tumores de pele. Todos os gatos com tumores mamários eram fêmeas, sendo os sem raça definida e os idosos os mais afetados. Os neoplasmas malignos foram diagnosticados com maior frequência, seguidos pelos tumores não neoplásicos e pelos neoplasmas benignos. Os tumores menores eram, na sua maioria, carcinomas. Ulcerações estavam presentes não só em neoplasmas malignos, mas também em alterações não neoplásicas. Metástases distantes foram encontradas principalmente nos pulmões e na pele.


#4 - Relationship between lymphopenia and the persistence of alimentary papillomatosis in cattle chronically and spontaneously poisoned by bracken fern (Pteridium aquilinum), 31(5):383-388

Abstract in English:

ABSTRACT.- Masuda E.K., Kommers G.D., Rosa F.B., Barros C.S.L., Fighera R.A. & Piazer J.V.M. 2011. [Relationship between lymphopenia and the persistence of alimentary papillomatosis in cattle chronically and spontaneously poisoned by bracken fern (Pteridium aquilinum).] Relação entre a linfopenia e a persistência da papilomatose alimentar em bovinos intoxicados crônica e espontaneamente por samambaia (Pteridium aquilinum). Pesquisa Veterinária Brasileira 31(5):383-388. Laboratório de Patologia Veterinária, Departamento de Patologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, RS 97105-900, Brazil. E-mail:glaukommers@yahoo.com Bovine papillomavirus type-4 (BPV-4) is responsible for the development of papillomas in the upper digestive tract (UDT) of cattle. The necessary steps for the development, growth and regression of papillomas are intimately related with the immunological status of the animal. The consumption of bracken fern (Pteridium aquilinum) has been pointed out as the major factor involved in the persistence of BPV-4 infection of the UDT. The theory involving the relationship between alimentary papillomas and the formation of squamous cell carcinomas (SCCs) suggests the persistence of alimentary papillomatosis through a chronic immunosupressive lymphopenic state induced by bracken fern. The papillomas would serve then as a development site of SCCs through the interaction of BPV-4 oncoproteins and bracken carcinogens. The objective of this study was to evaluate the relationship between lymphopenia and alimentary papillomatosis in spontaneous cases of chronic bracken fern poisoning of cattle. The age, intensity of papillomatosis and the leukon were evaluated of 40 cattle with SCCs of the UDT. Three animals had leukopenia and one had neutrophilia. The mean lymphocyte count (and standart deviation) of mild, moderate and severe papillomatosis was 5,395(±1,696)/mm3, 4,560(±1,561)/mm3 and 5,007(±1,786)/mm3, respectively. There were no statistically significant differences between the intensity of alimentary papillomasis, age and the lymphocyte count. Immunosupression by lymphopenia was a sporadic finding (three cases) in the present study. The results demonstrate that the persistence of alimentary papilomatosis in spontaneous cases of bracken fern-related SCCs of the UDT is not related to lymphopenia and it may be related to other immunological factors.

Abstract in Portuguese:

RESUMO.- Masuda E.K., Kommers G.D., Rosa F.B., Barros C.S.L., Fighera R.A. & Piazer J.V.M. 2011. [Relationship between lymphopenia and the persistence of alimentary papillomatosis in cattle chronically and spontaneously poisoned by bracken fern (Pteridium aquilinum).] Relação entre a linfopenia e a persistência da papilomatose alimentar em bovinos intoxicados crônica e espontaneamente por samambaia (Pteridium aquilinum). Pesquisa Veterinária Brasileira 31(5):383-388. Laboratório de Patologia Veterinária, Departamento de Patologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Avenida Roraima 1000, Santa Maria, RS 97105-900, Brazil. E-mail:glaukommers@yahoo.com Papilomavírus bovino tipo 4 (BPV-4) é responsável pelo desenvolvimento de papilomas no trato alimentar superior (TAS) de bovinos. Os passos necessários para o crescimento, o desenvolvimento e a regressão dos papilomas estão intimamente relacionados com o estado imunológico do animal. A ingestão de samambaia (Pteridium aquilinum) tem sido relacionada como o principal fator envolvido na persistência da infecção pelo BPV-4 no TAS. A teoria que estabelece a relação entre papilomatose alimentar e a formação de CCEs sugere a produção de um estado imunossupressivo crônico pela planta, permitindo a persistência dos papilomas no TAS. Os papilomas serviriam então como sítios de desenvolvimento dos CCEs através da interação entre as proteínas do BPV-4 e os carcinógenos da samambaia. O objetivo deste estudo foi avaliar a relação entre a quantidade de linfócitos circulantes e a papilomatose alimentar em casos de intoxicação espontânea crônica por P. aquilinum em bovinos com CCE no TAS. Quarenta bovinos com CCEs no TAS foram avaliados quanto à idade, à intensidade da papilomatose alimentar no TAS e ao leucograma. Três bovinos tinham leucopenia e um apresentava neutrofilia. A média de linfócitos foi de 5.395 (±1.696) na papilomatose leve, 4.560 (±1.561) na moderada e 5.007 (±1.786) na acentuada. Não houve diferença estatisticamente significativa entre o grau de papilomatose, a idade e a quantidade de linfócitos circulantes. Imunossupressão por linfopenia foi um achado esporádico (três casos) neste estudo. Os resultados indicam que a persistência da papilomatose alimentar em casos espontâneos de intoxicação crônica por samambaia em bovinos não tem relação com a quantidade de linfócitos circulantes e que talvez esteja relacionada a outros fatores imunológicos.


#5 - Retrospective study of 1,647 mammary gland tumors in dogs, 30(2):177-185

Abstract in English:

ABSTRACT.- Oliveira Filho J.C., Kommers G.D., Masuda E.K., Marques B.M.F.P.P., Fighera R.A., Irigoyen L.F. & Barros C.S.L. 2010. [Retrospective study of 1,647 mammary gland tumors in dogs.] Estudo retrospectivo de 1.647 tumores mamários em cães. Pesquisa Veterinária Brasileira 30(2):177-185. Departamento de Patologia, Universidade Federal de Santa Maria, Av. Roraima 1000, Camobi, 97105-900 Santa Maria, RS, Brazil. E-mail: glaukommers@yahoo.com Mammary gland tumors are common in dogs and are the most common type of neoplasm of female dogs. The main purposes of this study were to establish the prevalence of the mammary gland tumors, as well as of its different histologic types (neoplastic and non-neoplastic), diagnosed in the Laboratório de Patologia Veterinária of the Universidade Federal de Santa Maria. There were 1,304 biopsy reports of mammary gland tumors during 1990-2008 and 132 necropsy reports of dogs with mammary tumors in 2000-2008. Data about breed, gender, and age of dogs, tumor localization, morphologic diagnosis, and metastasis occurrence were analyzed. In the biopsy cases, 1,495 tumors were diagnosed, considering the presence of more than one type of tumor in some of the dogs. From those, 1,465 were neoplastic and 30 were non-neoplastic. Within the neoplasms, 390 (26.6%) were benign and 1,075 (73.3%) were malignant. The benign/malignant ratio was 1:2.75. During the years 2000-2008, 1,125 necropsies of dogs were performed, 132 (11.64%) of which with mammary gland tumors. There were 152 morphologic diagnoses, being 150 of neoplastic and two of non-neoplastic lesions. Simple carcinoma was the most prevalent histologic type, both among biopsy and necropsy cases. On necropsy cases, metastases to lymph nodes and distant organs were seen in 39 (29.5%) and 64 (53,3%) cases, respectively.

Abstract in Portuguese:

RESUMO.- Oliveira Filho J.C., Kommers G.D., Masuda E.K., Marques B.M.F.P.P., Fighera R.A., Irigoyen L.F. & Barros C.S.L. 2010. [Retrospective study of 1,647 mammary gland tumors in dogs.] Estudo retrospectivo de 1.647 tumores mamários em cães. Pesquisa Veterinária Brasileira 30(2):177-185. Departamento de Patologia, Universidade Federal de Santa Maria, Av. Roraima 1000, Camobi, 97105-900 Santa Maria, RS, Brazil. E-mail: glaukommers@yahoo.com Tumores mamários são comuns em cães, e constituem o neoplasma mais frequente em cadelas. Este estudo tem como objetivos principais estabelecer a prevalência dos tumores mamários, e dos diferentes tipos histológicos (neoplásicos e não-neoplásicos), diagnosticados em cães no Laboratório de Patologia Veterinária da Universidade Federal de Santa Maria (LPV-UFSM). Foram avaliados 1.304 protocolos de exames histopatológicos recebidos em 1990-2008 e 132 protocolos de necropsias realizadas em 2000-2008. Deles foram obtidos a raça, o sexo e a idade dos cães, a localização do tumor na cadeia mamária, o diagnóstico morfológico e a ocorrência de metástases. Nos protocolos de exame histopatológico, foram diagnosticados 1.495 tumores, considerando que alguns cães tinham mais de um tipo de tumor, sendo 1.465 neoplásicos e 30 não-neoplásicos. Dos neoplásicos, 390 (26,6%) eram benignos e 1.075 (73,3%) eram malignos, estabelecendo-se uma relação benigno: maligno de 1:2,75. Das 1.125 necropsias de cães realizadas em 2000-2008, 132 (11,6%) protocolos tinham descrições de tumor de mama, perfazendo 152 diagnósticos morfológicos, dos quais 150 eram neoplásicos e dois eram não-neoplásicos. Carcinoma simples foi o tipo histológico mais prevalente tanto em biópsias como em necropsias. Nas necropsias, foram observadas metástases para linfonodos em 39 casos (29,5%) e para órgãos distantes em 64 casos (53,3%).


#6 - Clinical, morphologic and immunohistochemical aspects of canine gastrintestinal pythiosis, 29(8):673-679

Abstract in English:

ABSTRACT.- Trost M.E., Gabriel A.L., Masuda E.K., Fighera R.A., Irigoyen L.F. & Kommers G.D. 2009. [Clinical, morphologic and immunohistochemical aspects of canine gastrintestinal pythiosis.] Aspectos clínicos, morfológicos e imuno-histoquímicos da pitiose gastrintestinal canina. Pesquisa Veterinária Brasileira 29(8):673-679. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: glaukommers@yahoo.com Four suspect and two confirmed cases of gastrointestinal pythiosis were found in a retrospective study of biopsy and necropsy cases of dogs received in the Laboratório de Patologia Veterinária at the Universidade Federal de Santa Maria. The two diagnosed and published cases have had the etiology confirmed by culture and zoosporogenesis induction or by nested-PCR. On this study, the etiologic diagnosis of four suspect cases was confirmed by immunohistochemistry using anti-Pythium insidiosum policlonal antibody. Epidemiology, clinical signs, gross and microscopic lesions, histochemistry, immunohistochemistry, and differential diagnoses are reported and discussed.

Abstract in Portuguese:

RESUMO.- Trost M.E., Gabriel A.L., Masuda E.K., Fighera R.A., Irigoyen L.F. & Kommers G.D. 2009. [Clinical, morphologic and immunohistochemical aspects of canine gastrintestinal pythiosis.] Aspectos clínicos, morfológicos e imuno-histoquímicos da pitiose gastrintestinal canina. Pesquisa Veterinária Brasileira 29(8):673-679. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: glaukommers@yahoo.com Através de um estudo retrospectivo dos casos de biópsias e necropsias de cães recebidos no Laboratório de Patologia Veterinária da Universidade Federal de Santa Maria, constatou-se a ocorrência de dois casos confirmados e de quatro casos suspeitos de pitiose gastrintestinal canina. Os dois casos diagnosticados e publicados tiveram a etiologia confirmada através da cultura e indução de zoosporogênese ou por nested-PCR. Neste estudo utilizou-se a técnica de imuno-histoquímica com anticorpo policlonal anti-Pythium insidiosum para confirmação da etiologia dos quatro casos suspeitos. A epidemiologia, sinais clínicos, lesões macroscópicas e microscópicas, características histoquímicas e imuno-histoquímicas e diagnósticos diferenciais são relatados e discutidos.


#7 - Aspectos clínicos-hematológicos e lesões vesicais nas formas crônicas de intoxicação espontânea por Pteridium aquilinum em bovinos, p.515-525

Abstract in English:

ABSTRACT.- Gabriel A.L., Kommers G.D., Masuda E.K, Fighera R.A., Piazer J.V.M., Barros C.S.L., Martins T.B. & Rosa F.B. 2009. [Clinical and hematological aspects and urinary bladder lesions in chronic spontaneous poisoning by Pteridium aquilinum in cattle.] Aspectos clínicos-hematológicos e lesões vesicais nas formas crônicas de intoxicação espontânea por Pteridium aquilinum em bovinos. Pesquisa Veterinária Brasileira 29(7):515-525. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: glaukommers@yahoo.com Spontaneous cases of chronic poisoning by Pteridium aquilinum in cattle were studied. The clinical forms of the disease were squamous cell carcinoma (SCCs) of the upper digestive tract (UDT) and bovine enzootic hematuria (BEH). The cases were from the midland Region of the Midwest of Rio Grande do Sul State, Brazil, and were submitted to the Laboratório de Patologia Veterinária of the Universidade Federal de Santa Maria. Clinical signs and blood work were evaluated at terminal phase of disease. Cattle with UDT SCCs had progressive weigth loss, ruminal atony, cough, dysphagia, bloating, and regurgitation. In cattle with BEH, hematuria was observed in all cases, followed by progressive weight loss. Non-regenerative anemia was detected in 33.33% of the cattle with UDT SCCs form and in 66.66% of the cattle with BEH form. Changes in white blood count occurred in some cases but drop in lymphocyte numbers was uncommon in both forms of disease. For the morphological study, urinary bladders from 46 cattle with UDT SCCs and 11 cattle with BEH were analyzed. Grossly, 16/46 bladders from the UDT SCCs form had gross lesions (red or pale vesical nodules, hemorrhages, and papilomas; red urine was detected at necropsy of only three cases). In BEH form, the bladder had nodules, large neoplastic masses, red urine, papilomas, and hemorrhages. Pyelonephritis and hydronephrosis were seen in a few cases. Microscopically, in the UDT SCCs form, 44/46 (95.65%) bladders had 22 different types of morphological changes, characterized by neoplastic lesions (5/22) and non-neoplastic lesions (17/22); the latter were subdivided in non-neoplastic epithelial changes (6/17), general changes of the lamina propria (6/17), and inflammatory changes (5/17). The bladder changes in BEH form were of 19 different types, characterized by neoplastic lesions (5/19) and non-neoplastic lesions (14/19), which were subdivided in non-neoplastic epitelial changes (9/14), general changes of the lamina propria (3/14), and inflammatory changes (2/14). In BEH, mesenchymal neoplasms were more observed than epithelial ones, and most of them were malignant. Immunohistochemistry was utilized to characterize the histogenesis of poorly differentiated neoplasms. In conclusion, the morphological study demonstrated that urinary bladder lesions identical to the those seen in cattle with BEH, are very often present in cattle with the UDT SCCs form.

Abstract in Portuguese:

ABSTRACT.- Gabriel A.L., Kommers G.D., Masuda E.K, Fighera R.A., Piazer J.V.M., Barros C.S.L., Martins T.B. & Rosa F.B. 2009. [Clinical and hematological aspects and urinary bladder lesions in chronic spontaneous poisoning by Pteridium aquilinum in cattle.] Aspectos clínicos-hematológicos e lesões vesicais nas formas crônicas de intoxicação espontânea por Pteridium aquilinum em bovinos. Pesquisa Veterinária Brasileira 29(7):515-525. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: glaukommers@yahoo.com Spontaneous cases of chronic poisoning by Pteridium aquilinum in cattle were studied. The clinical forms of the disease were squamous cell carcinoma (SCCs) of the upper digestive tract (UDT) and bovine enzootic hematuria (BEH). The cases were from the midland Region of the Midwest of Rio Grande do Sul State, Brazil, and were submitted to the Laboratório de Patologia Veterinária of the Universidade Federal de Santa Maria. Clinical signs and blood work were evaluated at terminal phase of disease. Cattle with UDT SCCs had progressive weigth loss, ruminal atony, cough, dysphagia, bloating, and regurgitation. In cattle with BEH, hematuria was observed in all cases, followed by progressive weight loss. Non-regenerative anemia was detected in 33.33% of the cattle with UDT SCCs form and in 66.66% of the cattle with BEH form. Changes in white blood count occurred in some cases but drop in lymphocyte numbers was uncommon in both forms of disease. For the morphological study, urinary bladders from 46 cattle with UDT SCCs and 11 cattle with BEH were analyzed. Grossly, 16/46 bladders from the UDT SCCs form had gross lesions (red or pale vesical nodules, hemorrhages, and papilomas; red urine was detected at necropsy of only three cases). In BEH form, the bladder had nodules, large neoplastic masses, red urine, papilomas, and hemorrhages. Pyelonephritis and hydronephrosis were seen in a few cases. Microscopically, in the UDT SCCs form, 44/46 (95.65%) bladders had 22 different types of morphological changes, characterized by neoplastic lesions (5/22) and non-neoplastic lesions (17/22); the latter were subdivided in non-neoplastic epithelial changes (6/17), general changes of the lamina propria (6/17), and inflammatory changes (5/17). The bladder changes in BEH form were of 19 different types, characterized by neoplastic lesions (5/19) and non-neoplastic lesions (14/19), which were subdivided in non-neoplastic epitelial changes (9/14), general changes of the lamina propria (3/14), and inflammatory changes (2/14). In BEH, mesenchymal neoplasms were more observed than epithelial ones, and most of them were malignant. Immunohistochemistry was utilized to characterize the histogenesis of poorly differentiated neoplasms. In conclusion, the morphological study demonstrated that urinary bladder lesions identical to the those seen in cattle with BEH, are very often present in cattle with the UDT SCCs form.


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