Resultado da pesquisa (5)

Termo utilizado na pesquisa Uzal F.A

#1 - Blackleg in a free-range brown brocket deer (Mazama gouazoubira)

Abstract in English:

A case of blackleg in a brown brocket deer (Mazama gouazoubira) associated with trauma from being hit by a car in southern Rio Grande do Sul is reported. The clinical signs included fever, dehydration and lethargy that worsened progressively until 36 hours after the accident, when the animal died. In the fore right limb, there was a comminuted closed fracture of the radius and ulna but no skin wounds were observed. Grossly, the musculature of the pelvic limbs presented hemorrhage, edema and emphysema. Microscopically, the muscles of both rear legs had necrosis, edema, hemorrhage and mild inflammatory infiltration of neutrophils. Clostridium chauvoei was cultured from affected skeletal muscles, and it was also detected by immunohistochemistry, confirming a diagnosis of blackleg. The overlapping habitat of cattle and brown brocket deer is proposed as a predisposing factor in this case and alerts to spillover cases maybe happening in this region. In addition, blackleg should be included as differential diagnoses of deer with post-traumatic myositis.

Abstract in Portuguese:

Descreve-se um caso de carbúnculo sintomático em um veado-virá (Mazama gouazoubira), macho, jovem, resgatado após atropelamento em uma rodovia na região sul do Rio Grande do Sul. O cervídeo apresentou febre, desidratação e letargia, progredindo para a morte em 36 horas. No membro torácico direito foi observado fratura cominutiva fechada de rádio e ulna sem a presença de feridas perfurantes. Na necropsia foi observada hemorragia, edema e enfisema na musculatura dos membros pélvicos. Microscopicamente, os músculos dos membros pélvicos apresentaram necrose, edema, hemorragia e discreto infiltrado inflamatório neutrofílico. Houve o isolamento de Clostridium chauvoei e marcação positiva na técnica de IHQ com anticorpo monoclonal anti-C. chauvoei, confirmando o diagnóstico de carbúnculo sintomático. A sobreposição de habitat entre bovinos domésticos e cervídeos pode ser um fator de risco para esta doença e chama a atenção para casos de “spillover” que podem estar ocorrendo na região. Adicionalmente, sugere-se que o carbúnculo sintomático seja incluído nos diagnósticos diferenciais de cervídeos que apresentam miosite pós-traumática.


#2 - Equine leukoencephalomalacia (ELEM) due to fumonisins B1 and B2 in Argentina, 31(5):407-412

Abstract in English:

ABSTRACT.- Giannitti F., Diab S.S., Pacin A.M., Barrandeguy M., Larrere C., Ortega J. & Uzal F.A. 2011. Equine leukoencephalomalacia (ELEM) due to fumonisins B1 and B2 in Argentina. Pesquisa Veterinária Brasileira 31(5):407-412. Laboratorio de Diagnóstico Veterinario, calle 25 de Mayo 139, Bahía Blanca (8000), Buenos Aires, Argentina. E-mail: fgiannitti@yahoo.com In August 2007 an outbreak of neurological disease and sudden death in Arabian horses occurred in a farm located in Coronel Rosales County, Buenos Aires Province, Argentina. The animals were on a pasture of native grasses and supplemented ad libitum with corn kernels and wheat bran. Three horses were observed having acute neurologic signs including blindness, four leg ataxia, hyperexcitability, aimless walking and circling, followed by death in two of them. Four other horses were found dead overnight without a history of neurologic signs. The morbidity, mortality and lethality rates were 11.6%, 10% and 85.7%, respectively. Grossly, the brain showed focal areas of hemorrhage, brown-yellow discoloration and softening of the sub-cortical white matter. The microscopic brain lesions consisted of extensive areas of malacia within the white matter of the cerebral hemispheres, brainstem and cerebellum, characterized by rarefaction of the white matter with cavitations filled with proteinaceous edema, multifocal hemorrhages and mild infiltration by neutrophils, and rare eosinophils. Swollen glial cells with abundant eosinophilic cytoplasm, distinct cell borders, intracytoplasmic deeply eosinophilic globules and eccentric, hyperchromatic, occasionally pyknotic nucleus were present throughout the areas of rarefaction hemorrhage, edema and necrosis. The feed supplements contained 12,490µg/kg of fumonisin B1 and 5,251µg/kg of fumonisin B2. This is the first reported outbreak of ELEM associated with consumption of feed supplements containing high concentrations of fumonisins in Argentina.

Abstract in Portuguese:

RESUMO.- Giannitti F., Diab S.S., Pacin A.M., Barrandeguy M., Larrere C., Ortega J. & Uzal F.A. 2011. Equine leukoencephalomalacia (ELEM) due to fumonisins B1 and B2 in Argentina. [Leucoencefalomalacia equina devida a fumonisinas B1 e B2 na Argentina.] Pesquisa Veterinária Brasileira 31(5):407-412. Laboratorio de Diagnóstico Veterinario, calle 25 de Mayo 139, Bahía Blanca (8000), Buenos Aires, Argentina. E-mail: fgiannitti@yahoo.com Em agosto de 2007, ocorreu um surto de doença neurológica e morte súbita em cavalos árabes em uma propriedade localizada no município de Coronel Rosales, na província de Buenos Aires, Argentina. Os animais estavam em pasto nativo e eram suplementados ad libitum com grãos de milho e farelo de trigo. Três cavalos foram observados com sinais neurológicos agudos, incluindo cegueira, ataxia nas quatro pernas, hiperexcitabilidade, e andar sem rumo e em círculo, seguidos de morte em dois animais. Outros quatro cavalos foram encontrados mortos durante a noite sem histórico de distúrbios neurológicos. A mortalidade, morbidade e letalidade foram de 11,6%, 10% e 85,7%, respectivamente. Macroscopicamente, o cérebro tinha áreas focais de hemorragia, coloração amarelada e amolecimento da substância branca sub-cortical. Microscopicamente, as lesões cerebrais consistiram de extensas áreas de malácia na substância branca dos hemisférios cerebrais, do tronco encefálico e do cerebelo. Estas lesões da substância branca se caracterizaram por rarefação, cavidades contendo fluido proteináceo (edema), hemorragias multifocais e moderado infiltrado por neutrófilos e raros eosinófilos. Células gliais tumefeitas com abundante citoplasma eosinifílico, limites celulares evidentes, globules citoplasmáticos eosinofílicos, e núcleo excéntrico, hipercromático e ocasionalmente picnótico foram observadas nas areas de rarefacção, edema, hemorragias e necrose. Os suplementos alimentares continham 12.490µg/kg de fumonisina B1 e 5.251µg/kg de fumonisina B2. Este é o primeiro surto reportado na Argentina de leucoencefalomalácia equina associado ao consumo de suplementos alimentares contendo altas concentrações de fumonisinas.


#3 - Immunophrophylactic strategies against enterotoxemia caused by Clostridium perfringens type D in goats

Abstract in English:

Veschi J.L.A., Dutra I.S., Miyakawa M.E.F., Perri S.H.V. & Uzal F.A. 2006. Immunophrophylactic strategies against enterotoxemia caused by Clostridium perfringens type D in goats. Pesquisa Veterinária Brasileira 26(1):51-54. Departamento de Produção e Saúde Animal, Universidade Estadual Paulista, Rua Clóvis Pestana 793, Araçatuba, SP 16050-680, Brazil. E-mail: isdutra@fmva.unesp.br The serological response to an experimental vaccine against Clostridium perfringens type D enterotoxemia was evaluated in four groups of goats. Group 1 received colostrum from unvaccinated cows and no vaccine. Groups 2, 3 and 4 received colostrum from vaccinated cows. In addition, Groups 3 and 4 received a vaccine dose at 80 days of age, and Group 4 received a second vaccine dose at 120 days of age. Serum antibody levels were determined by ELISA in cows before and after calving, and in goats at 3, 80, 120 and 160 days of age. No significant difference in serum antibody levels was observed between vaccinated and unvaccinated cows, or between the four groups of goats evaluated at 3 days of life. Groups 3 and 4 presented mean antibody titers of 0.6 and 1.1 IU/ml, respectively, 40 days after first vaccination. The vaccine response of Group 4 was 1.8 IU/ml 40 days after the booster dose and was higher than that observed for Group 3 (0.2 IU/ml). Thus, in the proposed regimen the use of heterologous colostrum did not induce passive immunization in goat kids. However, first vaccination and a booster dose after 40 days triggered satisfactory antibody levels.

Abstract in Portuguese:

Veschi J.L.A., Dutra I.S., Miyakawa M.E.F., Perri S.H.V. & Uzal F.A. 2006. Immunophrophylactic strategies against enterotoxemia caused by Clostridium perfringens type D in goats. Pesquisa Veterinária Brasileira 26(1):51-54. Departamento de Produção e Saúde Animal, Universidade Estadual Paulista, Rua Clóvis Pestana 793, Araçatuba, SP 16050-680, Brazil. E-mail: isdutra@fmva.unesp.br The serological response to an experimental vaccine against Clostridium perfringens type D enterotoxemia was evaluated in four groups of goats. Group 1 received colostrum from unvaccinated cows and no vaccine. Groups 2, 3 and 4 received colostrum from vaccinated cows. In addition, Groups 3 and 4 received a vaccine dose at 80 days of age, and Group 4 received a second vaccine dose at 120 days of age. Serum antibody levels were determined by ELISA in cows before and after calving, and in goats at 3, 80, 120 and 160 days of age. No significant difference in serum antibody levels was observed between vaccinated and unvaccinated cows, or between the four groups of goats evaluated at 3 days of life. Groups 3 and 4 presented mean antibody titers of 0.6 and 1.1 IU/ml, respectively, 40 days after first vaccination. The vaccine response of Group 4 was 1.8 IU/ml 40 days after the booster dose and was higher than that observed for Group 3 (0.2 IU/ml). Thus, in the proposed regimen the use of heterologous colostrum did not induce passive immunization in goat kids. However, first vaccination and a booster dose after 40 days triggered satisfactory antibody levels.


#4 - Immunohistochemical detection of Clostridia species in paraffin-embedded tissues of experimentally inoculated guinea pigs, p.4-8

Abstract in English:

Assis R.A., Lobato F.C.F., Serakides R., Santos R.L., Dias G.R.C., Nascimento R.A.P., Abreu V.L.V, Parreiras P.M. & Uzal F.A. 2005. Immunohistochemical detection of Clostridia species in paraffin-embedded tissues of experimentally inoculated guinea pigs. Pesquisa Veterinária Brasileira 25(1):4-8. Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Cx. Postal 567, Belo Horizonte, MG 30123-970, Brazil. E-mail: assisra@rwnet.com.br Blackleg is caused by Clostridium chauvoei, whereas malignant oedema is caused by C. chauvoei, C. septicum, C. sordellii, C. perfringens type A, and/or C. novyi type A. Anti-C. chauvoei, anti-C. septicum, anti-C. sordellii and anti-C. novyi type A polyclonal antibodies were produced in rabbits and purified in a column of DEAE-cellulose. Aliquots of the antisera were conjugated with fluorescein isothiocyanate and the remaining was used for the streptavidin biotin peroxidase technique (SBPT). SBPT was standardized to detect C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs. SBPT was compared to a fluorescent antibody technique (FAT). Sections and smears of muscle from inoculation area (MIA), heart, liver, spleen and kidney, were obtained for both SBPT and FAT. Cross-reactions between the different Clostridial species were not observed. C. chauvoei and C. septicum were detected in all specimens from the animals inoculated with these microorganisms, while only sections of muscle obtained from all the animals inoculated with C. sordellii and C. novyi type A were positive. The same results observed by the SBPT, were obtained on tissue smears of these microorganisms stained by the FAT. The results indicate that SBPT is suitable for detection of C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs.

Abstract in Portuguese:

Assis R.A., Lobato F.C.F., Serakides R., Santos R.L., Dias G.R.C., Nascimento R.A.P., Abreu V.L.V, Parreiras P.M. & Uzal F.A. 2005. Immunohistochemical detection of Clostridia species in paraffin-embedded tissues of experimentally inoculated guinea pigs. Pesquisa Veterinária Brasileira 25(1):4-8. Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Cx. Postal 567, Belo Horizonte, MG 30123-970, Brazil. E-mail: assisra@rwnet.com.br Blackleg is caused by Clostridium chauvoei, whereas malignant oedema is caused by C. chauvoei, C. septicum, C. sordellii, C. perfringens type A, and/or C. novyi type A. Anti-C. chauvoei, anti-C. septicum, anti-C. sordellii and anti-C. novyi type A polyclonal antibodies were produced in rabbits and purified in a column of DEAE-cellulose. Aliquots of the antisera were conjugated with fluorescein isothiocyanate and the remaining was used for the streptavidin biotin peroxidase technique (SBPT). SBPT was standardized to detect C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs. SBPT was compared to a fluorescent antibody technique (FAT). Sections and smears of muscle from inoculation area (MIA), heart, liver, spleen and kidney, were obtained for both SBPT and FAT. Cross-reactions between the different Clostridial species were not observed. C. chauvoei and C. septicum were detected in all specimens from the animals inoculated with these microorganisms, while only sections of muscle obtained from all the animals inoculated with C. sordellii and C. novyi type A were positive. The same results observed by the SBPT, were obtained on tissue smears of these microorganisms stained by the FAT. The results indicate that SBPT is suitable for detection of C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs.


#5 - Caprine enterotoxaemia in Rio Grande do Sul, Brazil, 23(4): 173-178

Abstract in English:

ABSTRACT.- Colodel E.M., Driemeier D., Schmitz M., Germer M., Nascimento R.A.P., Assis R.A., Lobato F.C.F. & Uzal F.A. 2003. [Caprine enterotoxaemia in Rio Grande do Sul, Brazil.] Enterotoxemia em caprinos no Rio Grande do Sul. Pesquisa Veterinária Brasileira 23(4):173-178. Setor de Patologia Veterinária, Depto Patologia Clínica Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Cx. Postal 15094, Porto Alegre, RS 91540-000, Brazil. E-mail: moleta@terra.com.br Five outbreaks of caprine enterotoxaemia in Rio Grande do Sul, Brazil, were studied. The animais were found dead or had a clinical course that usually lásted 2-3 hours. From two farms a clinical manifestation period of 12 hours was reported. Clinical signs were characterized by depression, marked abdominal discomfort, profuse watery diarrhea with fibrin clots, and death. Thirteen necropsies were performed and hydropericardium, hydrothorax and hydroperitoneum were commonly found. The mucosa and serosa of the colon were congested, and its contents was watery with multiple fibrin clots. Serosal hemorrhages and fibrin clots in the gallbladder were also seen. Fibrinous colitis and thyphilitis were the most frequent histological changes. Cerebral microangiopathy was observed in one case, which was characterized by acute perivascular proteinaceous edema around arterioles of the nucleus caudatus and thalamus. Large numbers of Gram-positive rods, of ten in clumps and with morphology consistent with Clostridium perfringens, were seen in intestinal smears. The intestinal contents of six goats were positive for epsilon toxin by mouse neutralization test. These findings suggest that enterotoxaemia is an important disease of goat flocks in southern Brazil.

Abstract in Portuguese:

SINOPSE.- Colodel E.M., Driemeier D., Schmitz M., Germer M., Nascimento R.A.P., Assis R.A., Lobato F.C.F. & Uzal F.A. 2003. [Caprine enterotoxaemia in Rio Grande do Sul, Brazil.] Enterotoxemia em caprinos no Rio Grande do Sul. Pesquisa Veterinária Brasileira 23(4):173-178. Setor de Patologia Veterinária, Depto Patologia Clínica Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Cx. Postal 15094, Porto Alegre, RS 91540-000, Brazil. E-mail: moleta@terra.com.br São descritos surtos de enterotoxemia em caprinosem cinco propriedades no Estado do Rio Grande do Sul. Os animais afetados eram, normalmente, encontrados mortos ou apresentavam evolução aguda de 2 a 3 horas com acentuada depressão, cólicas abdominais e diarréia profusa com fibrina. Em duas propriedades relataram-se casos com a evolução de até 12 horas. Em treze animais necropsiados observaram-se aumento de líquidos nas cavidades abdominal, torácica e pericárdica, congestão e hiperemia da serosa e mucosa do intestino, conteúdo do cólon líquido com fibrina além de hemorragias de serosa e fibrina. Em um animal constatou- se microangiopatia cerebral caracterizada por acúmulo de material homogêneo e eosinofilico no espaço perivascular. No conteúdo intestinal, colônias com bastonetes morfológica e bioquímicamente sugestivos de Clostridium pe,fringens foram caracterizadas no estudo bacteriológico. A soroneutralização em camundongos com conteúdo intestinal dos animais afetados, revelou a presença da toxina épsilon. Estes achados evidenciam a enterotoxemia como doença de importância para criação de caprinos no Rio Grande do Sul.


Colégio Brasileiro de Patologia Animal SciELO Brasil CAPES CNPQ UNB UFRRJ CFMV