Resultado da pesquisa (3)

Termo utilizado na pesquisa spores

#1 - Esporos e toxinas de Clostridium botulinum em cacimbas utilizadas como bebedouro de bovinos no Vale do Araguaia, Goiás

Abstract in English:

ABSTRACT.- Souza A.M., Marques D.F., Döbereiner J. & Dutra I.S. 2006. [Spores and toxins of Clostridium botulinum in raining ponds used as water supply for cattle in pastures in the Araguaia Valley, state of Goiás, Brazil.] Esporos e toxinas de Clostridium botulinum em cacimbas utilizadas como bebedouro de bovinos no Vale do Araguaia, Goiás. Pesquisa Veterinária Brasileira 26(3):133-138. Departamento de Medicina Veterinária, Universidade Federal de Goiás, Goiânia, GO 74001-970, Brazil. E-mail: airesms@vet.ufg.br The occurrence and distribution of Clostridium botulinum spores and toxins type C and D in 300 ponds, used by cattle for drinking on 130 farms located in 12 municipalities of the Rio Araguaia valley, state of Goiás, Brazil, was evaluated. The presence of spores was determined indirectly by cultivation in culture medium, followed by inoculation and neutralization in mice of samples of the sediment from the bottom of the raining ponds, from superficial soil and from cattle feces collected to its circuit. The toxin presence was evaluated directly by inoculation in mice of the filtered sediment of the ponds, followed by the neutralization in mice with antitoxins C and D. The presence of C. botulinum spores was significantly more frequent (p<0,05) in the cattle feces (31%), when compared with the results of the superficial soil samples (19%) and the sediments (10%). Botulinum toxins of type C and D or classified as belonging to the CD compound were detected in 6 samples (2%) of the 300 ponds. Of the 130 worked farms, in 122 (93,85%) ponds Clostridium botulinum spores or toxins were found in at least one of the researched variables, whilst ponds on only 8 (6,15%) farms did not present any contamination. Age and depth of the ponds were associated with the frequency of detection of botulinum spores and toxins. The older and shallower the ponds were, the larger was the frequency of isolation of the spores and toxins. The contamination of the ponds in the Araguaia valley with Clostridium botulinum spores and toxins type C and D demonstrates the permanent and growing potential risk for the occurrence of botulism in cattle through drinking water.

Abstract in Portuguese:

ABSTRACT.- Souza A.M., Marques D.F., Döbereiner J. & Dutra I.S. 2006. [Spores and toxins of Clostridium botulinum in raining ponds used as water supply for cattle in pastures in the Araguaia Valley, state of Goiás, Brazil.] Esporos e toxinas de Clostridium botulinum em cacimbas utilizadas como bebedouro de bovinos no Vale do Araguaia, Goiás. Pesquisa Veterinária Brasileira 26(3):133-138. Departamento de Medicina Veterinária, Universidade Federal de Goiás, Goiânia, GO 74001-970, Brazil. E-mail: airesms@vet.ufg.br The occurrence and distribution of Clostridium botulinum spores and toxins type C and D in 300 ponds, used by cattle for drinking on 130 farms located in 12 municipalities of the Rio Araguaia valley, state of Goiás, Brazil, was evaluated. The presence of spores was determined indirectly by cultivation in culture medium, followed by inoculation and neutralization in mice of samples of the sediment from the bottom of the raining ponds, from superficial soil and from cattle feces collected to its circuit. The toxin presence was evaluated directly by inoculation in mice of the filtered sediment of the ponds, followed by the neutralization in mice with antitoxins C and D. The presence of C. botulinum spores was significantly more frequent (p<0,05) in the cattle feces (31%), when compared with the results of the superficial soil samples (19%) and the sediments (10%). Botulinum toxins of type C and D or classified as belonging to the CD compound were detected in 6 samples (2%) of the 300 ponds. Of the 130 worked farms, in 122 (93,85%) ponds Clostridium botulinum spores or toxins were found in at least one of the researched variables, whilst ponds on only 8 (6,15%) farms did not present any contamination. Age and depth of the ponds were associated with the frequency of detection of botulinum spores and toxins. The older and shallower the ponds were, the larger was the frequency of isolation of the spores and toxins. The contamination of the ponds in the Araguaia valley with Clostridium botulinum spores and toxins type C and D demonstrates the permanent and growing potential risk for the occurrence of botulism in cattle through drinking water.


#2 - Occurrence and distribution of Clostridium botulinum type C and D spores in buffalo breeding areas of the Baixada Maranhense, Maranhão, Brazil, 18(3/4):127-131

Abstract in English:

ABSTRACT.- Silva T.M.D., Dutra I.S., Castro R.N. & Döbereiner J. 1998. [Occurrence and distribution of Clostridium botulinum type C and D spores in buffalo breeding areas of the Baixada Maranhense, Maranhão, Brazil.] Ocorrência e distribuição de esporos de Clostridium botulinum tipos C e D em áreas de criação de búfalos na Baixada Maranhense. Pesquisa Veterinária Brasileira 18(3/4):127-131. Depto Apoio, Produção e Saúde Animal, Unesp - Campus de Araçatuba, Cx. Postal 533, Araçatuba, SP 16015-050, Brazil. As botulism is a common disease in butfaloes raised in the low lands of the State of Maranhão, Brazil, the occurrence of Clostridium botulinum spores was evaluated in butfalo breeding areas of 4 municipalities in the &quot;Baixada Maranhense&quot;. Twenty eight samples of faeces, mud and soil were collected and divided into 140 subsamples, being 40 of faeces, 65 of mud and 35 of soil. Botulinum toxin was detected in the filtrates of 104 cultures (74.28%) from 140 subsantples through the inoculation of mice. Using the microcomplement fixation technique for the identification of C. botulinum toxins, type C (14.29%), D (82.14%) and CD complex (3.57%) were found. No significant ditferences (P>0.05%) between faeces, mud and soil samples were observed. There was a high contamination with C. botulinum spores of the butfalo faeces, mud and soil in the areas studied. Identification of other types and subtypes of C. botulinum was not attempted.

Abstract in Portuguese:

RESUMO.- Silva T.M.D., Dutra I.S., Castro R.N. & Döbereiner J. 1998. [Occurrence and distribution of Clostridium botulinum type C and D spores in buffalo breeding areas of the Baixada Maranhense, Maranhão, Brazil.] Ocorrência e distribuição de esporos de Clostridium botulinum tipos C e D em áreas de criação de búfalos na Baixada Maranhense. Pesquisa Veterinária Brasileira 18(3/4):127-131. Depto Apoio, Produção e Saúde Animal, Unesp - Campus de Araçatuba, Cx. Postal 533, Araçatuba, SP 16015-050, Brazil. Botulismo é enzoótico na criação de búfalos da Baixada Maranhense, Estado do Maranhão. No presente trabalho foram realizados estudos para verificar a ocorrência e distribuição de esporos de Clostridium botulinum tipos C e D em amostras de solo, limo e fezes de búfalos, colhidas aleatoriamente em áreas inundáveis da criação de búfalos nessa Baixada. A evidenciação de esporos foi realizada em 40 amostras de fezes, 65 de limo e 35 de solo, provenientes de quatro municípios, pelo cultivo em meio de cultura com carne cozida e posterior inoculação do sobrenadante filtrado em camundongo, na tentativa de verificação da presença de toxina botulínica. A tipificação de amostras positivas foi realizada pela microfixação de complemento. Os resultados revelaram que 104 (74,28%) das 140 amostras examinadas foram positivas para a presença de esporos de C. botulinum pelo teste indireto. Não houve diferença significativa (P>0,05) entre os valores obtidos quando das análises das amostras de solo (77,1%), limo (60,0%) e fezes (95,0%). Das 28 amostras de solo, limo é fezes positivas, que foram utilizadas para a tipificação, quatro (14,29%) foram classificadas como tipo C, 23 (82, 14%) como tipo D e uma (3,5%) como pertencente ao complexo CD. Os resultados revelaram uma alta contaminação ambiental por C. botulinum em áreas de criação de búfalos da Baixada Maranhense. A identificação de outros tipos e de subtipos de C. botulinum não foi realizada.


#3 - Clostridium botulinum spores around decomposed cadavers of bovine victims of botulism in pastores of southern Goias, Brazil

Abstract in English:

Clostridium botulinum spore distribution around thirty decomposed bovine cadavers, presumed to be killed by Botulism, was evaluated in fifteen municipalities in southem Goias (Brazil). Six-hundred-and-thirty soil samples were collected on the site of cadaver decomposition and toward the four cardinal points. Botulin toxin detection from the filtrates of 630 soil cultures was obtained through inoculation of guinea pigs and revealed the presence of 221 cultures (35.07%). The identification of Clostridium botulinum types, using the serum neutralization technique in mice, allowed the recognition of toxins of 204 (32.38%) cultures belonging to five types, being 44 of type A (21.57%); 2 of type B (0.98%); 37 of type C (18.14%); 41 of type D (20.10%); 77 of the CD complex (37.74%) and 3 of type G (1.47%). Types E and F were not found. The toxins of 17 (2.69%) cultures could not be identified, conclusively. The distribution of the C, D and CD complex types around the cadavers was characterized by a negative linear regression influenced directly by the cadavers within a radius of 30 meters.

Abstract in Portuguese:

Foi avaliada a distribuição de esporos de Clostridium botulinum em torno de 30 cadáveres decompostos de bovinos, supostamente vítimas de botulismo, de 15 municípios no sul de Goías. A partir do local em que o cadáver se decompôs e na direção dos quatro pontos cardeais, coletaram-se 630 amostras de solo. A detecção de toxina botulínica dos filtrados das 630 culturas de solo foi obtida pela inoculação em cobaias e revelou a presença em 221 culturas (35,07%). A identificação dos tipos de Clostridium botulinum, utilizando-se a técnica de soro-neutralização em camundongos, permitiu reconhecer as toxinas de 204 (32,38%) culturas pertencentes a cinco tipos, sendo 44 do tipo A (21,57%); dois do tipo B (0,98%); 37 do tipo C (18,14%); 41 do tipo D (20,10%) 77 do complexo CD (37,74%) e três do tipo G (1,47%). Os tipos E e F não foram encontrados. As toxinas de 17 (2,69%) culturas não puderam ser identificadas conclusivamente. A distribuição dos tipos C, D e complexo CD em torno dos cadáveres caracterizou-se por uma regressão linear negativa influenciada diretamente pela presença do cadáver até um raio de 30 metros.


Colégio Brasileiro de Patologia Animal SciELO Brasil CAPES CNPQ UNB UFRRJ CFMV