PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Fecchio R.S., Gomes M.S., Kolososki J., Petri B.S.S., Rossi Jr J.L. & Gioso M.A. 2008. [Study of the occlusion biomechanics and adherence of acrylic resin (polimetilmetacrilate) in fractures of rhinotheca in toucans (Ramphastos toco).] Estudo da biomecânica oclusal e da aderência da resina acrílica auto-polimerizável (polimetilmetacrilato) em fraturas de rinoteca de tucanos (Ramphastos toco). Pesquisa Veterinária Brasileira 28(7):335-340. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Prof. Dr. Orlando de Marques de Paiva 87, Bloco 8 superior, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: rfecchio@gmail.com Traction effort was applied perpendicularly to the axis of toucan beaks and for accomplishment of the essay a dynamometer was used. The beak was completely fractured when submitted to traction of 270.40 N. For fixation of the fractured beaks acrylic resin was applied using a strip of 2.0cm of width. The second beak presented resistance up to 69.75 N. The third beak was submitted to acid conditioning and resisted to force of 63.29 N. Other two new tests were accomplished the whole surface of the rhinotheca filled out. The fourth beak was not submitted to the acid attack and it resisted up to 134.40 N, and the fifth was submitted to the acid etch and it resisted up to 101.50 N. The present work did not observe statistical correlation and, consequently, differences among the procedures with previous use of acid attack and those without the use of the same.

• Toucans rhinotheca fracture beak tucano rinoteca bico Ramphastos toco polimetilmetacrilate polimetilmetacrilato fraturas

Abstract in Portuguese:

ABSTRACT.- Fecchio R.S., Gomes M.S., Kolososki J., Petri B.S.S., Rossi Jr J.L. & Gioso M.A. 2008. [Study of the occlusion biomechanics and adherence of acrylic resin (polimetilmetacrilate) in fractures of rhinotheca in toucans (Ramphastos toco).] Estudo da biomecânica oclusal e da aderência da resina acrílica auto-polimerizável (polimetilmetacrilato) em fraturas de rinoteca de tucanos (Ramphastos toco). Pesquisa Veterinária Brasileira 28(7):335-340. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Prof. Dr. Orlando de Marques de Paiva 87, Bloco 8 superior, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: rfecchio@gmail.com Traction effort was applied perpendicularly to the axis of toucan beaks and for accomplishment of the essay a dynamometer was used. The beak was completely fractured when submitted to traction of 270.40 N. For fixation of the fractured beaks acrylic resin was applied using a strip of 2.0cm of width. The second beak presented resistance up to 69.75 N. The third beak was submitted to acid conditioning and resisted to force of 63.29 N. Other two new tests were accomplished the whole surface of the rhinotheca filled out. The fourth beak was not submitted to the acid attack and it resisted up to 134.40 N, and the fifth was submitted to the acid etch and it resisted up to 101.50 N. The present work did not observe statistical correlation and, consequently, differences among the procedures with previous use of acid attack and those without the use of the same.

Abstract in English:

ABSTRACT.- Lugarini C., Condas L.A.Z., Soresini G.C., Santos R.C.F., Muro M.D., Ono M., Farias M.R. & Montiani-Ferreira F. 2008. Screening of antigenemia and isolation of Cryptococcus neoformans and C. gattii from cloaca and crop of birds in the state of Paraná, Brazil. Pesquisa Veterinária Brasileira 28(7):341-344. Departamento de Medicina Veterinária, Rua dos Funcionários 1540, Juvevê, Curitiba PR 80035-050, Brazil. E-mail: camilelug@gmail.com Cryptococcus neoformans and C. gattii are associated with dry bird excreta but rarely recovered from birds’ digestive tract. The objective of the present study was (1) to verify the existence of C. neoformans and C. gattii in crop and cloaca of wildlife and captivity birds hypothesizing about a possible primary source of this yeast in the excreta, and (2) to determine the fungi’s invasive capability in avian species through latex agglutination. For that purpose, 172 cloacal and 77 crop samples of domestic pigeon, Passerine, and Psittacine birds were collected. None of these samples was positive, suggesting that the yeast is not saprobiotic in the digestive tract of these birds. Only one out of 82 serum samples collected from pigeons and Psittacine birds was positive (title 1:2) showing that Cryptococcus sp. probably has a low invasive capability in birds, and is thus considered only a dry excreta colonizer.

• Ecology birds yeast Cryptococcus neoformans Cryptococcus gattii

Abstract in Portuguese:

ABSTRACT.- Lugarini C., Condas L.A.Z., Soresini G.C., Santos R.C.F., Muro M.D., Ono M., Farias M.R. & Montiani-Ferreira F. 2008. Screening of antigenemia and isolation of Cryptococcus neoformans and C. gattii from cloaca and crop of birds in the state of Paraná, Brazil. Pesquisa Veterinária Brasileira 28(7):341-344. Departamento de Medicina Veterinária, Rua dos Funcionários 1540, Juvevê, Curitiba PR 80035-050, Brazil. E-mail: camilelug@gmail.com Cryptococcus neoformans and C. gattii are associated with dry bird excreta but rarely recovered from birds’ digestive tract. The objective of the present study was (1) to verify the existence of C. neoformans and C. gattii in crop and cloaca of wildlife and captivity birds hypothesizing about a possible primary source of this yeast in the excreta, and (2) to determine the fungi’s invasive capability in avian species through latex agglutination. For that purpose, 172 cloacal and 77 crop samples of domestic pigeon, Passerine, and Psittacine birds were collected. None of these samples was positive, suggesting that the yeast is not saprobiotic in the digestive tract of these birds. Only one out of 82 serum samples collected from pigeons and Psittacine birds was positive (title 1:2) showing that Cryptococcus sp. probably has a low invasive capability in birds, and is thus considered only a dry excreta colonizer.

Abstract in English:

ABSTRACT. Pessoa C.A., Rodrigues M.A., Kozu F.O., Prazeres R.F. & Fecchio R.S. 2008. [Coelioscopic-assisted prefemoral oophorectomy in red-eared slider (Trachemys scripta elegans).] Ooforectomia videoassistida por acesso pré-femural em tartaruga-de-ouvido-vermelho (Trachemys scripta elegans). Pesquisa Veterinária Brasileira 28(7):345-349. Departamento de Epidemiologia Experimental Aplicada às Zoonoses, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Prof. Dr. Orlando de Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: animalexotico@terra.com.br The usual surgical technique for accessing the coelomic cavity of chelonians through the horny shields and bone plates of plastron by using a Dremel machine (plastron osteotomy) is considered slow, traumatic, and very painful for the patient. In this paper a technique of coelioscopic-assisted prefemoral oophorectomy is described in red-eared slider. The time spent on accessing the coelomic cavity, removal of the follicles and ovaries, and anesthetic recovery was markedly decreased (three hours less) in comparison with the traditional technique. The rapid return of the patient to normal activity demonstrates an important reduction in pain at post-surgical recovery. The extra cost resulting of the use of endoscopy and a specialized veterinarian service is clearly justified by the positive results described above.

• Trachemys scripta elegans tartarugas turtles

Abstract in Portuguese:

ABSTRACT. Pessoa C.A., Rodrigues M.A., Kozu F.O., Prazeres R.F. & Fecchio R.S. 2008. [Coelioscopic-assisted prefemoral oophorectomy in red-eared slider (Trachemys scripta elegans).] Ooforectomia videoassistida por acesso pré-femural em tartaruga-de-ouvido-vermelho (Trachemys scripta elegans). Pesquisa Veterinária Brasileira 28(7):345-349. Departamento de Epidemiologia Experimental Aplicada às Zoonoses, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Prof. Dr. Orlando de Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: animalexotico@terra.com.br The usual surgical technique for accessing the coelomic cavity of chelonians through the horny shields and bone plates of plastron by using a Dremel machine (plastron osteotomy) is considered slow, traumatic, and very painful for the patient. In this paper a technique of coelioscopic-assisted prefemoral oophorectomy is described in red-eared slider. The time spent on accessing the coelomic cavity, removal of the follicles and ovaries, and anesthetic recovery was markedly decreased (three hours less) in comparison with the traditional technique. The rapid return of the patient to normal activity demonstrates an important reduction in pain at post-surgical recovery. The extra cost resulting of the use of endoscopy and a specialized veterinarian service is clearly justified by the positive results described above.

Abstract in English:

ABSTRACT.- Graça F.A.S., Peixoto P.V., Coelho C.D., Caldas S.A. & Tokarnia C.H. 2008. [Clinical and pathological aspects of experimental Crotalus poisoning in cattle.] Aspectos clinicos e patológicos do envenenamento crotálico experimental em bovinos. Pesquisa Veterinária Brasileira 28(6):261-270. Projeto Sanidade Animal Embrapa/UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: flaviograca@uol.com.br Crotalus poisoning was experimentally reproduced by subcutaneous inoculation of Crotalus durissus terrificus (South American rattlesnake) venom into 10 clinically healthy mixed bred 12 to 36-month-old cattle, weighing 125 to 449 kg. Two animals were used as controls. The animal that received a dose of 0.03mg/kg body weight died 7h40min after inoculation. A 0.015mg/kg dose provoked death in 4 out of 7 young oxen. Two animals given 0.0075mg/kg became slightly sick and recovered. Onset of symptoms occurred from 1h30min to 13h45min after inoculation. The clinical course varied from 5h25min to 45h for animals that died, and from 33h15min to 17 days for animals that recovered. The main nervous signs observed were diminished response to external stimuli, hypotonic reflexes, dragging of the hooves, apathy, difficulties in moving around obstacles, ocular globe paralysis, lateral and sternal decubitus, and tongue paralysis. Adipsia and sometimes petechiae in the conjunctival and vaginal mucosa were observed. A slight to moderate increase in bleeding time was noted in 6 animals, and a moderate increase in partial thromboplastin time was found in 7 others. Moderate leukocytosis with neutrophilia, relative lymphopenia, eosinopenia, and monocytosis was found. There was a significant increase in creatine kinase serum levels of a ten-fold order. No significant alterations were revealed by urinalysis. Necropsy revealed minimal edema at the inoculation site, few petechiae and equimoses in the epicardium, omentum, biliary vesicle and bladder mucosa of some animals. Histopathological examination revealed necrosis (hyalinization) of groups or isolated myocytes in different muscles examined, both near and far from the inoculation site, in all animals. The diagnosis of Crotalus poisoning and its differentiation from diseases causing paralysis and muscular necrosis in cattle in Brazil are discussed.

• Poisoning Crotalus durissus terrificus cattle snake bite

Abstract in Portuguese:

ABSTRACT.- Graça F.A.S., Peixoto P.V., Coelho C.D., Caldas S.A. & Tokarnia C.H. 2008. [Clinical and pathological aspects of experimental Crotalus poisoning in cattle.] Aspectos clinicos e patológicos do envenenamento crotálico experimental em bovinos. Pesquisa Veterinária Brasileira 28(6):261-270. Projeto Sanidade Animal Embrapa/UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: flaviograca@uol.com.br Crotalus poisoning was experimentally reproduced by subcutaneous inoculation of Crotalus durissus terrificus (South American rattlesnake) venom into 10 clinically healthy mixed bred 12 to 36-month-old cattle, weighing 125 to 449 kg. Two animals were used as controls. The animal that received a dose of 0.03mg/kg body weight died 7h40min after inoculation. A 0.015mg/kg dose provoked death in 4 out of 7 young oxen. Two animals given 0.0075mg/kg became slightly sick and recovered. Onset of symptoms occurred from 1h30min to 13h45min after inoculation. The clinical course varied from 5h25min to 45h for animals that died, and from 33h15min to 17 days for animals that recovered. The main nervous signs observed were diminished response to external stimuli, hypotonic reflexes, dragging of the hooves, apathy, difficulties in moving around obstacles, ocular globe paralysis, lateral and sternal decubitus, and tongue paralysis. Adipsia and sometimes petechiae in the conjunctival and vaginal mucosa were observed. A slight to moderate increase in bleeding time was noted in 6 animals, and a moderate increase in partial thromboplastin time was found in 7 others. Moderate leukocytosis with neutrophilia, relative lymphopenia, eosinopenia, and monocytosis was found. There was a significant increase in creatine kinase serum levels of a ten-fold order. No significant alterations were revealed by urinalysis. Necropsy revealed minimal edema at the inoculation site, few petechiae and equimoses in the epicardium, omentum, biliary vesicle and bladder mucosa of some animals. Histopathological examination revealed necrosis (hyalinization) of groups or isolated myocytes in different muscles examined, both near and far from the inoculation site, in all animals. The diagnosis of Crotalus poisoning and its differentiation from diseases causing paralysis and muscular necrosis in cattle in Brazil are discussed.

Abstract in English:

ABSTRACT.- Oliveira L.I., Jabour F.F., Nogueira V.A. & Yamasaki E.M. 2008. [Experimental poisoning by the leaves of Jatropha gossypifolia (Euphorbiaceae) in sheep.] Intoxicação experimental com as folhas de Jatropha gossypifolia (Euphorbiaceae) em ovinos. Pesquisa Veterinária Brasileira 28(6):275-278. Projeto Sanidade Animal Embrapa/UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: iglesias.vet@gmail.com Fresh green leaves of Jatropha gossypifolia (Euphorbiaceae) were lethal for sheep in single administrations of 40g/kg. The dose of 5g/kg did not cause poisoning, but intermediate doses caused death in part of the animals. The clinical course of poisoning was 6 to 22 days. The clinic and pathological picture in the experimental sheep was characterized by digestive, lung and heart disturbances, and also by slight microscopic liver and renal regressive alterations. These findings are similar to those observed in experiments with the seeds of Jatropha curcas in goats, sheep and calves, and with the fruits and leaves of Jatropha glauca and Jatropha aceroides in goats, performed by other authors. A comparison indicates that, independently of the plant species, the leaves of Jatropha spp. contain toxic compounds similar to those found in the seeds.

• Poisonous plants plant poisoning Jatropha gossypifolia Euphorbiaceae sheep pathology

Abstract in Portuguese:

ABSTRACT.- Oliveira L.I., Jabour F.F., Nogueira V.A. & Yamasaki E.M. 2008. [Experimental poisoning by the leaves of Jatropha gossypifolia (Euphorbiaceae) in sheep.] Intoxicação experimental com as folhas de Jatropha gossypifolia (Euphorbiaceae) em ovinos. Pesquisa Veterinária Brasileira 28(6):275-278. Projeto Sanidade Animal Embrapa/UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: iglesias.vet@gmail.com Fresh green leaves of Jatropha gossypifolia (Euphorbiaceae) were lethal for sheep in single administrations of 40g/kg. The dose of 5g/kg did not cause poisoning, but intermediate doses caused death in part of the animals. The clinical course of poisoning was 6 to 22 days. The clinic and pathological picture in the experimental sheep was characterized by digestive, lung and heart disturbances, and also by slight microscopic liver and renal regressive alterations. These findings are similar to those observed in experiments with the seeds of Jatropha curcas in goats, sheep and calves, and with the fruits and leaves of Jatropha glauca and Jatropha aceroides in goats, performed by other authors. A comparison indicates that, independently of the plant species, the leaves of Jatropha spp. contain toxic compounds similar to those found in the seeds.

Abstract in English:

ABSTRACT.- Souza N.R., Adorno V.B., Marcondes J.S., Oliveira Filho J.P., Conceição L.G., Amorim R.L. & Borges A.S. 2008. [Clinical and histopathological characteristics of the aural plaque in Mangalarga and Quarter Horses.] Características clínicas e histopatológicas da placa aural em eqüinos Mangalarga e Quarto de Milha. Pesquisa Veterinária Brasileira 28(6):279-284. Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Unesp-Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618000, Brazil. E-mail: zep.filho@hotmail.com Aural plaque is a variant of equine papillomatosis. Clinical examination was performed on 306 Mangalarga and 275 Quarter Horses to compare the occurrence of aural plaques among animals and to characterize clinical and histological findings for the disease. Aural plaques occurred in 57% of Mangalarga and in 35% of Quarter breeds. Clinically the lesions consisted of flat, desquamated and hypochromic plaques formed by coalescence of small papules. The main histopathological findings were epidermal hyperplasia and hypomelanosis with abrupt change between the normal and the affected epithelium.

• Equine papillomatosis aural plaques Mangalarga Quarter Horses

Abstract in Portuguese:

ABSTRACT.- Souza N.R., Adorno V.B., Marcondes J.S., Oliveira Filho J.P., Conceição L.G., Amorim R.L. & Borges A.S. 2008. [Clinical and histopathological characteristics of the aural plaque in Mangalarga and Quarter Horses.] Características clínicas e histopatológicas da placa aural em eqüinos Mangalarga e Quarto de Milha. Pesquisa Veterinária Brasileira 28(6):279-284. Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Unesp-Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618000, Brazil. E-mail: zep.filho@hotmail.com Aural plaque is a variant of equine papillomatosis. Clinical examination was performed on 306 Mangalarga and 275 Quarter Horses to compare the occurrence of aural plaques among animals and to characterize clinical and histological findings for the disease. Aural plaques occurred in 57% of Mangalarga and in 35% of Quarter breeds. Clinically the lesions consisted of flat, desquamated and hypochromic plaques formed by coalescence of small papules. The main histopathological findings were epidermal hyperplasia and hypomelanosis with abrupt change between the normal and the affected epithelium.

Abstract in English:

ABSTRACT.- Ferreira L.C.L., Flores E.F., Driemeier D., Melo O. & Lemos R.A.A. 2008. [Mucosal disease associated with generalized dermatitis in cattle, Mato Grosso do Sul, Brazil.] Doença das mucosas associada à dermatite generalizada em bovinos, Mato Grosso do Sul. Pesquisa Veterinária Brasileira 28(6):285-292. Departamento de Medicina Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Müller 2443, Campo Grande, MS 79070-900, Brazil. E-mail: eqrural@nin.ufms.br This paper reports epidemiological, clinical, pathological and laboratory diagnostic aspects of a form of dermatitis associated with mucosal disease (MD) in cattle. It also focuses on the methods used for identifying persistently infected (PI) animals and on the impact of the disease on fertility and weaning rates in the affected herd. Cases of dermatitis associated with MD were diagnosed in two 12 and 24-month-old Nelore calves belonging to a beef cattle farm that operates the full cycle of production (calving, rearing, finishing) in Mato Grosso do Sul, Midwest Brazil. The clinical signs exhibited by affected cattle 0included slow, progressive weight loss; formation of diffuse skin crusts in multiple body areas; skin dryness; multiple ulcerations on the gums and dorsal surface of the tongue which evolved to longitudinal fissures; formation of keratinized projections; and detachment of hoof horn. In addition, diarrhea affected one animal in the late stage of the disease. Necropsies also revealed longitudinal erosions in the esophagus. Histological examination showed coagulation necrosis foci in esophageal and lingual mucosae, with neutrophil and lymphocyte infiltration. Skin lesions consisted of epidermal coagulation necrosis associated with neutrophil infiltration and hyperkeratosis. In both cases, clinical diagnosis was confirmed by the isolation and identification of cytopathic and noncytopathic biotypes of the bovine viral diarrhea virus (BVDV) and immunohistochemical detection of viral antigens in formalin fixed tissues. Out of 300 cattle that had contact with the affected animals, 38 were found to be seropositive - in high neutralizing titers - to BVDV. Blood samples from 1,025 young animals and 40 bulls from the farm were examined for the presence of BVDV to identify potential PI animals. The virus was isolated from blood of three calves in the initial test and, 12 months later, from two of them which had remained on the farm. Only one of these calves was found to be BVDV-positive by immuno-histochemical testing performed on ear-tissue samples. In the year following the birth of PI calves, the herd underwent decreases in fertility and weaning rates, which later returned to their previous levels. The resulting data demonstrate the presence of BVDV infection in beef herds in Mato Grosso do Sul and provide evidence as to include the disease in the differential diagnosis of causes of generalized dermatitis in cattle.

• Bovine viral diarrhea virus mucosal disease cattle diseases viral diseases dermatitis

Abstract in Portuguese:

ABSTRACT.- Ferreira L.C.L., Flores E.F., Driemeier D., Melo O. & Lemos R.A.A. 2008. [Mucosal disease associated with generalized dermatitis in cattle, Mato Grosso do Sul, Brazil.] Doença das mucosas associada à dermatite generalizada em bovinos, Mato Grosso do Sul. Pesquisa Veterinária Brasileira 28(6):285-292. Departamento de Medicina Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Müller 2443, Campo Grande, MS 79070-900, Brazil. E-mail: eqrural@nin.ufms.br This paper reports epidemiological, clinical, pathological and laboratory diagnostic aspects of a form of dermatitis associated with mucosal disease (MD) in cattle. It also focuses on the methods used for identifying persistently infected (PI) animals and on the impact of the disease on fertility and weaning rates in the affected herd. Cases of dermatitis associated with MD were diagnosed in two 12 and 24-month-old Nelore calves belonging to a beef cattle farm that operates the full cycle of production (calving, rearing, finishing) in Mato Grosso do Sul, Midwest Brazil. The clinical signs exhibited by affected cattle 0included slow, progressive weight loss; formation of diffuse skin crusts in multiple body areas; skin dryness; multiple ulcerations on the gums and dorsal surface of the tongue which evolved to longitudinal fissures; formation of keratinized projections; and detachment of hoof horn. In addition, diarrhea affected one animal in the late stage of the disease. Necropsies also revealed longitudinal erosions in the esophagus. Histological examination showed coagulation necrosis foci in esophageal and lingual mucosae, with neutrophil and lymphocyte infiltration. Skin lesions consisted of epidermal coagulation necrosis associated with neutrophil infiltration and hyperkeratosis. In both cases, clinical diagnosis was confirmed by the isolation and identification of cytopathic and noncytopathic biotypes of the bovine viral diarrhea virus (BVDV) and immunohistochemical detection of viral antigens in formalin fixed tissues. Out of 300 cattle that had contact with the affected animals, 38 were found to be seropositive - in high neutralizing titers - to BVDV. Blood samples from 1,025 young animals and 40 bulls from the farm were examined for the presence of BVDV to identify potential PI animals. The virus was isolated from blood of three calves in the initial test and, 12 months later, from two of them which had remained on the farm. Only one of these calves was found to be BVDV-positive by immuno-histochemical testing performed on ear-tissue samples. In the year following the birth of PI calves, the herd underwent decreases in fertility and weaning rates, which later returned to their previous levels. The resulting data demonstrate the presence of BVDV infection in beef herds in Mato Grosso do Sul and provide evidence as to include the disease in the differential diagnosis of causes of generalized dermatitis in cattle.

Abstract in English:

ABSTRACT.- Botteon P.T.L., Ferraz V.P., Glória M.B.A., Melo M.M., Paes Leme F.O., Faleiros R.R. & Alves G.E.S. 2008. [Plasmatic concentrations of tryptamine, tyramine end phenylethylamine in horses under the effect of carbohydrate overload and non-steroid antinflammatory compounds.] Concentrações plasmáticas de triptamina, tiramina e feniletilamina em eqüinos sob efeitos de sobrecarga de carboidratos e antiinflamatórios não esteroidais. Pesquisa Veterinária Brasileira 28(6):299-302. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, 30123-970, Brazil. E-mail: paulobotteon@gmail.com The concentrations of the bioactives amines tryptamine (TRP), tyramine (TYR) and phenylethylamine (PEA) were determined by gas chromatography in plasma samples of 20 horses submitted to carbohydrate overload. Thirty hours after the overload, the horses were randomly distributed in four groups (n=5) and were submitted to four IV treatments every 12 hours: 10ml of saline (GC), ketoprofen 2.2mg/kg (GK), phenylbutazone 4.4mg/kg (GF), and flunixin meglumine 1.1mg/kg (GFM). Blood samples were collected at various times after the overload (0-72 h). Plasma TYR and PEA concentrations ranged from 0.18 to 164.2mg/L, and differed significantly with time (p<0.01), but did not differ in the treatments. Plasma concentrations of TRP differed between times and treatments. The GC was significantly major than other treatments at 48h and 60h after the overload, and the plasma concentration of TRP in groups GK and GFM was significantly lower than in groups GF and GC at 72 h (p=0.0012). We concluded that the anti-inflammatory drugs evaluated do not interfere in the plasma concentration of TYP and PEA. For TRP, ketoprofen and flunixin meglumine was effective to reduce de plasmatic concentration of this amine.

• Amines antinflammatory compounds laminitis horse

Abstract in Portuguese:

ABSTRACT.- Botteon P.T.L., Ferraz V.P., Glória M.B.A., Melo M.M., Paes Leme F.O., Faleiros R.R. & Alves G.E.S. 2008. [Plasmatic concentrations of tryptamine, tyramine end phenylethylamine in horses under the effect of carbohydrate overload and non-steroid antinflammatory compounds.] Concentrações plasmáticas de triptamina, tiramina e feniletilamina em eqüinos sob efeitos de sobrecarga de carboidratos e antiinflamatórios não esteroidais. Pesquisa Veterinária Brasileira 28(6):299-302. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, 30123-970, Brazil. E-mail: paulobotteon@gmail.com The concentrations of the bioactives amines tryptamine (TRP), tyramine (TYR) and phenylethylamine (PEA) were determined by gas chromatography in plasma samples of 20 horses submitted to carbohydrate overload. Thirty hours after the overload, the horses were randomly distributed in four groups (n=5) and were submitted to four IV treatments every 12 hours: 10ml of saline (GC), ketoprofen 2.2mg/kg (GK), phenylbutazone 4.4mg/kg (GF), and flunixin meglumine 1.1mg/kg (GFM). Blood samples were collected at various times after the overload (0-72 h). Plasma TYR and PEA concentrations ranged from 0.18 to 164.2mg/L, and differed significantly with time (p<0.01), but did not differ in the treatments. Plasma concentrations of TRP differed between times and treatments. The GC was significantly major than other treatments at 48h and 60h after the overload, and the plasma concentration of TRP in groups GK and GFM was significantly lower than in groups GF and GC at 72 h (p=0.0012). We concluded that the anti-inflammatory drugs evaluated do not interfere in the plasma concentration of TYP and PEA. For TRP, ketoprofen and flunixin meglumine was effective to reduce de plasmatic concentration of this amine.

Abstract in English:

ABSTRACT.- Caldas S.A., Tokarnia C.H., França T.N., Brito M.F., Graça F.A.S., Coelho C.D. & Peixoto P.V. 2008. [Clinic and pathological and laboratory aspects of experimental poisoning by Bothrops alternatus venom in cattle.] Aspectos clínico-patológicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos. Pesquisa Veterinária Brasileira 28(6):303-312. Projeto Sanidade Animal Embrapa/UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: saulo-andrade@bol.com.br The aim of this study was to determine the clinical-pathological alterations and laboratory findings in cattle inoculated with Bothrops alternatus venom, with the intention of providing information for the establishment of diagnosis and differential diagnosis procedures, as well as to elucidate some obscurities observed in the pertinent literature. The lyophilized venom was diluted in 1 ml of physiologic solution. It was administered to 5 bovines by the subcutaneous route at doses of 0.0625, 0.125 and 0.25mg/kg body weight, and to 2 bovines by the intramuscular route at doses of 0.25 e 0.45mg/kg. Six bovines died and the only animal that survived, who had subcutaneously received the venom at a dose of 0.0625mg/kg, recovered. The first clinical signs were observed from 25min to 5h30min after the inoculation. The clinical evolution time varied from 7 hours 18 minutes to 92 hours. Regardless of the dose, the clinical picture was characterized by swelling (hemorrhage/hematoma) at the site of inoculation, increase in bleeding time and capillary refill time, paleness of mucous membranes and apathy. The laboratory exams revealed progressive normocytic normochromic anemia, thrombocytopenia, reduction in fibrinogen and total plasma proteins, decreased hematocrit and hemoglobin, and slight increase in creatine kinase and lactate dehydrogenase. When subjected to necropsy, the animals that received the venom through the subcutaneous route showed large hematomas and hemorrhagic areas in the subcutaneous tissue, extending from the site of inoculation. The animals in which inoculation was performed intramuscularly exhibited, in addition, intramuscular hemorrhage. The left endocardium showed extensive hemorrhagic lesions, and petechiae were found on the serosae of rumen, omasum, abomasum and gall bladder. The colon, rectum and perirenal areas were surrounded by clotted blood in three animals. Besides hemorrhage, the histological examination revealed hemorrhage and coagulative muscle necrosis in the vicinities of the inoculation site in the animals that received the venom by the intramuscular route. These lesions were mild in the muscles close to the site of inoculation in the animals inoculated by the subcutaneous route. The swelling on the site of inoculation and its surroundings was a consequence of blood accumulation and not merely edema. Myoglobinuria and gross or microscopic lesions were not observed in the kidneys. This study indicates that B. alternatus can lead adult bovines to death if they release all their venom during a bite. Conversely, since venomous ophidians can control the amount of poison they inoculate and they probably do not identify bovines as potential preys, it is likely that the number of accidents involving this species is small, which is in agreement with the findings of the majority of the centers for anatomical-pathological diagnosis in Brazil.

• Bothrops alternatus cattle pathology symptoms

Abstract in Portuguese:

ABSTRACT.- Caldas S.A., Tokarnia C.H., França T.N., Brito M.F., Graça F.A.S., Coelho C.D. & Peixoto P.V. 2008. [Clinic and pathological and laboratory aspects of experimental poisoning by Bothrops alternatus venom in cattle.] Aspectos clínico-patológicos e laboratoriais do envenenamento experimental por Bothrops alternatus em bovinos. Pesquisa Veterinária Brasileira 28(6):303-312. Projeto Sanidade Animal Embrapa/UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: saulo-andrade@bol.com.br The aim of this study was to determine the clinical-pathological alterations and laboratory findings in cattle inoculated with Bothrops alternatus venom, with the intention of providing information for the establishment of diagnosis and differential diagnosis procedures, as well as to elucidate some obscurities observed in the pertinent literature. The lyophilized venom was diluted in 1 ml of physiologic solution. It was administered to 5 bovines by the subcutaneous route at doses of 0.0625, 0.125 and 0.25mg/kg body weight, and to 2 bovines by the intramuscular route at doses of 0.25 e 0.45mg/kg. Six bovines died and the only animal that survived, who had subcutaneously received the venom at a dose of 0.0625mg/kg, recovered. The first clinical signs were observed from 25min to 5h30min after the inoculation. The clinical evolution time varied from 7 hours 18 minutes to 92 hours. Regardless of the dose, the clinical picture was characterized by swelling (hemorrhage/hematoma) at the site of inoculation, increase in bleeding time and capillary refill time, paleness of mucous membranes and apathy. The laboratory exams revealed progressive normocytic normochromic anemia, thrombocytopenia, reduction in fibrinogen and total plasma proteins, decreased hematocrit and hemoglobin, and slight increase in creatine kinase and lactate dehydrogenase. When subjected to necropsy, the animals that received the venom through the subcutaneous route showed large hematomas and hemorrhagic areas in the subcutaneous tissue, extending from the site of inoculation. The animals in which inoculation was performed intramuscularly exhibited, in addition, intramuscular hemorrhage. The left endocardium showed extensive hemorrhagic lesions, and petechiae were found on the serosae of rumen, omasum, abomasum and gall bladder. The colon, rectum and perirenal areas were surrounded by clotted blood in three animals. Besides hemorrhage, the histological examination revealed hemorrhage and coagulative muscle necrosis in the vicinities of the inoculation site in the animals that received the venom by the intramuscular route. These lesions were mild in the muscles close to the site of inoculation in the animals inoculated by the subcutaneous route. The swelling on the site of inoculation and its surroundings was a consequence of blood accumulation and not merely edema. Myoglobinuria and gross or microscopic lesions were not observed in the kidneys. This study indicates that B. alternatus can lead adult bovines to death if they release all their venom during a bite. Conversely, since venomous ophidians can control the amount of poison they inoculate and they probably do not identify bovines as potential preys, it is likely that the number of accidents involving this species is small, which is in agreement with the findings of the majority of the centers for anatomical-pathological diagnosis in Brazil.

Abstract in English:

Abstract.- Zlotowski P., Corrêa A.M.R., Barcellos D.E.S., Cruz C.E.F., Asanome W., Barry A.F., Alfieri A.A. & Driemeier D. 2008. Intestinal lesions in pigs affected with postweaning multisystemic wasting syndrome. Pesquisa Veterinária Brasileira 28(6):313-318. Departamento de Patologia Clinica Veterinária, Faculdade de Medicina Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br Samples of mesenteric lymph nodes and intestines from 79 unthrifty 3- to 5-month-old postweaning pigs, confirmed as naturally affected with postweaning multisystemic wasting syndrome (PMWS), were studied. Pigs originated from 12 farms in southern Brazil and were selected on the basis of clinical signs and/or gross lesions suggestive of enteric disorder. Lymphohistiocytic infiltrates of varying intensity were associated with anti-porcine circovirus type 2 (anti-PCV2) immunostaining (IS) in samples of intestines and mesenteric lymph nodes from all pigs. Although most findings were similar to those described in PCV2-associated enteritis, anti-PCV2 IS in association with depletion of the goblet cell mucin stores (24 pigs), diffuse ileal villous atrophy and fusion (18 pigs), and dilatation of the lymphatic vessels (11 pigs) combined or not with lymphangitis were also observed. PCV2 antigen was immunohistochemically demonstrated in the cytoplasm and nuclei from intralesional epithelial cells, histiocytes, and endothelial-like cells in intestinal tissues. Together these findings imply an association with PCV2. The presence of co-infections by Lawsonia intracellularis, Brachyspira spp., Mycobacterium spp., Salmonella spp., rotavirus, parvovirus, coronavirus and enteric calicivirus with PCV2 in the intestinal lesions was investigated.

• Circovirus co-infections enteric lesions PCV2 PMWS

Abstract in Portuguese:

Abstract.- Zlotowski P., Corrêa A.M.R., Barcellos D.E.S., Cruz C.E.F., Asanome W., Barry A.F., Alfieri A.A. & Driemeier D. 2008. Intestinal lesions in pigs affected with postweaning multisystemic wasting syndrome. Pesquisa Veterinária Brasileira 28(6):313-318. Departamento de Patologia Clinica Veterinária, Faculdade de Medicina Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br Samples of mesenteric lymph nodes and intestines from 79 unthrifty 3- to 5-month-old postweaning pigs, confirmed as naturally affected with postweaning multisystemic wasting syndrome (PMWS), were studied. Pigs originated from 12 farms in southern Brazil and were selected on the basis of clinical signs and/or gross lesions suggestive of enteric disorder. Lymphohistiocytic infiltrates of varying intensity were associated with anti-porcine circovirus type 2 (anti-PCV2) immunostaining (IS) in samples of intestines and mesenteric lymph nodes from all pigs. Although most findings were similar to those described in PCV2-associated enteritis, anti-PCV2 IS in association with depletion of the goblet cell mucin stores (24 pigs), diffuse ileal villous atrophy and fusion (18 pigs), and dilatation of the lymphatic vessels (11 pigs) combined or not with lymphangitis were also observed. PCV2 antigen was immunohistochemically demonstrated in the cytoplasm and nuclei from intralesional epithelial cells, histiocytes, and endothelial-like cells in intestinal tissues. Together these findings imply an association with PCV2. The presence of co-infections by Lawsonia intracellularis, Brachyspira spp., Mycobacterium spp., Salmonella spp., rotavirus, parvovirus, coronavirus and enteric calicivirus with PCV2 in the intestinal lesions was investigated.