PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Cunha V.E. S., Hahnstadt R.L., Soares A.M.B. & Faccini J.L.H. 2007. Evaluation of skin sensitivity in dogs bearing allergic dermatitis to standardized allergenic extracts of house dust mites. Pesquisa Veterinária Brasileira 27(8):341-344. Curso de Pós-Graduação em Ciência Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil E-mail: mvvictor@ufrrj.br The objective of the study was to evaluate whether allergenic extracts of five house dust and storage mite species standardized for humans might be used for the diagnosis of canine atopic dermatitis (CAD). Extracts of Dermatophagoides pteronyssinus (Pyroglyphidae), D. farinae (Pyroglyphidae), Blomia tropicalis (Glycyphagidae), Lepidoglyphus destructor (Glycyphagidae) and Tyrophagus putrescentiae (Acaridae) were evaluated by intradermal testing in 20 healthy dogs (control) and 25 dogs with allergic dermatitis. A significant difference in the response was observed between the two groups (p<0.05). Only one dog (5%) in the control group reacted to the intradermal test, whereas 14 dogs (56%) in the allergic group were positive for at least one extract (odds ratio = 24.2). Most of the positive reactions observed in the allergic group occurred against the extracts of T. putrescentiae or L. destructor, each inducing reactions in 10 dogs (40%). D. farinae, D. pteronyssinus e B. tropicalis extracts induced reactions in 7 (28%), 3 (12%) and 3 (12%) dogs, respectively. The allergenic extracts standardized for humans evaluated in the present study may be used as a tool to complement the diagnosis of the disease, as well as to select potential allergen candidates for allergen-specific immunotherapy.

• Dust mites allergy canine atopic dermatitis intradermal test

Abstract in Portuguese:

ABSTRACT.- Cunha V.E. S., Hahnstadt R.L., Soares A.M.B. & Faccini J.L.H. 2007. Evaluation of skin sensitivity in dogs bearing allergic dermatitis to standardized allergenic extracts of house dust mites. Pesquisa Veterinária Brasileira 27(8):341-344. Curso de Pós-Graduação em Ciência Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil E-mail: mvvictor@ufrrj.br The objective of the study was to evaluate whether allergenic extracts of five house dust and storage mite species standardized for humans might be used for the diagnosis of canine atopic dermatitis (CAD). Extracts of Dermatophagoides pteronyssinus (Pyroglyphidae), D. farinae (Pyroglyphidae), Blomia tropicalis (Glycyphagidae), Lepidoglyphus destructor (Glycyphagidae) and Tyrophagus putrescentiae (Acaridae) were evaluated by intradermal testing in 20 healthy dogs (control) and 25 dogs with allergic dermatitis. A significant difference in the response was observed between the two groups (p<0.05). Only one dog (5%) in the control group reacted to the intradermal test, whereas 14 dogs (56%) in the allergic group were positive for at least one extract (odds ratio = 24.2). Most of the positive reactions observed in the allergic group occurred against the extracts of T. putrescentiae or L. destructor, each inducing reactions in 10 dogs (40%). D. farinae, D. pteronyssinus e B. tropicalis extracts induced reactions in 7 (28%), 3 (12%) and 3 (12%) dogs, respectively. The allergenic extracts standardized for humans evaluated in the present study may be used as a tool to complement the diagnosis of the disease, as well as to select potential allergen candidates for allergen-specific immunotherapy.

Abstract in English:

ABSTRACT.- Binoki D.H., Jatene F.B., Fantoni D.T., Stopiglia A.J., Santos A.L.S., Freitas R.R., Simões E.A., Irino E.T., Monteiro R. & Lofiego R. 2007. [Hemogasometric evaluation in dogs submitted to left pneumonectomy.] Avaliação hemogasométrica em cães submetidos à pneumonectomia esquerda. Pesquisa Veterinária Brasileira 27(8):345-351. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-270, Brazil. E-mail: stopa@usp.br It is well known that different diseases of infectious, traumatic or neoplasic origin can occur in the lungs of dogs, and pneumonectomy technique may be an option for the treatment of some of these diseases. The objective was to evaluate hemogasometric parameters, oxygen partial pressure (PaO2), carbon dioxide partial pressure (PaCO2), hydrogen ion concentration (pH) and bicarbonate ion concentration on the pre- and post-operative moments of the left pneumonectomy. Eighteen adult mongrel dogs, males and females, were randomly distributed into two groups with 9 dogs each. In Group A, the left bronchial stump of the dogs was sutured manually with polypropylene 5-0, and, in Group B, the left bronchial stump of the dogs was sutured mechanically with a surgical stapler. The data were collected at 6 moments: Before the pre-anesthetic administration (T0), one hour after the extubation (T1EXT), 48 hours after the surgery (T48h), 7 days after the surgery (T7d), 15 days after the surgery (T15d), and 36 days after the surgery (T36d). The results were statistically analyzed. PaO2 values of Group A on T1EXT (67.00±11.31) were significantly lower in relation to T0 (99.4±18.34), a fact that did not happen in Group B: T1EXT (87.00±8.35) in regard to T0 (87.00±7.55). There was no difference on pH values in dogs of Group A, but in Group B was observed a decrease on T1EXT (7.3644±0.0353) in relation to T0 (7.4189±0.0136), although the animals did not develop acidosis. It was concluded that dogs submitted to left pneumonectomy (sutured manually or sutured mechanically of left bronchial) did not show immediate and mediate acid-basic instability during the study.

• Pneumonectomy manual suture mechanic suture hemogasometric dogs

Abstract in Portuguese:

ABSTRACT.- Binoki D.H., Jatene F.B., Fantoni D.T., Stopiglia A.J., Santos A.L.S., Freitas R.R., Simões E.A., Irino E.T., Monteiro R. & Lofiego R. 2007. [Hemogasometric evaluation in dogs submitted to left pneumonectomy.] Avaliação hemogasométrica em cães submetidos à pneumonectomia esquerda. Pesquisa Veterinária Brasileira 27(8):345-351. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-270, Brazil. E-mail: stopa@usp.br It is well known that different diseases of infectious, traumatic or neoplasic origin can occur in the lungs of dogs, and pneumonectomy technique may be an option for the treatment of some of these diseases. The objective was to evaluate hemogasometric parameters, oxygen partial pressure (PaO2), carbon dioxide partial pressure (PaCO2), hydrogen ion concentration (pH) and bicarbonate ion concentration on the pre- and post-operative moments of the left pneumonectomy. Eighteen adult mongrel dogs, males and females, were randomly distributed into two groups with 9 dogs each. In Group A, the left bronchial stump of the dogs was sutured manually with polypropylene 5-0, and, in Group B, the left bronchial stump of the dogs was sutured mechanically with a surgical stapler. The data were collected at 6 moments: Before the pre-anesthetic administration (T0), one hour after the extubation (T1EXT), 48 hours after the surgery (T48h), 7 days after the surgery (T7d), 15 days after the surgery (T15d), and 36 days after the surgery (T36d). The results were statistically analyzed. PaO2 values of Group A on T1EXT (67.00±11.31) were significantly lower in relation to T0 (99.4±18.34), a fact that did not happen in Group B: T1EXT (87.00±8.35) in regard to T0 (87.00±7.55). There was no difference on pH values in dogs of Group A, but in Group B was observed a decrease on T1EXT (7.3644±0.0353) in relation to T0 (7.4189±0.0136), although the animals did not develop acidosis. It was concluded that dogs submitted to left pneumonectomy (sutured manually or sutured mechanically of left bronchial) did not show immediate and mediate acid-basic instability during the study.

Abstract in English:

ABSTRACT.- Agreste F.R., Bombonato P.P. & Hernandez-Blazquez, F.J. 2007. [Morphometric characteristics of thymus development in dogs.] Características morfométricas do desenvolvimento do timo em cães. Pesquisa Veterinária Brasileira 27(6):236-240. Departamento de Cirurgia, Setor de Anatomia dos Animais Domésticos e Silvestres, Universidade de São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-000, Brazil. E-mail: bombonat@usp.br The thymus is an organ with great immunological relevance during foetus life and neonatal period, It is the predecessor of lymphopoiesis and shows high lymphopoietic activity, being also the largest lymphoid organ during intrauterine life and at birth. In this study, the morphological aspects of the prenatal development of dog thymus were described in foetus of different ages and sexes. Twenty-four foetus of mongrel domestic dogs, males and females, were divided into four age groups. The thymus presented two lobes linked by a rose-colored tissue connection that was localized in the cranial mediastinal space. The cranial portion protrudes slightly beyond the first pair of ribs. The cells that formed the thymus parenchyma were well organized. Concentric aggregates named Hassal corpuscles were observed, involved by a thin capsule of connective tissue thickened by adipose tissue. The vessels size gradually increased with the foetus age, being larger in animals that were closer to birth. The volume (Vref) and size parameters of female thymus were larger than in males.

• Thymus vascularization lymphatic system morphometry dogs

Abstract in Portuguese:

ABSTRACT.- Agreste F.R., Bombonato P.P. & Hernandez-Blazquez, F.J. 2007. [Morphometric characteristics of thymus development in dogs.] Características morfométricas do desenvolvimento do timo em cães. Pesquisa Veterinária Brasileira 27(6):236-240. Departamento de Cirurgia, Setor de Anatomia dos Animais Domésticos e Silvestres, Universidade de São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-000, Brazil. E-mail: bombonat@usp.br The thymus is an organ with great immunological relevance during foetus life and neonatal period, It is the predecessor of lymphopoiesis and shows high lymphopoietic activity, being also the largest lymphoid organ during intrauterine life and at birth. In this study, the morphological aspects of the prenatal development of dog thymus were described in foetus of different ages and sexes. Twenty-four foetus of mongrel domestic dogs, males and females, were divided into four age groups. The thymus presented two lobes linked by a rose-colored tissue connection that was localized in the cranial mediastinal space. The cranial portion protrudes slightly beyond the first pair of ribs. The cells that formed the thymus parenchyma were well organized. Concentric aggregates named Hassal corpuscles were observed, involved by a thin capsule of connective tissue thickened by adipose tissue. The vessels size gradually increased with the foetus age, being larger in animals that were closer to birth. The volume (Vref) and size parameters of female thymus were larger than in males.

Abstract in English:

ABSTRACT.- Silva M.C., Fighera R.A., Brum J.S., Graça D.L., Kommers G.D., Irigoyen L.F. & Barros C.S.L. 2007. [Clinicopathological features in 620 neurological cases of canine distemper.] Aspectos clinicopatológicos de 620 casos neurológicos de cinomose em cães. Pesquisa Veterinária Brasileira 27(5):215-220. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: claudioslbarros@uol.com.br The files of 5,361 necropsies performed in dogs in the Veterinary Pathology Laboratory of the Federal University of Santa Maria during 1965-2006 were reviewed in search of cases of canine distemper. Six hundred and eighty three cases (12.7%) of the disease were found, 620 of which had neurological signs. From those 620, the following data on each case were retrieved: age, clinical signs, histopathology and concomitance or not of another disease. Age groups were classified as puppies (up to 1 year of age), adults (from 1 to 9 years) and aged (from 10 years on). In 565 out of the 620 (91.1%) neurological cases of canine distemper, histopathological brain changes were observed and in 554 of those 565 the age was registered in the files with following age group distribution: 45.9% of puppies, 51.4% of adults, and 2.7% of aged dogs. Neurological clinical signs encompassed a large spectrum of motor, postural and behavioral disturbances which could occur together or individually. Most frequent clinical signs were myoclonus (38.4%), motor incoordination (25.0%), seizures (18.5%), and paraplegia (13.4%). In 98.4% of the 565 dogs with histopathological changes in the brain demyelination, non-suppurative encephalitis or a combination of these two were found. Intranuclear eosinophilic inclusion bodies were observed in different brain cells of 343 of the 565 dogs with histopathological changes. In 170 (49.6%) the cellular type bearing the inclusions was not mentioned in the file and in the remaining cases the inclusions were seen in astrocytes (94.8% of the cases), neurons (3.5%), oligodendrocytes (1.1%), and ependyma cells (0.6%). Taking in consideration the type of lesions and the age groups, cases with combined demyelination and non-suppurative encephalitis occurred in 40.0% of the puppies, 51.2% of the adult dogs and 72.7% of the aged dogs. Demyelination alone occurred in 48.4% of the puppies, 41.3% of the adults and in 35.7% of the aged dogs. Non-suppurative encephalitis alone occurred 11.6% of the puppies, 7.5% of the adults and in 7.1% of the aged dogs.

• Infectious diseases canine distemper neurology neuropathology pathology diseases of dogs

Abstract in Portuguese:

ABSTRACT.- Silva M.C., Fighera R.A., Brum J.S., Graça D.L., Kommers G.D., Irigoyen L.F. & Barros C.S.L. 2007. [Clinicopathological features in 620 neurological cases of canine distemper.] Aspectos clinicopatológicos de 620 casos neurológicos de cinomose em cães. Pesquisa Veterinária Brasileira 27(5):215-220. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: claudioslbarros@uol.com.br The files of 5,361 necropsies performed in dogs in the Veterinary Pathology Laboratory of the Federal University of Santa Maria during 1965-2006 were reviewed in search of cases of canine distemper. Six hundred and eighty three cases (12.7%) of the disease were found, 620 of which had neurological signs. From those 620, the following data on each case were retrieved: age, clinical signs, histopathology and concomitance or not of another disease. Age groups were classified as puppies (up to 1 year of age), adults (from 1 to 9 years) and aged (from 10 years on). In 565 out of the 620 (91.1%) neurological cases of canine distemper, histopathological brain changes were observed and in 554 of those 565 the age was registered in the files with following age group distribution: 45.9% of puppies, 51.4% of adults, and 2.7% of aged dogs. Neurological clinical signs encompassed a large spectrum of motor, postural and behavioral disturbances which could occur together or individually. Most frequent clinical signs were myoclonus (38.4%), motor incoordination (25.0%), seizures (18.5%), and paraplegia (13.4%). In 98.4% of the 565 dogs with histopathological changes in the brain demyelination, non-suppurative encephalitis or a combination of these two were found. Intranuclear eosinophilic inclusion bodies were observed in different brain cells of 343 of the 565 dogs with histopathological changes. In 170 (49.6%) the cellular type bearing the inclusions was not mentioned in the file and in the remaining cases the inclusions were seen in astrocytes (94.8% of the cases), neurons (3.5%), oligodendrocytes (1.1%), and ependyma cells (0.6%). Taking in consideration the type of lesions and the age groups, cases with combined demyelination and non-suppurative encephalitis occurred in 40.0% of the puppies, 51.2% of the adult dogs and 72.7% of the aged dogs. Demyelination alone occurred in 48.4% of the puppies, 41.3% of the adults and in 35.7% of the aged dogs. Non-suppurative encephalitis alone occurred 11.6% of the puppies, 7.5% of the adults and in 7.1% of the aged dogs.

Abstract in English:

ABSTRACT.- Ferrigno C.R.A., Schmaedecke A. & Ferraz V.C.M. 2007. [A new surgical approach to acetabular denervation in dogs.] Abordagem cirúrgica inédita para denervação acetabular em cães. Pesquisa Veterinária Brasileira 27(1):61-63. Departamento de Cirurgia do Hospital Veterinário, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: cassioaf@usp.br The purpose of this study is to demonstrate a new surgical approach for the selective denervation of the sensitive fibers of the acetabular periosteum, for the treatment of hip dysplasia in dogs, conducted in 189 joints.The surgical approach of the cranial and dorso-lateral regions of the acetabular joint in dogs, by moon-shaped incision of approximately 3cm in extension, starting from the greater trocanter of the femur, in direction of the ileum body. After dorsal opening of the middle gluteal muscle, the insertion of the deep gluteal muscle is inserted for the access to the mentioned regions. With the help of a curette, the periosteum of the cranial and dorso-lateral acetabular margin was removed until the bone cortex was exposed, in all patients. In all animals, the approach was possible, both in the cranial as well as in the caudal faces for the access and curettage of the acetabulum, with the technique described, with a skin incision of about 3.2cm. The curettage of the cranial portion of the acetabulum was, in all cases, realized without the visualization of the bone, but of easy achievement and without intercurrences of nervous or muscular injuries. In all the approaches, it was possible to visualize the articular capsule of the coxo-femoral joint, and in none of the animals, this structure was incised for a technique mistake. The surgical approach described in the present study is feasible for this kind of surgical procedure in dogs, promoting adequate access, with minimal invasion and with no complications of any nature.

• Hip dysplasia surgical technique peripheral nerves dogs surgery

Abstract in Portuguese:

ABSTRACT.- Ferrigno C.R.A., Schmaedecke A. & Ferraz V.C.M. 2007. [A new surgical approach to acetabular denervation in dogs.] Abordagem cirúrgica inédita para denervação acetabular em cães. Pesquisa Veterinária Brasileira 27(1):61-63. Departamento de Cirurgia do Hospital Veterinário, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: cassioaf@usp.br The purpose of this study is to demonstrate a new surgical approach for the selective denervation of the sensitive fibers of the acetabular periosteum, for the treatment of hip dysplasia in dogs, conducted in 189 joints.The surgical approach of the cranial and dorso-lateral regions of the acetabular joint in dogs, by moon-shaped incision of approximately 3cm in extension, starting from the greater trocanter of the femur, in direction of the ileum body. After dorsal opening of the middle gluteal muscle, the insertion of the deep gluteal muscle is inserted for the access to the mentioned regions. With the help of a curette, the periosteum of the cranial and dorso-lateral acetabular margin was removed until the bone cortex was exposed, in all patients. In all animals, the approach was possible, both in the cranial as well as in the caudal faces for the access and curettage of the acetabulum, with the technique described, with a skin incision of about 3.2cm. The curettage of the cranial portion of the acetabulum was, in all cases, realized without the visualization of the bone, but of easy achievement and without intercurrences of nervous or muscular injuries. In all the approaches, it was possible to visualize the articular capsule of the coxo-femoral joint, and in none of the animals, this structure was incised for a technique mistake. The surgical approach described in the present study is feasible for this kind of surgical procedure in dogs, promoting adequate access, with minimal invasion and with no complications of any nature.

Abstract in English:

ABSTRACT.- Ferrigno C.R.A.,Della Nina M.I. & Fantoni D.T. 2007. [A comparative study of osteosynthesis with plates and plates associated with grafts of bone morphogenic proteins (Gen-Techâ) in distal radio-ulna fractures in dogs with less than 6 quilograms.] Estudo comparativo entre as osteossínteses com placas e osteossínteses com placas associadas a enxertos de proteína morfogenética óssea (Gen-Techâ) em fraturas distais de rádio-ulna em cães com menos de 6 quilos. Pesquisa Veterinária Brasileira 27(2):65-69. Departamento de Cirurgia do Hospital Veterinário, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva 87, São Paulo, SP 05508-900. E-mail: cassioaf@usp.br It is well known that bone morphogenic proteins (BMP) cause osteogenesis, yet clinical research must be performed in order to really show their benefits. Animals weighing less than 6 kg show well known difficulties regarding radius and ulna fracture repair mainly with bone non-union, due to poor vascularization of the distal portion of the radius. Therefore this study aimed to compare the velocity of bone callus formation in the treatment of fracture repair with plates and screws alone or with plates and screws plus BMP. Thirty three dogs with radius and ulna fractures were distributed into two groups, where animals of the control group received the conservative treatment performed with screws and plates alone, whilst the other group received the conservative treatment and BMP. The time of bone callus formation was evaluated comparatively through radiographic exams 30, 60, 90, 120, 180 and 210 days after the surgical procedure. Animals treated with BMP showed a healing time of 32±15 days, which was significantly different (p <0.001) from the control group which required 127±34 days. With the results obtained, it can be concluded that the distal radio-ulna fractures of dogs weighing less than 6 kg suffered a significant reduction of the bone callus formation time, which was around 90 days.

• Bone morphogenic proteins BMP bone fractures bone plate dogs

Abstract in Portuguese:

ABSTRACT.- Ferrigno C.R.A.,Della Nina M.I. & Fantoni D.T. 2007. [A comparative study of osteosynthesis with plates and plates associated with grafts of bone morphogenic proteins (Gen-Techâ) in distal radio-ulna fractures in dogs with less than 6 quilograms.] Estudo comparativo entre as osteossínteses com placas e osteossínteses com placas associadas a enxertos de proteína morfogenética óssea (Gen-Techâ) em fraturas distais de rádio-ulna em cães com menos de 6 quilos. Pesquisa Veterinária Brasileira 27(2):65-69. Departamento de Cirurgia do Hospital Veterinário, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva 87, São Paulo, SP 05508-900. E-mail: cassioaf@usp.br It is well known that bone morphogenic proteins (BMP) cause osteogenesis, yet clinical research must be performed in order to really show their benefits. Animals weighing less than 6 kg show well known difficulties regarding radius and ulna fracture repair mainly with bone non-union, due to poor vascularization of the distal portion of the radius. Therefore this study aimed to compare the velocity of bone callus formation in the treatment of fracture repair with plates and screws alone or with plates and screws plus BMP. Thirty three dogs with radius and ulna fractures were distributed into two groups, where animals of the control group received the conservative treatment performed with screws and plates alone, whilst the other group received the conservative treatment and BMP. The time of bone callus formation was evaluated comparatively through radiographic exams 30, 60, 90, 120, 180 and 210 days after the surgical procedure. Animals treated with BMP showed a healing time of 32±15 days, which was significantly different (p <0.001) from the control group which required 127±34 days. With the results obtained, it can be concluded that the distal radio-ulna fractures of dogs weighing less than 6 kg suffered a significant reduction of the bone callus formation time, which was around 90 days.

Abstract in English:

ABSTRACT.- Domingues-F. L.M., Gioso M.A. & Padilha Filho J.G. 2007. Histopathological and histomicrobiological study of root canal therapy medication, comparison of calcium hydroxide versus gutta-percha with zinc oxide/eugenol in the teeth of dogs. Pesquisa Veterinária Brasileira 27(2):71-74. Departamento de Cirurgia da Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando de Marques de Paiva 87, Bloco 8-superior, Cidade Universitária, São Paulo, SP 05508-000, Brazil. E-mail: lesliedf@usp.br The presence of microorganisms in dental structures with experimentally induced necrosis was evaluated. The materials were tested to evaluate their antimicrobial activity and tissue repair efficacy. Four dogs were used in this experiment, with a total of 64 roots of premolar teeth, divided into three groups. The root canals of Group I were filled with gutta-percha and zinc oxide/eugenol cement; Group II were filled with calcium hydroxide, and Group III were not filled. All animals were clinically and radiographically examined 15 days after surgery and then again every subsequent 15 days until 120 days, when the teeth were extracted en bloc. Histopathological analysis showed inflammatory infiltration, cement and bone resorption and necrotic tissue in the apical delta in different proportions. Histomicrobiological analysis showed the presence of microorganisms inside the teeth structures, with different concentrations according to the treatment used. There was statistical significance between the groups (p>0.05). Gutta-percha with zinc oxide/eugenol demonstrated good antimicrobial activity; calcium hydroxide was not efficient. The conclusion of this study is that gutta-percha with zinc oxide/eugenol is the better protocol for filling root canals in dogs.

• Apical delta endodontic gutta-percha calcium hidroxide microorganisms dogs

Abstract in Portuguese:

ABSTRACT.- Domingues-F. L.M., Gioso M.A. & Padilha Filho J.G. 2007. Histopathological and histomicrobiological study of root canal therapy medication, comparison of calcium hydroxide versus gutta-percha with zinc oxide/eugenol in the teeth of dogs. Pesquisa Veterinária Brasileira 27(2):71-74. Departamento de Cirurgia da Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando de Marques de Paiva 87, Bloco 8-superior, Cidade Universitária, São Paulo, SP 05508-000, Brazil. E-mail: lesliedf@usp.br The presence of microorganisms in dental structures with experimentally induced necrosis was evaluated. The materials were tested to evaluate their antimicrobial activity and tissue repair efficacy. Four dogs were used in this experiment, with a total of 64 roots of premolar teeth, divided into three groups. The root canals of Group I were filled with gutta-percha and zinc oxide/eugenol cement; Group II were filled with calcium hydroxide, and Group III were not filled. All animals were clinically and radiographically examined 15 days after surgery and then again every subsequent 15 days until 120 days, when the teeth were extracted en bloc. Histopathological analysis showed inflammatory infiltration, cement and bone resorption and necrotic tissue in the apical delta in different proportions. Histomicrobiological analysis showed the presence of microorganisms inside the teeth structures, with different concentrations according to the treatment used. There was statistical significance between the groups (p>0.05). Gutta-percha with zinc oxide/eugenol demonstrated good antimicrobial activity; calcium hydroxide was not efficient. The conclusion of this study is that gutta-percha with zinc oxide/eugenol is the better protocol for filling root canals in dogs.

Abstract in English:

ABSTRACT.- Domingues-F.L.M., Ferreira J., Lopes F.M., Tymoszczenko A. & Gioso M.A. 2007. The effect of timing temporary cements to treat induced pulp necrosis in the teeth of dogs. Revista Pesquisa Veterinária Brasileira 27(2):85-88. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando de Marques de Paiva 87, Bloco 8-superior, Cidade Universitária, São Paulo, SP 05508-000, Brazil. E-mail: lesliedf@usp.br During endodontic therapy (pulpectomy, root canal debridement and root canal filling) microbiological management is a major concern. Bacteria present in dentine tubules, apical foramina and apical delta are causally related to failure of the procedure. Studies have shown that during single session endodontic treatment bacteria remain within dental structures. The aim of the present study was to evaluate endodontic treatment performed as two sessions, using temporary endodontic dressing materials for different periods in four groups of experimental dogs. A total of 80 roots of second and third upper premolar teeth and second, third and fourth lower premolar teeth were divided into four groups. The pulp chamber was opened with burrs and the pulp exposed for 60 days to induce pulpal inflammation and necrosis. Groups II, III and IV were treated with calcium hydroxide plus camphorated paramono-chlorophenol (PMCC) for 7, 15 and 30 days, respectively. In all groups, the root canals were filled with zinc oxide-eugenol and gutta-percha cones. Clinical and radiographical measurements were performed every 2 weeks. After 60 days a small block section containing the teeth, surrounding periapical tissues and the periodontium was removed for histological and microbiological study. Histological analysis revealed intense inflammatory response in all groups. Microbiological analysis showed microbial reduction inversely proportional to the period of time that the intracanal temporary medicament was left in place.

• Apical delta endodontic gutta-percha camphorated paramonochlorophenol microorganisms dogs

Abstract in Portuguese:

ABSTRACT.- Domingues-F.L.M., Ferreira J., Lopes F.M., Tymoszczenko A. & Gioso M.A. 2007. The effect of timing temporary cements to treat induced pulp necrosis in the teeth of dogs. Revista Pesquisa Veterinária Brasileira 27(2):85-88. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando de Marques de Paiva 87, Bloco 8-superior, Cidade Universitária, São Paulo, SP 05508-000, Brazil. E-mail: lesliedf@usp.br During endodontic therapy (pulpectomy, root canal debridement and root canal filling) microbiological management is a major concern. Bacteria present in dentine tubules, apical foramina and apical delta are causally related to failure of the procedure. Studies have shown that during single session endodontic treatment bacteria remain within dental structures. The aim of the present study was to evaluate endodontic treatment performed as two sessions, using temporary endodontic dressing materials for different periods in four groups of experimental dogs. A total of 80 roots of second and third upper premolar teeth and second, third and fourth lower premolar teeth were divided into four groups. The pulp chamber was opened with burrs and the pulp exposed for 60 days to induce pulpal inflammation and necrosis. Groups II, III and IV were treated with calcium hydroxide plus camphorated paramono-chlorophenol (PMCC) for 7, 15 and 30 days, respectively. In all groups, the root canals were filled with zinc oxide-eugenol and gutta-percha cones. Clinical and radiographical measurements were performed every 2 weeks. After 60 days a small block section containing the teeth, surrounding periapical tissues and the periodontium was removed for histological and microbiological study. Histological analysis revealed intense inflammatory response in all groups. Microbiological analysis showed microbial reduction inversely proportional to the period of time that the intracanal temporary medicament was left in place.

Abstract in English:

Lima V.M.F., Biazzono L., Silva A.C., Correa A.P.F.L. & Luvizotto M.C.R. 2005. Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs. Pesquisa Veterinária Brasileira 25(4):215-218. Departamento de Clínica, Cirurgia e Reprodução Animal, Faculdade de Medicina Veterinária, Universidade Estadual Paulista, Rua Clóvis Pestana 793, Araçatuba, SP 16050-680, Brazil. E-mail: vmflima@fmva.unesp.br A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Leishmania chagasi using total antigen from lysed promastigotes. Fifty symptomatic mixed breed dogs from a region of high incidence of visceral leishmaniasis in Brazil were examined. The results showed that in the positive animals, diagnosed by cytological examination, the ELISA using protein A assay system (mean optical density ± SD / 2.078 ± 0.631) detected more antibodies than the anti-IgG assay (mean optical density ± SD / 1.008 ± 0.437), while in the negative animals, the results by both systems were similar. These results suggest that the ELISA assay using protein A peroxidase conjugated could be useful to detect early infected animals in endemic areas, and thus help to control the spread of the infection.

• Leishmania chagasi visceral leishmaniasis protein A dogs.

Abstract in Portuguese:

Lima V.M.F., Biazzono L., Silva A.C., Correa A.P.F.L. & Luvizotto M.C.R. 2005. Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs. Pesquisa Veterinária Brasileira 25(4):215-218. Departamento de Clínica, Cirurgia e Reprodução Animal, Faculdade de Medicina Veterinária, Universidade Estadual Paulista, Rua Clóvis Pestana 793, Araçatuba, SP 16050-680, Brazil. E-mail: vmflima@fmva.unesp.br A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Leishmania chagasi using total antigen from lysed promastigotes. Fifty symptomatic mixed breed dogs from a region of high incidence of visceral leishmaniasis in Brazil were examined. The results showed that in the positive animals, diagnosed by cytological examination, the ELISA using protein A assay system (mean optical density ± SD / 2.078 ± 0.631) detected more antibodies than the anti-IgG assay (mean optical density ± SD / 1.008 ± 0.437), while in the negative animals, the results by both systems were similar. These results suggest that the ELISA assay using protein A peroxidase conjugated could be useful to detect early infected animals in endemic areas, and thus help to control the spread of the infection.

Abstract in English:

Alves A.L., Madureira R.C., Silva R.A., Corrêa F.N. & Botteon R.C.C.M. 2004. [Frequency of antibodies against Borrelia burgdorferi in dogs from the metropolitan region of Rio de Janeiro.] Freqüência de anticorpos contra Borrelia burgdorferi em cães na região metropolitana do Rio de Janeiro. Pesquisa Veterinária Brasileira 24 (4):203-206. Depto Medicina e Cirurgia Veterinária, Inst. Veterinária, UFRRJ, Seropédica, RJ 23851-970, Brazil. E-mail: fabiolanc@ig.com.br Lyme disease is a worldwide zoonosis caused by Borrelia burgdorferi (Spirochaetae) and transmitted through ticks (Ixodidae). Many species of domestic and wild animals are affected. The dog is an important reservoir in the domiciliary environment. The disease affects the joints and causes alterations in other organs. Blood serum samples from 143 dogs, living in the counties of Seropédica, Itaguaí and Rio de Janeiro, were analyzed with the indirect ELISA test, to search for antibodies against Borrelia burgdorferi latu sensu. Average positive results were 48.25%, 41.9% in Seropédica, 4.9% in Itaguaí and 1.4% in Rio de Janeiro. The titres varied from 1:400 to 1:6400. The prevalence was lower (p< 0.05) in dogs up to one year of age than in older dogs, with significant difference. Between males and females was no statistical difference with positives titres. The result of this survey should draw attention to the occurrence of Borrelia sp in the area studied, considering the importance of Lyme disease as an emerging zoonosis.

• Borrelia burgdorferi Spirochaetae dogs enzyme immunoassay serology.

Abstract in Portuguese:

Alves A.L., Madureira R.C., Silva R.A., Corrêa F.N. & Botteon R.C.C.M. 2004. [Frequency of antibodies against Borrelia burgdorferi in dogs from the metropolitan region of Rio de Janeiro.] Freqüência de anticorpos contra Borrelia burgdorferi em cães na região metropolitana do Rio de Janeiro. Pesquisa Veterinária Brasileira 24 (4):203-206. Depto Medicina e Cirurgia Veterinária, Inst. Veterinária, UFRRJ, Seropédica, RJ 23851-970, Brazil. E-mail: fabiolanc@ig.com.br Lyme disease is a worldwide zoonosis caused by Borrelia burgdorferi (Spirochaetae) and transmitted through ticks (Ixodidae). Many species of domestic and wild animals are affected. The dog is an important reservoir in the domiciliary environment. The disease affects the joints and causes alterations in other organs. Blood serum samples from 143 dogs, living in the counties of Seropédica, Itaguaí and Rio de Janeiro, were analyzed with the indirect ELISA test, to search for antibodies against Borrelia burgdorferi latu sensu. Average positive results were 48.25%, 41.9% in Seropédica, 4.9% in Itaguaí and 1.4% in Rio de Janeiro. The titres varied from 1:400 to 1:6400. The prevalence was lower (p< 0.05) in dogs up to one year of age than in older dogs, with significant difference. Between males and females was no statistical difference with positives titres. The result of this survey should draw attention to the occurrence of Borrelia sp in the area studied, considering the importance of Lyme disease as an emerging zoonosis.