Resultado da pesquisa (12)
Termo utilizado na pesquisa Methyl
#11 - Expression and distribution of connexin 32 in rat liver with experimentally induced fibrosis, p.353-357
Abstract in English:
ABSTRACT.- Santos-Rodrigues A., Dagli M.L.Z., Avanzo J.L., Moraes H.P., Mackowiak I.I. & Hernandez-Blazquez F.J. 2009. Expression and distribution of connexin 32 in rat liver with experimentally induced fibrosis. Pesquisa Veterinária Brasileira 29(4):353-357. Departamento de Cirurgia, Setor de Anatomia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270, Brazil. E-mail: alexsantos@usp.br
The connexin 32 (Cx32) is a protein that forms the channels that promote the gap junction intercellular communication (GJIC) in the liver, allowing the diffusion of small molecules through cytosol from cell-to-cell. Hepatic fibrosis is characterized by a disruption of normal tissue architeture by cellular lesions, and may alter the GJIC. This work aimed to study the expression and distribution of Cx32 in liver fibrosis induced by the oral administration of dimethylnitrosamine in female Wistar rats. The necropsy of the rats was carried out after five weeks of drug administration. They presented a hepatic fibrosis state. Sections from livers with fibrosis and from control livers were submitted to immunohistochemical, Real Time-PCR and Western-Blot analysis to Cx32. In fibrotic livers the Cxs were diffusely scattered in the cytoplasm, contrasting with the control livers, where the Cx32 formed junction plaques at the cell membrane. Also it was found a decrease in the gene expression of Cx32 without reduction in the protein quantity when compared with controls. These results suggest that there the mechanism of intercellular communication between hepatocytes was reduced by the fibrotic process, which may predispose to the occurrence of a neoplastic process, taken in account that connexins are considered tumor suppressing genes.
Abstract in Portuguese:
ABSTRACT.- Santos-Rodrigues A., Dagli M.L.Z., Avanzo J.L., Moraes H.P., Mackowiak I.I. & Hernandez-Blazquez F.J. 2009. Expression and distribution of connexin 32 in rat liver with experimentally induced fibrosis. Pesquisa Veterinária Brasileira 29(4):353-357. Departamento de Cirurgia, Setor de Anatomia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270, Brazil. E-mail: alexsantos@usp.br
The connexin 32 (Cx32) is a protein that forms the channels that promote the gap junction intercellular communication (GJIC) in the liver, allowing the diffusion of small molecules through cytosol from cell-to-cell. Hepatic fibrosis is characterized by a disruption of normal tissue architeture by cellular lesions, and may alter the GJIC. This work aimed to study the expression and distribution of Cx32 in liver fibrosis induced by the oral administration of dimethylnitrosamine in female Wistar rats. The necropsy of the rats was carried out after five weeks of drug administration. They presented a hepatic fibrosis state. Sections from livers with fibrosis and from control livers were submitted to immunohistochemical, Real Time-PCR and Western-Blot analysis to Cx32. In fibrotic livers the Cxs were diffusely scattered in the cytoplasm, contrasting with the control livers, where the Cx32 formed junction plaques at the cell membrane. Also it was found a decrease in the gene expression of Cx32 without reduction in the protein quantity when compared with controls. These results suggest that there the mechanism of intercellular communication between hepatocytes was reduced by the fibrotic process, which may predispose to the occurrence of a neoplastic process, taken in account that connexins are considered tumor suppressing genes.
#12 - Etiology and treatment of bovine mastitis with the help of dimethyl sulfoxide
Abstract in English:
A study was performed in 2 dairy farms on the etiology of bovine mastitis, its control by sanitary measures as well as by treatment with drugs, alone or in combination with dimethyl sulfoxide (DMSO). Staphylococci, streptococci and coliform bacteria were responsible for 79,7% of the cases of mastitis; staphylococci alone caused 55,6%. The measures adopted reduced levels of mastitis from 38 and 21% respectively to 2,7 and 3,1% on the 2 farms. Statistical analysis showed that the combination of drugs with 20% DMSO significantly improved treatments with nitrofurantoin (P < 0.01), chloranphenicol (P < 0.05) and penicilin+streptomycin (P=0,1-0,2). recommending the use of DMSO as vehicle for drugs in the treatment of mastitis.
Abstract in Portuguese:
Em duas granjas leiteiras procedeu-se à Pesquisa sobre a etiologia das mastites bovinas, seu controle por medidas de manejo sanitário e terapêutico com drogas comuns e por sua associação ao dimetilsulfóxido (DMSO), durante 9 trimestres. Os principais agentes foram estafilococos, estreptococos e bactérias coliformes que foram responsáveis por 79 ,7% dos casos, devendo-se aos estafilococos 55 ,6% das mastites. As medidas adotadas reduziram as taxas de mastites de 38 e 21 % respectivamente, para 2,7 e 3,1% em ambas as granjas. A análise estatística mostrou que a associação das drogas ao DMSO a 20% melhorou o índice de curas, com significância (P < 0,01) para o furacin, (P < 0,05) para o cloranfenicol e (P = 0,1-0,2) para a penicilina +estreptomicina, recomendando seu uso como veículo para drogas antimastíticas.
