PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Morini A.C., Brolio M.P., Millano A.M.O.G., Braggio L.Z, Martins D.S., Pere-cin F., Ambrósio C.E. & Miglino M.A. 2011. [There are differences in hematological and biochemical parameters between carriers and not carriers of experimental model of GRMD (Golden Retriever Muscular Dystrophy)?] Existem diferenças nos parâmetros hematológicos e bioquímicos séricos entre fêmeas normais e portadoras do modelo experimental GRMD (Golden Retriever Muscular Dystrophy)? Pesquisa Veterinária Brasileira 31(1):94-98. Setor de Anatomia dos Animais Domésticos e Silvestres, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: mpbrolio@usp.br The purpose of this study was to evaluate whether there are alterations in hematological and biochemical patterns of female Golden Retriever dogs carrying the gene for progressive muscular dystrophy compared to not carriers dogs of the same breed, age and gender. We analyzed 33 animals, divided into two groups; one consisting of 13 not carriers dogs Golden Retrievers (GRNP) and the other composed of 14 dogs Golden Retrievers carrying the gene for muscular dystrophy (GRP). Animals of both groups underwent biochemical and hematological tests with the same frequency and at the same time interval. Although there is a statistically significant difference between groups for some hematological parameters evaluated, all results were in line with the benchmarks used. In the assessment of serum biochemical parameters in the determination of ALT GRS group was slightly above average, but without major clinical significance CK also showed high levels in GRP group, due to muscle degeneration and necrosis characteristic of the disease, the changes found in this analysis were already expected. The other parameters did not change.

• Muscular dystrophy distrofia muscular

Abstract in Portuguese:

RESUMO.- Morini A.C., Brolio M.P., Millano A.M.O.G., Braggio L.Z, Martins D.S., Pere-cin F., Ambrósio C.E. & Miglino M.A. 2011. [There are differences in hematological and biochemical parameters between carriers and not carriers of experimental model of GRMD (Golden Retriever Muscular Dystrophy)?] Existem diferenças nos parâmetros hematológicos e bioquímicos séricos entre fêmeas normais e portadoras do modelo experimental GRMD (Golden Retriever Muscular Dystrophy)? Pesquisa Veterinária Brasileira 31(1):94-98. Setor de Anatomia dos Animais Domésticos e Silvestres, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: mpbrolio@usp.br A proposta deste estudo foi avaliar se existem alterações nos padrões hematológicos e bioquímicos de cadelas da raça Golden Retriever portadoras do gene da distrofia muscular progressiva em comparação aos valores obtidos em cadelas não portadoras de mesma raça e idade. Foram analisados 33 animais, distribuídos em dois grupos, um composto por 19 cadelas Golden Retrievers não portadoras (GRNP) e outro composto por 14 cadelas Golden Retrievers portadoras do gene da distrofia muscular (GRP). Os dois grupos foram submetidos aos mesmos testes hematológicos e bioquímicos, com a mesma frequência e durante o mesmo intervalo de tempo. Apesar de existir diferença estatisticamente significativa entre os grupos para alguns parâmetros hematológicos avaliados, todos os resultados obtidos estavam de acordo com os valores de referência utilizados. Na avaliação dos parâmetros bioquímicos séricos a dosagem de ALT no grupo GRNP ficou levemente acima da média, porém sem grandes significados clínicos A CK também apresentou níveis elevados no grupo GRP, devido à degeneração e necrose muscular característicos da doença, as alterações encontradas nessa análise já eram esperadas. Os demais parâmetros não se alteraram.

Abstract in English:

ABSTRACT.- Gerger A.A.C., Souza C.C., Martins D.S., Gaiad T., Brolio M.P., Luppi M.R., Ambrósio C.E. & Miglino M.A. 2010. [Changes of the digestive tract of Golden Retriever dogs affected by muscular dystrophy.] Alterações do trato digestório de cães da raça Golden Retriever afetados pela distrofia muscular. Pesquisa Veterinária Brasileira 30(12):1064-1070. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508 270, Brazil. E-mail: ccostola@usp.br The experimental canine model Golden Retriever carrier of Muscular Dystrophy (GRMD) is the best substitute of animal models to study Duchenne Muscular Dystrophy. Above striated muscle, the disease can affect the heart and smooth muscle, so the functioning of the digestive tract, as the smooth muscle is the primary element of tubular organs. Through morphological description, the purpose of this study was to determine whether the muscular dystrophy affects the overall architecture of the digestive tract and how is willing this muscular structure. Were evaluated macroscopic and microscopic optical description staining with hematoxylin-eosin, Masson’s trichrome and Sirius. The esophagus and liver of affected animals were altered. The stomach of the animals did not occupy the usual space. The diaphragm muscle had atrophied. The general histological structure of the digestive tract presented in a manner similar to a normal animal. Changes and histological differences were found in the muscle layer.

• Muscular dystrophy distrofia muscular

Abstract in Portuguese:

ABSTRACT.- Gerger A.A.C., Souza C.C., Martins D.S., Gaiad T., Brolio M.P., Luppi M.R., Ambrósio C.E. & Miglino M.A. 2010. [Changes of the digestive tract of Golden Retriever dogs affected by muscular dystrophy.] Alterações do trato digestório de cães da raça Golden Retriever afetados pela distrofia muscular. Pesquisa Veterinária Brasileira 30(12):1064-1070. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508 270, Brazil. E-mail: ccostola@usp.br O modelo experimental canino Golden Retriever portador da Distrofia Muscular (GRMD) é o melhor substituto entre os modelos animais para estudar a Distrofia Muscular de Duchenne. Além da musculatura estriada, a doença pode afetar a musculatura estriada cardíaca e a musculatura lisa, e desta forma, o funcionamento do trato digestório, já que o músculo liso é o elemento primário dos órgãos tubulares. Através de estudo morfológico descritivo, o objetivo deste trabalho foi verificar se a distrofia muscular afeta a arquitetura geral do trato digestório e como se dispõe sua estrutura muscular em animais afetados. Foram realizadas avaliações descritivas macro e microscópicas com colorações de Hematoxilina-Eosina, Tricrômio de Masson e Picrosirius. Entre os resultados apresentados, verificou-se que o esôfago e o fígado dos animais afetados encontraram-se alterados, assim como o estômago não ocupava seu lugar habitual. O músculo diafragma apresentava-se atrofiado e diferenças histológicas foram encontradas na camada muscular do sistema gastrointestinal, em geral. Outras estruturas do tubo digestório de GRMDs apresentaram-se de maneira similar a de um animal normal.

Abstract in English:

ABSTRACT.- Bellatine T., Mançanares C.A.F., Franciolli A.L.R., Ambrósio C.E., Martins D.S., Miglino M.A. & Carvalho A.F. 2010. [Morphofunctional study of Crab-eating Raccoon (Procyon cancrivorus) mammary gland.] Estudo morfofuncional das glândulas mamárias de Mão Pelada, Procyon cancrivorus. Pesquisa Veterinária Brasileira 30(8):689-695. Departamento de Morfologia, Faculdade de Medicina Veterinária, Centro Universitário da Fundação de Ensino Octávio Bastos, Avenida Octávio Bastos s/n, Jardim Nova São João, São João da Boa Vista, SP 13870-000, Brazil. E-mail: labvet01@unifeob.edu.br Three Procyon cancrivorus females were studied with emphasis for gross and microscopical description of the mammary glands. Samples of the glands were processed with routine techniques for histology. The females studied presented three pairs of mammary glands: one pair of cranial abdominal mammary glands, a second pair of caudal abdominal and a third one, as inguinal mammary glands. Mammary papillae presented a pendulum shape, as in the domestic dogs. Microscopically, the mammary gland consisted from the external to the internal portion (1) of stratified squamous epithelium of the epidermis, (2) dense irregular connective tissue of the derma, and (3) smooth muscle fibers and papillary ducts that flowed with “shower” shape into the lactiferous sinus. The secretory portion consisted of tubule-alveolar glands with cuboidal cells disposed in a simple layer. The results indicate that the set of glandular studies is similar to ones related to dog (Canis familiaris) in such a way in its gross aspect that how much in its microscopically structure. This fact suggests that we can use the raccoon and the dog as similar models of study, for identification of pathologies related to this system.

• Procyon cancrivorus raccoon mammary gland Crab-eating Raccoon guaxinim mão-pelada glândula mamária

Abstract in Portuguese:

RESUMO.- Bellatine T., Mançanares C.A.F., Franciolli A.L.R., Ambrósio C.E., Martins D.S., Miglino M.A. & Carvalho A.F. 2010. [Morphofunctional study of Crab-eating Raccoon (Procyon cancrivorus) mammary gland.] Estudo morfofuncional das glândulas mamárias de Mão Pelada, Procyon cancrivorus. Pesquisa Veterinária Brasileira 30(8):689-695. Departamento de Morfologia, Faculdade de Medicina Veterinária, Centro Universitário da Fundação de Ensino Octávio Bastos, Avenida Octávio Bastos s/n, Jardim Nova São João, São João da Boa Vista, SP 13870-000, Brazil. E-mail: labvet01@unifeob.edu.br Para a descrição macro e microscópica das glândulas mamárias foram utilizadas três fêmeas de Mão Pelada (Procyon cancrivorus). As amostras das glândulas foram processadas conforme técnicas rotineiras para histologia. As fêmeas estudadas apresentaram 3 pares de glândulas mamárias, sendo um par de glândula mamária abdominal cranial, um par de abdominal caudal e um par de inguinal. As papilas mamárias apresentaram formato pendular, como os canídeos domésticos. Microscopicamente, a glândula mamária apresentou da porção externa para a interna: epiderme (epitélio estratificado pavimentoso queratinizado), derme (tecido conjuntivo frouxo e tecido conjuntivo denso não modelado), fibras musculares lisas e ductos papilíferos que abrem em vários ósteos papilares em formato de “chuveiro”. A porção secretora glandular era caracteristicamente túbulo alveolar, com células cuboidais dispostas em camada simples. Os resultados indicam que o conjunto glandular estudado é semelhante ao da cadela (Cannis familiaris) tanto em seu aspecto macroscópico quanto em seu aspecto microscópico, este fato sugere que podemos utilizar o Mão Pelada e o Cão como modelos similares de estudo, para identificação de patologias relacionadas a este sistema.

Abstract in English:

RESUMO.- Esteves P.R., Araújo K.P.C., Teixeira D.G., Ambrósio C.E., Martins D.S., Morini A.C., Morini Junior J.C., Duarte C.N., Bombonato P.P., Miglino M.A. & Mariana A.N.B. 2009. Study of the cardiac left atrioventricular valvar complex in water buffaloes (Bubalus bubalis) of the Jafarabadi breed. [Estudo do complexo valvar atrioventricular cardíaco esquerdo em búfalos (Bubalus bubalis) da raça Jafarabadi.] Pesquisa Veterinária Brasileira 29(10):852-858. Setor de Anatomia dos Animais Domésticos e Silvestres, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo. Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 05508- 900, Brazil. E-mail: ceambrosio@usp.br [Estudo do complexo valvar atrioventricular cardíaco esquerdo em búfalos (Bubalus bubalis) da raça Jafarabadi.] Foram estudados os complexos valvares atrioventricular esquerdo de 30 búfalos da raça Jafarabadi, machos e adultos, sem alterações cardíacas, provenientes de abatedouros do Estado do Paraná. Os corações foram examinados em seu terço médio para acesso ao complexo valvar, que foi submetido a estudos de área, número e tipificação de cordas tendíneas. Os resultados demonstram que este complexo é formado por duas cúspides principais e quatro cúspides acessórias, apresentam em sua formação de 2-3 músculos papilares, nos quais se inserem de 10-25 cordas tendíneas, que se fixam em cúspides voltadas para a parede do ventrículo. A área total deste complexo apresenta uma média de 38,56cm2 com um mínimo de 24,96cm2 e um máximo de 55,54cm2. Estatisticamente não há relação entre número de cordas e a área da cúspide onde estas estão inseridas, nem com o número de músculos papilares dos quais elas provem.

• Heart Jaffarabadi water buffaloes cardiac ventricle mitral valve

Abstract in Portuguese:

ABSTRACT.- Esteves P.R., Araújo K.P.C., Teixeira D.G., Ambrósio C.E., Martins D.S., Morini A.C., Morini Junior J.C., Duarte C.N., Bombonato P.P., Miglino M.A. & Mariana A.N.B. 2009. Study of the cardiac left atrioventricular valvar complex in water buffaloes (Bubalus bubalis) of the Jafarabadi breed. Pesquisa Veterinária Brasileira 29(10):852-858. Setor de Anatomia dos Animais Domésticos e Silvestres, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo. Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 05508- 900, Brazil. E-mail: ceambrosio@usp.br Atrioventricular valve complex of 30 Jafarabadi water buffaloes, adult males were studied in this research with no heart diseases. The animals were obtained from a slaughterhouse in Brazilian State of Parana. The hearts were opened at the third portion affording access to the valve complex. The complexes had its area, number and type of tendinous cords submitted to analysis. The results showed that the complex is composed by two cusps and four accessory cusps, two or three papillary muscles in which 10-25 tendinous cords fix on the cusps that face the ventricle wall. The total area of the complex was on average 38.56cm2, with a minimum of 24.96cm2 and a maximum of 55.54cm2. Statistically, no relation between the number of cords and the cusps’ area where they are inserted or with the number of papillary muscle where they originated from was observed.

Abstract in English:

ABSTRACT.- Barbosa P.L.G., Melnic R.V., Morini A.C., Martins D.S., Morini-Junior J.C., Pereira F.T.V., Franciolli A.L.R., Favaron P.O., Ambrósio C.E. & Miglino M.A. 2008. [Characterization of the fetal membranes in buffalo cows in the first three months of gestation.] Caracterização das membranas fetais em búfalas no terço inicial da gestação. Pesquisa Veterinária Brasileira 28(9):437-445. Setor de Anatomia dos Animais Domésticos e Silvestres, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-900, Brazil. E-mail: drimorini@usp.br The aim of the study was to characterize the fetal membranes in buffalo cows (Bubalus bubalis, Linnaeus 1758) in the first 3 months of the gestation. The fetal membranes were analyzed gross and microscopically (light and ultrastructural analysis). The corion is a simple layer of circle cells, with spherical nucleus, named trofoblastics; there is another cellular type, named trophoblastic giant cells, with two or more nucleus. Both of them have many cytoplasmic and reticule endoplasmic vesicles on transmission electron microscopy. The allantoic has many vessels filled with eritrocytes, elongated cells which form a stratified simple epithelium. The amnion is transparent or sometimes whitish, consisting of a stratified simple epithelium. The main difference between the allantoic and amnion is that the latter is avascular. The yolk sac is an opaque membrane that disappears during gestation, and is the only membrane that is not in contact with the others; it presents three different types of cells which form three distinct layers (endoderm, mesothelium and mesenquimal).

• Buffaloes fetal membranes morphology placenta

Abstract in Portuguese:

ABSTRACT.- Barbosa P.L.G., Melnic R.V., Morini A.C., Martins D.S., Morini-Junior J.C., Pereira F.T.V., Franciolli A.L.R., Favaron P.O., Ambrósio C.E. & Miglino M.A. 2008. [Characterization of the fetal membranes in buffalo cows in the first three months of gestation.] Caracterização das membranas fetais em búfalas no terço inicial da gestação. Pesquisa Veterinária Brasileira 28(9):437-445. Setor de Anatomia dos Animais Domésticos e Silvestres, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-900, Brazil. E-mail: drimorini@usp.br The aim of the study was to characterize the fetal membranes in buffalo cows (Bubalus bubalis, Linnaeus 1758) in the first 3 months of the gestation. The fetal membranes were analyzed gross and microscopically (light and ultrastructural analysis). The corion is a simple layer of circle cells, with spherical nucleus, named trofoblastics; there is another cellular type, named trophoblastic giant cells, with two or more nucleus. Both of them have many cytoplasmic and reticule endoplasmic vesicles on transmission electron microscopy. The allantoic has many vessels filled with eritrocytes, elongated cells which form a stratified simple epithelium. The amnion is transparent or sometimes whitish, consisting of a stratified simple epithelium. The main difference between the allantoic and amnion is that the latter is avascular. The yolk sac is an opaque membrane that disappears during gestation, and is the only membrane that is not in contact with the others; it presents three different types of cells which form three distinct layers (endoderm, mesothelium and mesenquimal).

Abstract in English:

ABSTRACT.- Conceição R.A., Ambrósio C.E., Martins D.S., Carvalho A.F., Franciolli A.L.R., Machado M.R.F., Oliveira M.F. & Miglino M.A. 2008. [Morphological aspects of yolk sac from rodents of Hystricomorpha subordem: paca (Agouti paca) and agouti (Dasyprocta aguti).] Aspectos morfológicos do saco vitelino em dois roedores da subordem Hystricomorpha: paca (Agouti paca) e cutia (Dasyprocta aguti). Pesquisa Veterinária Brasileira 28(5):253-259. Setor de Anatomia dos Animais Domésticos e Silvestres, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia. Universidade de São Paulo, Cidade Universitária, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: ceambrosio@usp.br The study aimed to characterize gross and microscopic features of the yolk sac in paca (Agouti paca) and agouti (Dasyprocta aguti) in early gestation. Fragments of the yolk sac of 3 paca and 3 agouti fetuses at early gestation were taken and processed for histological and ultrastructural analyses. Gross features of the vitelline placenta in both species showed its insertion over the main placenta surface and projections to the embryos/fetuses. Microscopically, the vitelline placenta was constituted by endoderm epithelium and mesenchyme, in which vitelline vessels are abundant. The ultrastructure of the samples showed that the visceral yolk sac of the paca was formed by endodermic cells with nuclei in the median region, and that the visceral yolk sac of the agouti was formed by nuclei arranged apically; other characteristic was the large number of mitochondrias, eletrodense vesicles with microvilosities We conclude that (1) the vitelline placenta of the two species presents insertion in the surface of the main placenta; (2) the vitelline placenta of paca rests on the Reichert’s membrane, whereas the agouti vitelline placenta does not have this membrane; (3) the chorion and allantoic are fusioned; and (4) the chorioallantoic placenta and the yolk sac in both species are reversed and vascularized.

• Hystricomorpha Agouti paca Dasyprota aguti yolk sac

Abstract in Portuguese:

ABSTRACT.- Conceição R.A., Ambrósio C.E., Martins D.S., Carvalho A.F., Franciolli A.L.R., Machado M.R.F., Oliveira M.F. & Miglino M.A. 2008. [Morphological aspects of yolk sac from rodents of Hystricomorpha subordem: paca (Agouti paca) and agouti (Dasyprocta aguti).] Aspectos morfológicos do saco vitelino em dois roedores da subordem Hystricomorpha: paca (Agouti paca) e cutia (Dasyprocta aguti). Pesquisa Veterinária Brasileira 28(5):253-259. Setor de Anatomia dos Animais Domésticos e Silvestres, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia. Universidade de São Paulo, Cidade Universitária, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: ceambrosio@usp.br The study aimed to characterize gross and microscopic features of the yolk sac in paca (Agouti paca) and agouti (Dasyprocta aguti) in early gestation. Fragments of the yolk sac of 3 paca and 3 agouti fetuses at early gestation were taken and processed for histological and ultrastructural analyses. Gross features of the vitelline placenta in both species showed its insertion over the main placenta surface and projections to the embryos/fetuses. Microscopically, the vitelline placenta was constituted by endoderm epithelium and mesenchyme, in which vitelline vessels are abundant. The ultrastructure of the samples showed that the visceral yolk sac of the paca was formed by endodermic cells with nuclei in the median region, and that the visceral yolk sac of the agouti was formed by nuclei arranged apically; other characteristic was the large number of mitochondrias, eletrodense vesicles with microvilosities We conclude that (1) the vitelline placenta of the two species presents insertion in the surface of the main placenta; (2) the vitelline placenta of paca rests on the Reichert’s membrane, whereas the agouti vitelline placenta does not have this membrane; (3) the chorion and allantoic are fusioned; and (4) the chorioallantoic placenta and the yolk sac in both species are reversed and vascularized.

Abstract in English:

ABSTRACT.- Santos D.A.S., Ricci R.E.G., Prado I.M.M., Carvalho A.F., Bombonato P.P., Ambrósio C.E., Teixeira D.G., Santos T.C., H.-Brazquez F.J., Martins D.S., Morini A.C., Kfoury Jr J.R. & Miglino M.A. 2007. [Morphology and morphometry of buffalo mammary papillae.] Morfologia e morfometria das papilas mamárias de búfalas. Pesquisa Veterinária Brasileira 27(3):95-102. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: ceambrosio@usp.br Fifteen papillae from female water buffaloes and one bovine female were obtained in a slaughter house for this study, which consisted in morphologic and biometrical analyses, histology and histometric and mesoscopical analyses. The means were: right cranial papilla (RCP) 20.95g; right caudal papilla (RCaP) 21.49g; left cranial papilla (LCP) 23.52g; and left caudal papilla (LCaP) 21.35g. Papillary duct average length, numbers of folds and papillary cisterns length were, respectively: 0.67/5,5 e 2.17cm for RCP; 0.62/6.0 e 2.26cm for RCaP; and for the left antimere: RCP: 0.71/6.0 e 2.26cm and for RCaP: 0.74/5.7 e 2.57cm. Pearson’s analysis revealed negative correlation between body weight and mammary glandular weight. Sampling of proximal, middle and distal area was taken and fixed into a 10% formaldehyde solution, followed by mesoscopical investigation through 10, 20 and 40µm thickness cuts stained by Azan, F3BA Picrosirus and Weigert-Van Gieson techniques. Histological and morphometric analyses were applied on 5µm thick samples and showed that the muscular layer was disposed into two arrangements, internal circular and external longitudinal with a bi-stratified cuboidal epithelium. A well developed muscular layer were related to the middle portion revealing thickness of 22.90mm with a great amount of connective tissue (7.10mm).

• Buffaloes mammary papilla morphometry morphology Buballus bubalis

Abstract in Portuguese:

ABSTRACT.- Santos D.A.S., Ricci R.E.G., Prado I.M.M., Carvalho A.F., Bombonato P.P., Ambrósio C.E., Teixeira D.G., Santos T.C., H.-Brazquez F.J., Martins D.S., Morini A.C., Kfoury Jr J.R. & Miglino M.A. 2007. [Morphology and morphometry of buffalo mammary papillae.] Morfologia e morfometria das papilas mamárias de búfalas. Pesquisa Veterinária Brasileira 27(3):95-102. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: ceambrosio@usp.br Fifteen papillae from female water buffaloes and one bovine female were obtained in a slaughter house for this study, which consisted in morphologic and biometrical analyses, histology and histometric and mesoscopical analyses. The means were: right cranial papilla (RCP) 20.95g; right caudal papilla (RCaP) 21.49g; left cranial papilla (LCP) 23.52g; and left caudal papilla (LCaP) 21.35g. Papillary duct average length, numbers of folds and papillary cisterns length were, respectively: 0.67/5,5 e 2.17cm for RCP; 0.62/6.0 e 2.26cm for RCaP; and for the left antimere: RCP: 0.71/6.0 e 2.26cm and for RCaP: 0.74/5.7 e 2.57cm. Pearson’s analysis revealed negative correlation between body weight and mammary glandular weight. Sampling of proximal, middle and distal area was taken and fixed into a 10% formaldehyde solution, followed by mesoscopical investigation through 10, 20 and 40µm thickness cuts stained by Azan, F3BA Picrosirus and Weigert-Van Gieson techniques. Histological and morphometric analyses were applied on 5µm thick samples and showed that the muscular layer was disposed into two arrangements, internal circular and external longitudinal with a bi-stratified cuboidal epithelium. A well developed muscular layer were related to the middle portion revealing thickness of 22.90mm with a great amount of connective tissue (7.10mm).

Abstract in English:

ABSTRACT.- Farias M.M.M.D, Wenceslau C.V., Passos J., Teixeira D.G., Araújo K.P.C., Ambrósio C.E., Martins D.S. & Prada I.L.S. 2007. [Behavior of the glossopharyngeal and vagus nerves in the retropharyngeal region of sheep: apparent origin in cranium, course, branching and dis-tribution.] Comportamento dos nervos glossofaríngeo e vago, na região retrofaríngea de ovinos: origem aparente no crânio, trajeto, ramificação e distribuição. Pesquisa Veterinária Brasileira 27(3):115-123. Setor de Anatomia dos Animais Domésticos e Silvestres, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia. Universidade de São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-900, Brazil. E-mail: ceambrosio@usp.br In 60 hemiheads of sheep of the Santa Ines breed the apparent origin in the skull of itinerary, ramification and distribution of the glossopharingeal nerve (Gf) and the vagus nerve (Vg) in the retropharyngeal region (Rr) were studied. By fixation and dissection of the specimens it was seen that the glossopharyngeal nerve and the vagus nerve arise from the jugular foramen in 100% of the cases. The right and the left glossopharingeal nerve (Glde) are frequently (86.6%) located more medially to the tympanic bulla, pass caudally to the stylohyoid bone, bypass the margin of the caudal stylopharyngeal muscle, the tonsilla, of the pharyngeal and the lingual mucous membrane. These branches are distributed, respectively, in the carotid sinus, pharyngeal musculature, soft palate, stylopharyngeal muscle, palatine tonsil, pharyngeal mucosa and the caudal third of the tongue (100%). The right and the left vagus nerve run caudally in association with the accessory nerves (Ac) up to the atlas (70%) and go then medially to the occipital artery, dorsally to the common carotid and the sympathetic trunk in the cervical region (80%). The vague nerves have pharyngeal (86.6%) and cranial laryngeal (100%) branches.

• Anatomy sheep glossopharingeal nerve vagus nerve

Abstract in Portuguese:

ABSTRACT.- Farias M.M.M.D, Wenceslau C.V., Passos J., Teixeira D.G., Araújo K.P.C., Ambrósio C.E., Martins D.S. & Prada I.L.S. 2007. [Behavior of the glossopharyngeal and vagus nerves in the retropharyngeal region of sheep: apparent origin in cranium, course, branching and dis-tribution.] Comportamento dos nervos glossofaríngeo e vago, na região retrofaríngea de ovinos: origem aparente no crânio, trajeto, ramificação e distribuição. Pesquisa Veterinária Brasileira 27(3):115-123. Setor de Anatomia dos Animais Domésticos e Silvestres, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia. Universidade de São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-900, Brazil. E-mail: ceambrosio@usp.br In 60 hemiheads of sheep of the Santa Ines breed the apparent origin in the skull of itinerary, ramification and distribution of the glossopharingeal nerve (Gf) and the vagus nerve (Vg) in the retropharyngeal region (Rr) were studied. By fixation and dissection of the specimens it was seen that the glossopharyngeal nerve and the vagus nerve arise from the jugular foramen in 100% of the cases. The right and the left glossopharingeal nerve (Glde) are frequently (86.6%) located more medially to the tympanic bulla, pass caudally to the stylohyoid bone, bypass the margin of the caudal stylopharyngeal muscle, the tonsilla, of the pharyngeal and the lingual mucous membrane. These branches are distributed, respectively, in the carotid sinus, pharyngeal musculature, soft palate, stylopharyngeal muscle, palatine tonsil, pharyngeal mucosa and the caudal third of the tongue (100%). The right and the left vagus nerve run caudally in association with the accessory nerves (Ac) up to the atlas (70%) and go then medially to the occipital artery, dorsally to the common carotid and the sympathetic trunk in the cervical region (80%). The vague nerves have pharyngeal (86.6%) and cranial laryngeal (100%) branches.