Resultado da pesquisa (46)

Termo utilizado na pesquisa development

#41 - Development and evaluation of a recombinant DNA vaccine candidate expressing porcine circovirus 2 structural protein, p.76-82

Abstract in English:

ABSTRACT.- Silva Júnior A., Castro L.A., Chiarelli Neto O., Silva F.M.F., Vidigal P.M.P., Moraes M.P. & Almeida M.R. 2009. Development and evaluation of a recombinant DNA vaccine candidate expressing porcine circovirus 2 structural protein. Pesquisa Veterinária Brasileira 29(1):76-82. Laboratório de Infectologia Molecular Animal, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Av. PH Rolfs s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil. E-mail: marcia@ufv.br Porcine circovirus 2 (PCV2) is generally associated with the porcine circovirosis syndrome, which is considered an important disease of swine and has potentially serious economic impact on the swine industry worldwide. This article describes the construction of a recombinant plasmid expressing the PCV2 structural protein and the evaluation of cellular and humoral immune responses produced by this recombinant vaccine in BALB/c mice. The vaccine candidate was obtained and analyzed in vivo, in an effort to determine the ability to induce a specific immune response in mice. DNA was extracted from a Brazilian PCV2 isolate and the gene coding for Cap protein was amplified by PCR and inserted into an expression plasmid. Groups of BALB/c mice were inoculated intra-muscularly and intradermally in a 15-day interval, with 100 µg and 50 µg of the vaccine construct, respectively. Another group was inoculated intramuscularly with 100 µg of empty plasmid, corresponding to the control group. Seroconversion and cellular response in BALB/c mice were compared and used for vaccine evaluation. Seroconversion was analyzed by ELISA. After a series of 3 immunizations the spleen cells of the immunized animals were used to perform lymphocyte proliferation assays. Seroconversion to PCV2 was detected by ELISA in the animals inoculated with the vaccine construct when compared with control groups. Lymphocyte proliferation assays showed a stronger cell proliferation in the inoculated animals compared with the control group. Thus, the vaccine candidate construct demonstrated to be able to induce both humoral and cellular responses in inoculated mice.

Abstract in Portuguese:

ABSTRACT.- Silva Júnior A., Castro L.A., Chiarelli Neto O., Silva F.M.F., Vidigal P.M.P., Moraes M.P. & Almeida M.R. 2009. Development and evaluation of a recombinant DNA vaccine candidate expressing porcine circovirus 2 structural protein. Pesquisa Veterinária Brasileira 29(1):76-82. Laboratório de Infectologia Molecular Animal, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Av. PH Rolfs s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil. E-mail: marcia@ufv.br Porcine circovirus 2 (PCV2) is generally associated with the porcine circovirosis syndrome, which is considered an important disease of swine and has potentially serious economic impact on the swine industry worldwide. This article describes the construction of a recombinant plasmid expressing the PCV2 structural protein and the evaluation of cellular and humoral immune responses produced by this recombinant vaccine in BALB/c mice. The vaccine candidate was obtained and analyzed in vivo, in an effort to determine the ability to induce a specific immune response in mice. DNA was extracted from a Brazilian PCV2 isolate and the gene coding for Cap protein was amplified by PCR and inserted into an expression plasmid. Groups of BALB/c mice were inoculated intra-muscularly and intradermally in a 15-day interval, with 100 µg and 50 µg of the vaccine construct, respectively. Another group was inoculated intramuscularly with 100 µg of empty plasmid, corresponding to the control group. Seroconversion and cellular response in BALB/c mice were compared and used for vaccine evaluation. Seroconversion was analyzed by ELISA. After a series of 3 immunizations the spleen cells of the immunized animals were used to perform lymphocyte proliferation assays. Seroconversion to PCV2 was detected by ELISA in the animals inoculated with the vaccine construct when compared with control groups. Lymphocyte proliferation assays showed a stronger cell proliferation in the inoculated animals compared with the control group. Thus, the vaccine candidate construct demonstrated to be able to induce both humoral and cellular responses in inoculated mice.


#42 - Development of conditioned taste aversion to Mascagnia rigida in goats, p.571-574

Abstract in English:

ABSTRACT.- Barbosa R.R., Pacífico da Silva I. & Benito Soto-Blanco B. 2008. Development of conditioned taste aversion to Mascagnia rigida in goats. Pesquisa Veterinária Brasileira 28(12):571-574. Programa de Pós-Graduação em Ciência Animal, Universidade Federal Rural do Semi-Árido, BR 110 Km 47, Mossoró, RN 59625-900, Brazil. E-mail: benito.blanco@pq.cnpq.br The aim of this study was to determine whether goats could be averted from consuming Mascagnia rigida, a toxic plant found in the semiarid region of northeastern Brazil. Fourteen male goats not previously familiarized to M. rigida were randomly allocated to two treatment groups: control (treated with 5.5mL water orally by a drenching gun) and lithium group (treated with 100mg LiCl/kg body weight orally by a drenching gun). For conditioning, goats were allowed to feed on M. rigida leaves for 15 min, followed by LiCl or water administration. The time spent on eating M. rigida leaves was measured. The conditioning was repeated daily until the LiCl-treated goats stopped eating M. rigida. On the 10th, 17th, and 24th day after conditioning, extinction trials of the M. rigida aversion were performed in goats by using single-choice tests. There was no difference between the two treatment groups with respect to the consumption of M. rigida on the first day of aversion conditioning, however, controls ingested increasing amounts of the plant on consecutive conditioning days. On the second day, five out of the seven goats in the lithium group did not eat the leaves, but on the third day, all the goats in the lithium group did not ingest M. rigida. This aversion persisted throughout all evaluated days. This indicates that goats can be easily conditioned by using lithium chloride to avoid eating M. rigida temporarily

Abstract in Portuguese:

ABSTRACT.- Barbosa R.R., Pacífico da Silva I. & Benito Soto-Blanco B. 2008. Development of conditioned taste aversion to Mascagnia rigida in goats. Pesquisa Veterinária Brasileira 28(12):571-574. Programa de Pós-Graduação em Ciência Animal, Universidade Federal Rural do Semi-Árido, BR 110 Km 47, Mossoró, RN 59625-900, Brazil. E-mail: benito.blanco@pq.cnpq.br The aim of this study was to determine whether goats could be averted from consuming Mascagnia rigida, a toxic plant found in the semiarid region of northeastern Brazil. Fourteen male goats not previously familiarized to M. rigida were randomly allocated to two treatment groups: control (treated with 5.5mL water orally by a drenching gun) and lithium group (treated with 100mg LiCl/kg body weight orally by a drenching gun). For conditioning, goats were allowed to feed on M. rigida leaves for 15 min, followed by LiCl or water administration. The time spent on eating M. rigida leaves was measured. The conditioning was repeated daily until the LiCl-treated goats stopped eating M. rigida. On the 10th, 17th, and 24th day after conditioning, extinction trials of the M. rigida aversion were performed in goats by using single-choice tests. There was no difference between the two treatment groups with respect to the consumption of M. rigida on the first day of aversion conditioning, however, controls ingested increasing amounts of the plant on consecutive conditioning days. On the second day, five out of the seven goats in the lithium group did not eat the leaves, but on the third day, all the goats in the lithium group did not ingest M. rigida. This aversion persisted throughout all evaluated days. This indicates that goats can be easily conditioned by using lithium chloride to avoid eating M. rigida temporarily


#43 - Technological development of (40mg) furosemide tablets: a equivalence and bioavaibility study in dogs, p.462-466

Abstract in English:

ABSTRACT.- Lima E.Q., Albuquerque M.M., Rodrigues O.G., Alencar J.R.B., Medeiros F.P.M. & Neto P.R. 2007. Technological development of (40mg) furosemide tablets: a equivalence and bioavaibility study in dogs. Pesquisa Veterinária Brasileira 27(11):462.466. Unidade Acadêmica de Medicina Veterinária (UAMV), Centro de Saúde e Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Acadêmica s/n, Santa Cecília, Cx. Postal 64, Patos, PB 58708110, Brazil. E-mail: onaldo@cstr.ufcg.edu.br Furosemide (40mg) was administered to 20 street dogs, 10 males and 10 females, in two different pharmaceutical forms: (1) compressed furosemide 40mg formulated at the Federal University of Pernambuco (UFPE-tablet), and (2) a commercial formulation with equal bioequivalence produced by the Laboratory for Pharmaceutical Technology of Pernambuco State (LAFEPE), the LAFEPE-furosemide. The study aimed to evaluate the kinetics of dissolution of the UFPE-tablet in order to analyze the behavior of bioavailability of the best formulation for veterinary use. The plasmatic concentrations of furosemide for the determination of parameters of pharmacological kinetics were analyzed by high-performance liquid chromato-graphic method (HPLC). The in vitro study accomplished through physiochemical analyses demonstrated that the formulas of the furosemide tablets attained the pharmaceutical requirements in agreement with USP 23 and the Brazilian Pharmacopoeia. The evaluation accomplished in dogs with UFPE-tablets given in only dose demonstrated uniformity in blood levels indicating stability in maintenance of the pharmaceutical formulation and efficiency in absorption of the active compound. These values are not significantly different in relation to the 5% confidence limit. Regarding maximum concentration (Tmax) time and global bioavaibility assessed by AUC means, there were no considerable differences as well. UFPE-furosemide displayed 743.492µg/mL.h as AUC average value whereas LAFEPE-furosemide had an average of 537.284µg/mL.h.

Abstract in Portuguese:

ABSTRACT.- Lima E.Q., Albuquerque M.M., Rodrigues O.G., Alencar J.R.B., Medeiros F.P.M. & Neto P.R. 2007. Technological development of (40mg) furosemide tablets: a equivalence and bioavaibility study in dogs. Pesquisa Veterinária Brasileira 27(11):462.466. Unidade Acadêmica de Medicina Veterinária (UAMV), Centro de Saúde e Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Acadêmica s/n, Santa Cecília, Cx. Postal 64, Patos, PB 58708110, Brazil. E-mail: onaldo@cstr.ufcg.edu.br Furosemide (40mg) was administered to 20 street dogs, 10 males and 10 females, in two different pharmaceutical forms: (1) compressed furosemide 40mg formulated at the Federal University of Pernambuco (UFPE-tablet), and (2) a commercial formulation with equal bioequivalence produced by the Laboratory for Pharmaceutical Technology of Pernambuco State (LAFEPE), the LAFEPE-furosemide. The study aimed to evaluate the kinetics of dissolution of the UFPE-tablet in order to analyze the behavior of bioavailability of the best formulation for veterinary use. The plasmatic concentrations of furosemide for the determination of parameters of pharmacological kinetics were analyzed by high-performance liquid chromato-graphic method (HPLC). The in vitro study accomplished through physiochemical analyses demonstrated that the formulas of the furosemide tablets attained the pharmaceutical requirements in agreement with USP 23 and the Brazilian Pharmacopoeia. The evaluation accomplished in dogs with UFPE-tablets given in only dose demonstrated uniformity in blood levels indicating stability in maintenance of the pharmaceutical formulation and efficiency in absorption of the active compound. These values are not significantly different in relation to the 5% confidence limit. Regarding maximum concentration (Tmax) time and global bioavaibility assessed by AUC means, there were no considerable differences as well. UFPE-furosemide displayed 743.492µg/mL.h as AUC average value whereas LAFEPE-furosemide had an average of 537.284µg/mL.h.


#44 - Development of an enzyme-linked immunosorbent assay for the detection of antibodies against Babesia bovis, 20(4):167-170

Abstract in English:

ABSTRACT.- Madruga C.R., Araújo F.R., Marques A.P.C., Carvalho C.M.E., Cusinato F.Q., Crocci A.J., Kessler R.H. & Miguita M. 2000. [Development of an enzyme-linked immunosorbent assay for the detection of antibodies against Babesia bovis.] Desenvolvimento de uma prova de imunoadsorção enzimática para detecção de anticorpos contra Babesia bovis. Pesquisa Veterinária Brasileira 20(4):167-170. Embrapa Gado de Corte, BR 262 Km 4, Campo Grande, MS 79002-970, Brazil. An enzyme-linked immunosorbent assay (ELISA) for antibodies to Babesia bovis was developed and evaluated in comparison with the indirect fluorescent antibody test (IFAT). The ELISA sensitivity and specificity, estimated with 100 positive sera from cattle experimentally infected with B. bovis and 108 negative sera collected from B. bovis-free herds, were 98.0% and 98.1 %, respectively. Positive and negative predictive values were, respectively, 98.0% and 98.1 %, and precision was 98.1 %. No cross-reactions were detected with 80 sera from calves experimentally inoculated with Babesia bigemina. The ELISA was compared with IFAT using 110 cattle sera from an enzootically stable area and with 168 cattle sera from an enzootically unstable area. In both cases, there was a significant agreement between results of both tests (P=0.631 and 0.4725, respectively). In an epidemiological study performed with ELISA in the Pantanal region of the State of Mato Grosso do Sul with 1,365 cattle sera, 83.9%were positive for antibodies against B. bovis, characterizing this region as enzootically stable.

Abstract in Portuguese:

RESUMO.- Madruga C.R., Araújo F.R., Marques A.P.C., Carvalho C.M.E., Cusinato F.Q., Crocci A.J., Kessler R.H. & Miguita M. 2000. [Development of an enzyme-linked immunosorbent assay for the detection of antibodies against Babesia bovis.] Desenvolvimento de uma prova de imunoadsorção enzimática para detecção de anticorpos contra Babesia bovis. Pesquisa Veterinária Brasileira 20(4):167-170. Embrapa Gado de Corte, BR 262 Km 4, Campo Grande, MS 79002-970, Brazil. Uma prova de imunoadsorção enzimática (ELISA) para detecção de anticorpos contra Babesia bovis foi desenvolvida e avaliada em comparação à imunofluorescência indireta (IFI). A sensibilidade e especificidade do ELISA, determinadas pela análise de 100 soros positivos de bovinos infectados experimentalmente com B. bovis e 108 soros negativos colhidos de bovinos livres de infecção por este hemoparasito, foram de 98,0% e 98, 1 %, respectivamente. Os valores preditivos positivo e negativo foram, respectivamente, 98,0% e 98, 1 % e a precisão do teste foi de 9.8, 1 %. Não foram detectadas reações cruzadas com 80 soros de bezerros experimentalmente inoculados com Babesia bigemina. O ELISA foi comparado à IFI usando 110 soros de rebanhos de área de estabilidade endêmica e 168 soros de rebanhos de áreas de instabilidade endêmica. Em ambos os casos, houve concordância significativa (P=0,631 e 0,4725, respectivamente) entre os resultados demonstrados pelos dois testes. Em um estudo epidemiológico realizado com o ELISA na região do Pantanal de Mato Grosso do Sul, com 1.365 soros de bovinos, 83,9% foram positivos para anticorpos contra B. bovis, caracterizando a região estudada como endemicamente estável.


#45 - Development and standardization of an indirect ELISA. for the serological diagnosis of classical swine fever, 19(3/4):123-127

Abstract in English:

ABSTRACT.- Paredes J.C.M., Oliveira L.G., Braga A.C., Trevisol I.M. & Roehe P.M. 1999. Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever. [Desenvolvimento e padronização de um ELISA indireto para o diagnóstico sorológico de peste suína clássica.] Pesquisa Veterinária Brasileira, 19(3/4):123-127. Equipe de Virologia, FEPAGRO- Centro de Pesquisas Veterinárias Desidério Finamor, Caixa Postal 2076, Porto Alegre, RS 90001-970, Brazil. An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered ·as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was simila1: It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever vírus from those induced by other pestiviruses.

Abstract in Portuguese:

RESUMO.- Paredes J.C.M., Oliveira L.G., Braga A.C., Trevisol I.M. & Roehe P.M. 1999. Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever. [Desenvolvimento e padronização de um ELISA indireto para o diagnóstico sorológico de peste suína clássica.] Pesquisa Veterinária Brasileira, 19(3/4):123-127. Equipe de Virologia, FEPAGRO- Centro de Pesquisas Veterinárias Desidério Finamor, Caixa Postal 2076, Porto Alegre, RS 90001-970, Brazil. Um ensaio imunoenzimático do tipo ELISA indireto (ELISA-I) foi desenvolvido e padronizado para o diagnóstico sorológico de peste suína clássica. Na comparação foram utilizadas novecentas e trinta e sete amostras de soros suínos, as quais foram testadas pelo teste de soroneutralização seguido de revelação por imunoperoxidase (NPLA), tomado como padrão, resultando em 223 amostras positivas e 714 negativas. Em relação ao NPLA, o ELISA-I apresentou sensibilidade de 98,21%, especificidade de 92,86%, valor preditivo positivo de 81, 11%, valor preditivo negativo de 99,4% e precisão de 94, 1 %. A análise estatística dos resultados revelou uma correlação muito forte (r=0,94) entre os dois testes. Quando comparado com um "kit" de ELISA disponível comercialmente, a performance de ambos em relação ao NPLA foi similar. Concluiu-se que o ELISA-I é um teste apropriado para triagem em larga escala de soros para a detecção de anticorpos contra o Vírus da Peste Suína Clássica (VPSC), embora não seja capaz de diferenciar entre anticorpos induzidos pelo VPSC ou outros pestivírus.


#46 - Anomalies in the development of the tubular genital system of slaughtered female buffaloes (Bubalus bubalis)

Abstract in English:

Genital systems of 612 female water buffaloes (Bubalus bubalis Lin.) of undefined breed, obtained from slaughterhouses in the State of Para, were studied. A total of 77 (12.6%) animals showed abnormalities. Results from pregnant and non-pregnant animals were considered separately. In the oviducts of pregnant buffaloes, one case (1.3%) of unilateral segmentary aplasia was identified, while three similar cases (3.9%) were noted in non-pregnant buffaloes, as well as one case (1 .3%) with bilateral involvement. A large percentage (44.2%) of the findings were paraovarian cysts: 26 instances (33.8%) of unilateral paraovarian cysts were identified in the mesosalpinx, 11 (14.3%) corresponding to pregnant and 15 (19.5%) to non-pregnant females; eight bilateral cases (10.4%) were also observed, of which three (3.9%) were found in pregnant and five (6.5%) in non-pregnant buffaloes. In the uterus, out of a total of 29 (37.6%) anomalies, seven (9.1%) appeared in pregnant and 22 (28.5%) in non-pregnant buffaloes. A high prevalence (28.6%) of cystic formation on the perimetrium was observed in these animals. Malformations of the cervix were only found in three (3.9%) non-pregnant buffaloes. Two anomalies (2.6%) of the vagina were encountered in non-pregnant animals. Four (5.2%) anomalies were seen in the vestibule, one (1 .3%) in a pregnant animal and three (3.9%) in non-pregnant animals.

Abstract in Portuguese:

Foram estudados 612 sistemas genitais de búfalas (Bubalus bubalis Lin.) sem raça definida, provenientes de diferentes regiões do Estado do Pará e abatidas em matadouros, detectando-se anomalias em 77 (12,6%) delas. Os resultados foram considerados separadamente para animais gestantes e não gestantes. Nos oviductos de búfalas gestantes foi identificado um caso de aplasia segmentar unilateral (1,3%), enquanto em não gestantes foram assinalados três casos semelhantes (3,9%) e mais um caso bilateral (1,3%). No mesossalpinge, foram detectados 26 casos (33,8%) de cistos paraováricos unilaterais, sendo 11 (14,3%) em animais gestantes e 15 (19,5%) em não gestantes, além de oito (19,4%) casos bilaterais, sendo três (3,9%) em animais gestantes e cinco (6,5%) em não gestantes, perfazendo um total de 44,2% de cistos paraováricos nos achados. No útero foram verificadas 29 (37,6%) anomalias, sendo sete (9,1 %) em animais gestantes e 22 (28,5%) em não gestantes, com alta prevalência (28,6%) de formações císticas no perimétrio. Na cérvice foram encontradas três (3,9%) malformações em animais não gestantes. Na vagina foram encontradas duas (2,6%) anomalias em búfalas não gestantes, enquanto que no vestíbulo foram encontrados quatro (5,2%) casos, sendo um (1,3%) em búfala gestante e três (3,9%) em búfalas não gestantes.


Colégio Brasileiro de Patologia Animal SciELO Brasil CAPES CNPQ UNB UFRRJ CFMV