PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Souza F.N., Blagitz M.G., Latorre A.O., Mori C.S., Sucupira M.C.A. & Della Libera A.M.M.P. 2012. Effects of in vitro selenium supplementation on blood and milk neutrophils from dairy cows. Pesquisa Veterinária Brasileira 32(2):174-178. Departamento de Clínica Médica e Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: nogueirasouza@yahoo.com.br The study was designed to assess the effects of in vitro selenium addition on intracellular hydrogen peroxide production by neutrophils from the milk and blood of dairy cows. Blood from 10 dairy cows and 20 milk samples from five dairy cows were incubated with 0 mg (control) or 10µM of sodium selenite. Then, milk and blood neutrophils were submitted for evaluation of intracellular hydrogen peroxide production by flow cytometry using 2’,7’-dichlorofluorescein diacetate as a probe. The selenium status of the animals was evaluated by determination of the blood glutathione peroxidase activity. The results of the present work showed that in vitro selenium supplementation leads to an enhancement in intracellular hydrogen peroxide production, which indicates an improvement in the bactericidal effects of blood and milk neutrophils even in cows with a selenium-adequate status. Thus, the present study showed that in vitro Se supplementation leads to an enhancement in intracellular hydrogen peroxide production, indicating an improvement in the bactericidal effects of blood and milk neutrophils in cows with Se-adequate status.

• Selenium mastitis selênio mastite

Abstract in Portuguese:

RESUMO.- Souza F.N., Blagitz M.G., Latorre A.O., Mori C.S., Sucupira M.C.A. & Della Libera A.M.M.P. 2012. Effects of in vitro selenium supplementation on blood and milk neutrophils from dairy cows. Pesquisa Veterinária Brasileira 32(2):174-178. Departamento de Clínica Médica e Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: nogueirasouza@yahoo.com.br O presente estudo avaliou o efeito da suplementação in vitro de selênio sobre a produção intracelular de perόxido de hidrogênio (H2O2) por leucόcitos polimorfonucleares do leite e do sangue em bovinos. Assim, 10 e 20 amostras de sangue e leite, respectivamente, foram incubadas com 0 mg (controle) ou 10µM de selenito de sόdio. A determinação da produção intracelular de peróxido de hidrogênio se deu por citometria de fluxo através da utilização do 2´,7´ diclorodihidrofluoresceína diacetato como sonda. A mensuração do conteúdo de selênio foi avaliada pela atividade da glutationa peroxidase eritrocitária. Os leucócitos polimorfonucleares tanto sanguíneos quanto do leite apresentaram significativo aumento na produção intracelular de H2O2 com a suplementação in vitro de selênio. Desta forma, o presente estudo apontou para aumento da produção intracelular de H2O2, indicando aumento da capacidade microbicida dos leucócitos polimorfonucleares sanguíneos e lácteos mesmo em animais com níveis adequados de selênio.

Abstract in English:

ABSTRACT.- Blagitz M.G., Batista C.F., Souza F.N., Benites N.R., Melville P.A., Stricagnolo C.R., Ricciardi M., Gomes V., Azedo M.R., Sanches B.G.S. & Della Libera A.M.M.P. 2008. [Cellular and microbiological profile of Santa Ines ewes in the lactation and the post-weaning period.] Perfil celular e microbiológico de ovelhas Santa Inês no período lactante e pós-desmame. Pesquisa Veterinária Brasileira 28(9):417-422. Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: magblagitz@usp.br The aim was to evaluate and compare the microbiological and cellular profile of the milk of Santa Ines ewes during the lactation period and the active involution. Milk samples were analyzed from 12 ewes during these distinct periods. Clinical examination of the mammary gland, somatic cell count (SCC), California Mastitis Test (CMT), bacteriologic screening and sensibility of the pathogens in vitro were performed. Most alterations were observed in the active involution period. SCC and CMT were higher in this same period. Besides this, a high persistency of infection occurred. The active involution period did not show high susceptibility. Coagulase-negative staphylococci were the only isolated bacteria. A high antimicrobial sensibility of these pathogens was also encountered.

• Mastitis ewes involution lactation staphylococci

Abstract in Portuguese:

ABSTRACT.- Blagitz M.G., Batista C.F., Souza F.N., Benites N.R., Melville P.A., Stricagnolo C.R., Ricciardi M., Gomes V., Azedo M.R., Sanches B.G.S. & Della Libera A.M.M.P. 2008. [Cellular and microbiological profile of Santa Ines ewes in the lactation and the post-weaning period.] Perfil celular e microbiológico de ovelhas Santa Inês no período lactante e pós-desmame. Pesquisa Veterinária Brasileira 28(9):417-422. Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: magblagitz@usp.br The aim was to evaluate and compare the microbiological and cellular profile of the milk of Santa Ines ewes during the lactation period and the active involution. Milk samples were analyzed from 12 ewes during these distinct periods. Clinical examination of the mammary gland, somatic cell count (SCC), California Mastitis Test (CMT), bacteriologic screening and sensibility of the pathogens in vitro were performed. Most alterations were observed in the active involution period. SCC and CMT were higher in this same period. Besides this, a high persistency of infection occurred. The active involution period did not show high susceptibility. Coagulase-negative staphylococci were the only isolated bacteria. A high antimicrobial sensibility of these pathogens was also encountered.