PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Gonçalves L.M.F., Mineiro A.L.B.B., Carvalho S.M., Campos A.P., Evangelista L.S.M., Pinho F.A., Silva S.M.M.S. & Costa F.A.L. 2011. [Search agglutinins, leptospires antigen and apoptosis in the kidney of swine naturally infected with Leptospira spp.] Pesquisa de aglutininas, antígeno de leptospiras e apoptose em rim de suínos naturalmente infectados por Leptospira spp. Pesquisa Veterinária Brasileira 31(7):561-568. Setor de Patologia Animal, Departamento de Clínica e Cirurgia Veterinária, Centro de Ciências Agrárias, Universidade Federal do Piauí, Campus Agrícola da Socopo s/n. Teresina, PI 64.049-550, Brazil. E-mail: fassisle@gmail.com Serum and kidney of 75 mixed bred swine (no definite breed) reared in an extensive system and slaughtered in Timon (state of Maranhão) and Teresina (state of Piauí), Brazil, two cities separated from each other by the Parnaiba River, and 75 crossbred swine from a confinement rearing system, sired by Landrace, Large White and/or Duroc, were used in this study. From the 150 analyzed samples for the microscopic agglutination test (MAT), seven were reagents and the serovar Icterohaemorrhagiae (42.86%) was the most frequent. A comparison between both systems to verify a predisposition to Leptospira spp. infection showed that susceptibility was greater in extensively reared animals than in those bred in confinement (c2 test, p<0.05). Inflammatory infiltrates covered an average area larger in the seropositive revealing a significant difference for the seronegative animals (p<0.05, Mann-Whitney U-Test). Morphometric analysis showed Leptospira spp. and antigen labeling in seropositive animals only (p<0.05, Mann-Whitney U-test). Apoptosis in tubular epithelial cells was significantly more evident in the infected animals compared to uninfected. The rearing system and environment conditions are an important factor in the susceptibility of swine to Leptospira spp. infection. An eventual association of leptospira antigen and apoptotic cells suggests a probable mechanism of renal injury at leptospirosis.

• Leptospira spp. leptospirosis leptospirose

Abstract in Portuguese:

RESUMO.- Gonçalves L.M.F., Mineiro A.L.B.B., Carvalho S.M., Campos A.P., Evangelista L.S.M., Pinho F.A., Silva S.M.M.S. & Costa F.A.L. 2011. [Search agglutinins, leptospires antigen and apoptosis in the kidney of swine naturally infected with Leptospira spp.] Pesquisa de aglutininas, antígeno de leptospiras e apoptose em rim de suínos naturalmente infectados por Leptospira spp. Pesquisa Veterinária Brasileira 31(7):561-568. Setor de Patologia Animal, Departamento de Clínica e Cirurgia Veterinária, Centro de Ciências Agrárias, Universidade Federal do Piauí, Campus Agrícola da Socopo s/n. Teresina, PI 64.049-550, Brazil. E-mail: fassisle@gmail.com Soro e rim de 75 suínos mestiços sem raça definida, criados em sistema extensivo e abatidos em Timon (MA) e Teresina (Piauí), Brasil, duas cidades separadas pelo Rio Parnaíba e 75 suínos mestiços de um sistema de criação em confinamento, filhos de Landrace, Large White e/ou Duroc foram utilizados neste estudo. Das 150 amostras analisadas pela prova de Soroaglutinação Microscópica (SAM), sete foram reagentes e o sorovar Icterohaemorrhagiae (42,86%) foi o mais frequente. Uma comparação entre os dois sistemas para examinar uma predisposição para infecção para Leptospira spp. mostrou que a suscetibilidade foi maior nos animais criados extensivamente do que naqueles criados em confinamento (teste c2, p<0,05). A presença de infiltrado inflamatório foi significantemente maior nos animais soropositivos comparados aos soronegativos (p<0,05, Teste U de Mann-Whitney). A análise morfométrica mostrou Leptospira spp. e o antígeno de leptospira apenas nos animais soropositivos (p<0,05, teste de U de Mann-Whitney). Apoptose em células epiteliais tubulares foi significantemente mais evidente nos animais infectados comparados aos não infectados (p<0,05, Teste U de Mann-Whitney). Uma eventual associação de antígeno de Leptospira e células epiteliais em apoptose sugere um provável mecanismo de lesão renal na leptospirose suína.

Abstract in English:

ABSTRACT.- Arduino G.G.C., Girio R.J.S., Magajevski F.S. & Pereira G.T. 2009. [Agglutinating antibody titers induced by commercial vaccines against bovine leptospirosis.] Títulos de anticorpos aglutinantes induzidos por vacinas comerciais contra leptospirose bovina. Pesquisa Veterinária Brasileira 29(7):575-582. Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, Brazil. E-mail: cocaarduino@yahoo.com.br In the investigation 100 heifers were used, divided into 5 groups of 20 animals each. The four experimental groups were vaccinated using distinct commercial polyvalent bacterines: A, B, C and D, and a group was the control. Samples were collected at days 0, 3, 7, 14, 21, 28, 35, 42, 42, 56, 63, 70, 77, 84, 91, 120, 150 and 180 from the first injection of the vaccine. The selection of the animals for the experimental groups was done based on a serological screening with 6 antigens of Leptospira sp. constituted by non-reagent animals. The vaccine titers were monitored using the microscopic agglutination test (MAT) for Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, Pomona and Wolffi serovars. All vaccines used were capable to product agglutinins for the Hardjo and Wolffi serovars observed at 3 days after vaccination, remaining until the 150th day; those serovars induced the highest titres of agglutinins. Vaccine D, in spite of not containing the Wolffi serovar, induced the production of agglutinins to this serovar. Agglutinins to the Canicola serovar were only observed in the animals vaccinated with the D bacterine. Vaccine D induced the highest average titers of antibodies to all tested serovars.

• Leptospirosis cattle vaccine agglutinins

Abstract in Portuguese:

ABSTRACT.- Arduino G.G.C., Girio R.J.S., Magajevski F.S. & Pereira G.T. 2009. [Agglutinating antibody titers induced by commercial vaccines against bovine leptospirosis.] Títulos de anticorpos aglutinantes induzidos por vacinas comerciais contra leptospirose bovina. Pesquisa Veterinária Brasileira 29(7):575-582. Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, Brazil. E-mail: cocaarduino@yahoo.com.br In the investigation 100 heifers were used, divided into 5 groups of 20 animals each. The four experimental groups were vaccinated using distinct commercial polyvalent bacterines: A, B, C and D, and a group was the control. Samples were collected at days 0, 3, 7, 14, 21, 28, 35, 42, 42, 56, 63, 70, 77, 84, 91, 120, 150 and 180 from the first injection of the vaccine. The selection of the animals for the experimental groups was done based on a serological screening with 6 antigens of Leptospira sp. constituted by non-reagent animals. The vaccine titers were monitored using the microscopic agglutination test (MAT) for Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, Pomona and Wolffi serovars. All vaccines used were capable to product agglutinins for the Hardjo and Wolffi serovars observed at 3 days after vaccination, remaining until the 150th day; those serovars induced the highest titres of agglutinins. Vaccine D, in spite of not containing the Wolffi serovar, induced the production of agglutinins to this serovar. Agglutinins to the Canicola serovar were only observed in the animals vaccinated with the D bacterine. Vaccine D induced the highest average titers of antibodies to all tested serovars.

Abstract in English:

ABSTRACT.- Silva E.F., Seyffert N., Jouglard S.D.D., Athanazio D.A., Dellagostin O.A. & Brod C.S. 2009. [Seroprevalence of leptospiral infection in capybaras (Hydrochoerus hydrochaeris) in a slaughterhouse of Rio Grande do Sul, Brazil.] Soroprevalência da infecção leptospiral em capivaras (Hydrochoerus hydrochaeris) abatidas em um frigorífico do Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(8):174-176. Laboratório do Centro de Controle de Zoonoses, Faculdade de Veterinária, Universidade Federal de Pelotas, 96010-900 Pelotas, RS, Brazil. E-mail: efsilva@ufpel.edu.br Capybaras (Hydrochoerus hydrochaeris) are wild rodents from the American Continent with increasing importance as a commercial alternative source of meat for human consumption. Studies on seroprevalence for leptospiral infection are scarce and restricted to free living capybaras. We report detection of agglutinating antibodies against leptospires in 27% (6/22) of all animals in a slaughterhouse from Rio Grande do Sul. The highest antibody titers predicted Australis as the infecting serogroup due to reactions against a reference strain of serovar Bratislava and a canine local isolate of serovar Australis, characterized as Leptospira noguchii. The data presented in this report highlight that a considerable fraction of capybaras in captivity may behave as reservoir for pathogenic leptospires emphasizing the occupational risk of those who deal with animal farming and slaughter.

• Leptospirosis capybara serology agglutinins

Abstract in Portuguese:

ABSTRACT.- Silva E.F., Seyffert N., Jouglard S.D.D., Athanazio D.A., Dellagostin O.A. & Brod C.S. 2009. [Seroprevalence of leptospiral infection in capybaras (Hydrochoerus hydrochaeris) in a slaughterhouse of Rio Grande do Sul, Brazil.] Soroprevalência da infecção leptospiral em capivaras (Hydrochoerus hydrochaeris) abatidas em um frigorífico do Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(8):174-176. Laboratório do Centro de Controle de Zoonoses, Faculdade de Veterinária, Universidade Federal de Pelotas, 96010-900 Pelotas, RS, Brazil. E-mail: efsilva@ufpel.edu.br Capybaras (Hydrochoerus hydrochaeris) are wild rodents from the American Continent with increasing importance as a commercial alternative source of meat for human consumption. Studies on seroprevalence for leptospiral infection are scarce and restricted to free living capybaras. We report detection of agglutinating antibodies against leptospires in 27% (6/22) of all animals in a slaughterhouse from Rio Grande do Sul. The highest antibody titers predicted Australis as the infecting serogroup due to reactions against a reference strain of serovar Bratislava and a canine local isolate of serovar Australis, characterized as Leptospira noguchii. The data presented in this report highlight that a considerable fraction of capybaras in captivity may behave as reservoir for pathogenic leptospires emphasizing the occupational risk of those who deal with animal farming and slaughter.

Abstract in English:

ABSTRACT.- Lázaro N.S. & Hofer E. 1997.Anti-Yersinia enterocolitica serotype 3 agglutinins in swine sera from Rio de Janeiro.[Aglutininas anti-Yersinia enterocolitica 0:3 em soros de suínos do Rio de Janeiro.] Pesquisa Veterinária Brasileira 17(1):9-11. Depto Epidemiologia e Saúde Pública, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23851-970, Brazil. The slow serum agglutination test was applied to 119 healthy pigs for the determination of the possible presence of anti-Yersinia enterocolitica 0:3 agglutinins. Of the 63.9% reactive animals (&ge; 1 :20), 8.4% presented positive titers (&ge;1 :80), suggesting the presence of this pathogen among swine and consequently an additional public health problem.

• Yersinia enterocolitica agglutinins swine aglutininas suíno.

Abstract in Portuguese:

SINOPSE.- Lázaro N.S. & Hofer E. 1997.Anti-Yersinia enterocolitica serotype 3 agglutinins in swine sera from Rio de Janeiro.[ Aglutininas anti-Yersinia enterocolitica 0:3 em soros de suínos do Rio de Janeiro.] Pesquisa Veterinária Brasileira 17(1):9-11. Depto Epidemiologia e Saúde Pública, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23851-970, Brazil. Aglutininas anti-Yersinia enterocolitica 0:3 em soros de suínos do Rio de Janeiro. A pesquisa de aglutininas anti-Yersinia enterocolitica 0:3 foi realizada em 119 suínos sadios, através da prova de soro-aglutinação lenta. Dos 63,9% animais reagentes (&ge;1:20), 8,4% apresentaram títulos a nível de positivo (&ge;1:80) sugerindo a presença deste patógeno em nossos rebanhos suínos constituindo-se consequentemente em mais um problema de saúde pública.