Resultado da pesquisa (4)
Termo utilizado na pesquisa allergy
#1 - Evaluation of skin prick test, exclusion diet and dietary challenge in the diagnosis of food allergy in dogs with chronic pruritus
Abstract in English:
Allergic dogs are commonly sensitized to food allergens. We evaluated the use of the prick test to diagnose food allergies in dogs with pruritus and the efficacy of an exclusion diet based on the test. The prick test was performed in 10 healthy dogs and 34 dogs with pruritus, of which 25 received an exclusion diet for 60 days and was challenged with positive food on the test. pVAS and CADESI-4 were assessed on days 0, 30, and 60 after re-exposure. As a result, two control group dogs reacted to a single food allergen, milk and wheat. Of the 25 dogs with pruritus that reacted to food allergens, 24 (96%) reacted to more than one food allergen, and only one (4%) reacted to a single food protein: pork. In the test group (n=25), there was a significant improvement of pVAS and CADESI-4 after 30 and 60 days of dietary exclusion, with significant worsening of the scores with food allergen challenge. In conclusion, the prick test can be used for screening food allergens to make an exclusion diet.
Abstract in Portuguese:
Cães alérgicos são comumente sensibilizados a alérgenos alimentares. Nós avaliamos o uso do teste de puntura para o diagnóstico de alergias alimentares em cães com prurido e a eficácia de uma dieta de exclusão baseada no teste. O teste de puntura foi realizado em 10 cães saudáveis e em 34 cães com prurido, dos quais 25 receberam uma dieta de exclusão por 60 dias e depois desafiados com o alimento que foi positivo no teste. pVAS e CADESI-4 foram realizados nos dias 0, 30, 60 e depois da reexposição. Como resultados, dois cães do grupo controle reagiram a um único alérgeno alimentar, leite e trigo. Dos 25 cães com prurido que reagiram aos alérgenos alimentares, 24 (96%) reagiram a mais de um alérgeno alimentar e apenas um (4%) reagiu a uma única proteína, que foi porco. No grupo teste (n=25), houve uma significativa melhora do pVAS e CADESI-4 depois de 30 e 60 dias de dieta de exclusão, com significante piora dos scores com o desafio alimentar. Como conclusão, o teste de puntura pode ser usado para selecionar alérgenos alimentares para fazer uma dieta de exclusão.
#2 - Efficacy of hydrolyzed soy dog food and homemade food with original protein in the control of food-induced atopic dermatitis in dogs
Abstract in English:
Trophoallergens are specific components of food or its ingredients, able to precipitate the atopic eczema at 19.6% to 30% of the dogs with atopic dermatitis (AD). This study evaluated the efficacy of hydrolyzed soy dog food and homemade food with unusual protein in the control of chronic pruritus in dogs with AD. For this, twenty-eight dogs with AD were selected. AD diagnosis was based on Favrot’s criteria. The animals were separated in two groups; one group consumed hydrolyzed soy dog food while the other group consumed homemade food with protein sources and original carbohydrates. They were evaluated every two weeks by the Rybnicek and CADLI scale over 60 days. Animals in the group that consumed hydrolyzed soy dog food presented a reduced score of pruritus (Rybnicek scale) on days +15, +30, +45 and +60 (P<0.01) compared to day 0. While the dogs in the homemade food group have not presented a significant difference (P>0.05) in 60 days of treatment. When evaluated by the Canine Atopic Dermatitis Lesion Index (CADLI), dogs treated with soy hydrolyzed dog food had a partial improvement on days +45 (P<0.05) and +60 (P<0.01) compared to day 0, while the dogs in the second group did not show improvements (P>0.05) in 60 days of treatment. In conclusion, soy hydrolyzed dog food has proved effective to partially control clinical signs of food-induced atopic dermatitis; however, it is not effective for the complete control of the disease.
Abstract in Portuguese:
Os trofoalérgenos são componentes específicos do alimento ou de seus ingredientes, capazes de precipitar o eczema atópico em 19,6% a 30% dos cães com dermatite atópica (DA). O presente estudo teve como objetivo avaliar a eficácia da ração de soja hidrolisada e da comida caseira com proteína não usual no controle do prurido crônico em cães com DA. Para isso foram utilizados vinte e oito cães com DA. O diagnóstico de DA foi baseado nos critérios de Favrot. Os animais foram separados em dois grupos, um grupo consumindo ração hidrolisada de soja e o outro grupo comida caseira com fontes de proteína e carboidratos originais. Estes foram avaliados quinzenalmente pela escala de Rybnicek e CADLI durante 60 dias. Os animais do grupo alimentado com ração hidrolisada de soja apresentaram uma minimização no escore de prurido (escala de Rybnicek) nos dias +15, +30, +45 e +60 (P<0,01) em relação ao dia 0. Já os cães do grupo alimentado com comida caseira não apresentaram diferença significativa (P>0,05) nos 60 dias de tratamento. Quando avaliados pelo índice de CADLl os cães tratados com ração hidrolisada de soja tiveram uma melhora parcial nos dias, +45 (P<0,05) e +60 (P<0,01) em relação ao dia 0, enquanto que os cães do segundo grupo não obtiveram melhora (P>0,05) nos 60 dias de tratamento. A ração hidrolisada de soja se mostrou efetiva para controlar parcialmente os sinais clínicos da dermatite atópica induzida por alimentos, no entanto, não é eficaz para o controle total da doença.
#3 - Complement activation-related pseudoallergy in dogs following intravenous administration of a liposomal formulation of meglumine antimoniate, 33(8):1016-1020
Abstract in English:
ABSTRACT.- Ribeiro R.R., Moura E.P., Sampaio W.M., Silva S.M., Fulgêncio G.O., Tafuri W.L., Michalick M.S.M. & Frézard F. 2013. Complement activation-related pseudoallergy in dogs following intravenous administration of a liposomal formulation of meglumine antimoniate. Pesquisa Veterinária Brasileira 33(8):1016-1020. Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Campus Universitário Cruz das Almas s/n, Cruz das Almas, BA 44380-000, Brazil. E-mail: raul@ufrb.edu.br
The increasing use of nanotechnologies in advanced therapies has allowed the observation of specific adverse reactions related to nanostructures. The toxicity of a novel liposome formulation of meglumine antimoniate in dogs with visceral leishmaniasis after single dose has been investigated. Groups of 12 animals received by the intravenous route a single dose of liposomal meglumine antimoniate (group I [GI], 6.5 mg Sb/kg), empty liposomes (GII) or isotonic saline (GIII). Evaluation of hematological and biochemical parameters showed no significant changes 4 days after administration. No undesired effects were registered in the GIII. However, adverse reactions were observed in 67.7% of dogs from both groups that received liposomal formulations. The side effects began moments after bolus administration and disappeared during the first 15 minutes after treatment. Prostation, sialorrhea and defecation were the most frequent clinical signs, registered in 33.3% and 41.6 % of animals from the groups GI and GII, respectively. Tachypnea, mydriasis, miosis, vomiting and cyanosis were also registered in both groups. The adverse reactions observed in this study were attributed to the activation of the complement system by lipid vesicles in a phenomenon known as Complement Activation-Related Pseudoallergy (CARPA). The influence of the physical-chemical characteristics of liposomal formulation in the triggering of CARPA is discussed.
Abstract in Portuguese:
RESUMO.- Ribeiro R.R., Moura E.P., Sampaio W.M., Silva S.M., Fulgêncio G.O., Tafuri W.L., Michalick M.S.M. & Frézard F. 2013. Complement activation-related pseudoallergy in dogs following intravenous administration of a liposomal formulation of meglumine antimoniate. [Pseudoalergia relacionada à ativação do complemento em cães após administração intravenosa de uma formulação lipossomal de antimoniato de meglumina.] Pesquisa Veterinária Brasileira 33(8):1016-1020. Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Campus Universitário Cruz das Almas s/n, Cruz das Almas, BA 44380-000, Brazil. E-mail: raul@ufrb.edu.br
O crescente uso das nanotecnologias nas terapias avançadas tem permitido a observação de reações adversas específicas relacionadas às nanoestruturas. A toxicidade de uma nova formulação lipossomal de antimoniato de meglumina após dose única foi avaliada em cães com leishmaniose visceral. Grupos de 12 animais receberam por via intravenosa uma dose única de antimoniato de meglumina lipossomal (grupo I [GI], 6,5 mg Sb/kg), lipossomas vazios (GII) ou solução salina isotônica (GIII). A avaliação de parâmetros hematológicos e bioquímicos não revelou alterações significativas quatro dias após a administração. Nenhum efeito indesejável foi registrado no GIII. No entanto, reações adversas foram observadas em 67,7% dos cães de ambos os grupos que receberam formulações lipossomais. Os efeitos colaterais iniciaram momentos após a administração em “bolus” e desapareceram no decurso dos primeiros 15 minutos após o tratamento. Prostração, sialorréia e defecação foram os sinais clínicos mais frequentes, registrados em 33,3% e 41,6% dos animais dos grupos GI e GII, respectivamente. Taquipnéia, midríase, miose, vômitos e cianose também foram registrados em ambos os grupos. As reações adversas observadas neste trabalho foram atribuídas à ativação do sistema complemento pelas vesículas lipídicas em fenômeno conhecido como Pseudoalergia Relacionada à Ativação do Complemento (PARAC). A influência das características físico-químicas da formulação lipossomal no desencadeamento de PARAC é abordada.
#4 - Evaluation of skin sensitivity in dogs bearing allergic dermatitis to standardized allergenic extracts of house dust mites, p.341-344
Abstract in English:
ABSTRACT.- Cunha V.E. S., Hahnstadt R.L., Soares A.M.B. & Faccini J.L.H. 2007. Evaluation of skin sensitivity in dogs bearing allergic dermatitis to standardized allergenic extracts of house dust mites. Pesquisa Veterinária Brasileira 27(8):341-344. Curso de Pós-Graduação em Ciência Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil E-mail: mvvictor@ufrrj.br
The objective of the study was to evaluate whether allergenic extracts of five house dust and storage mite species standardized for humans might be used for the diagnosis of canine atopic dermatitis (CAD). Extracts of Dermatophagoides pteronyssinus (Pyroglyphidae), D. farinae (Pyroglyphidae), Blomia tropicalis (Glycyphagidae), Lepidoglyphus destructor (Glycyphagidae) and Tyrophagus putrescentiae (Acaridae) were evaluated by intradermal testing in 20 healthy dogs (control) and 25 dogs with allergic dermatitis. A significant difference in the response was observed between the two groups (p<0.05). Only one dog (5%) in the control group reacted to the intradermal test, whereas 14 dogs (56%) in the allergic group were positive for at least one extract (odds ratio = 24.2). Most of the positive reactions observed in the allergic group occurred against the extracts of T. putrescentiae or L. destructor, each inducing reactions in 10 dogs (40%). D. farinae, D. pteronyssinus e B. tropicalis extracts induced reactions in 7 (28%), 3 (12%) and 3 (12%) dogs, respectively. The allergenic extracts standardized for humans evaluated in the present study may be used as a tool to complement the diagnosis of the disease, as well as to select potential allergen candidates for allergen-specific immunotherapy.
Abstract in Portuguese:
ABSTRACT.- Cunha V.E. S., Hahnstadt R.L., Soares A.M.B. & Faccini J.L.H. 2007. Evaluation of skin sensitivity in dogs bearing allergic dermatitis to standardized allergenic extracts of house dust mites. Pesquisa Veterinária Brasileira 27(8):341-344. Curso de Pós-Graduação em Ciência Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil E-mail: mvvictor@ufrrj.br
The objective of the study was to evaluate whether allergenic extracts of five house dust and storage mite species standardized for humans might be used for the diagnosis of canine atopic dermatitis (CAD). Extracts of Dermatophagoides pteronyssinus (Pyroglyphidae), D. farinae (Pyroglyphidae), Blomia tropicalis (Glycyphagidae), Lepidoglyphus destructor (Glycyphagidae) and Tyrophagus putrescentiae (Acaridae) were evaluated by intradermal testing in 20 healthy dogs (control) and 25 dogs with allergic dermatitis. A significant difference in the response was observed between the two groups (p<0.05). Only one dog (5%) in the control group reacted to the intradermal test, whereas 14 dogs (56%) in the allergic group were positive for at least one extract (odds ratio = 24.2). Most of the positive reactions observed in the allergic group occurred against the extracts of T. putrescentiae or L. destructor, each inducing reactions in 10 dogs (40%). D. farinae, D. pteronyssinus e B. tropicalis extracts induced reactions in 7 (28%), 3 (12%) and 3 (12%) dogs, respectively. The allergenic extracts standardized for humans evaluated in the present study may be used as a tool to complement the diagnosis of the disease, as well as to select potential allergen candidates for allergen-specific immunotherapy.
